ID   NTRY_AZOC5              Reviewed;         771 AA.
AC   Q04850; A8IBK4;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   25-MAY-2022, entry version 142.
DE   RecName: Full=Nitrogen regulation protein NtrY;
DE            EC=2.7.13.3;
GN   Name=ntrY; OrderedLocusNames=AZC_3084;
OS   Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / LMG
OS   6465 / NBRC 14845 / NCIMB 13405 / ORS 571).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Xanthobacteraceae; Azorhizobium.
OX   NCBI_TaxID=438753;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1661370; DOI=10.1007/bf00293830;
RA   Pawlowski K., Klosse U., de Bruijn F.J.;
RT   "Characterization of a novel Azorhizobium caulinodans ORS571 two-component
RT   regulatory system, NtrY/NtrX, involved in nitrogen fixation and
RT   metabolism.";
RL   Mol. Gen. Genet. 231:124-138(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43989 / DSM 5975 / JCM 20966 / LMG 6465 / NBRC 14845 / NCIMB
RC   13405 / ORS 571;
RA   Lee K.B., Backer P.D., Aono T., Liu C.T., Suzuki S., Suzuki T., Kaneko T.,
RA   Yamada M., Tabata S., Kupfer D.M., Najar F.Z., Wiley G.B., Roe B.,
RA   Binnewies T., Ussery D., Vereecke D., Gevers D., Holsters M., Oyaizu H.;
RT   "Complete genome sequence of the nitrogen-fixing bacterium Azorhizobium
RT   caulinodans ORS571.";
RL   Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Member of the two-component regulatory system NtrY/NtrX
CC       involved in nitrogen level control. Probably activates NtrX by
CC       phosphorylation.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC         histidine.; EC=2.7.13.3;
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
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DR   EMBL; X63841; CAA45330.1; -; Genomic_DNA.
DR   EMBL; AP009384; BAF89082.1; -; Genomic_DNA.
DR   PIR; S18624; S18624.
DR   RefSeq; WP_012171608.1; NC_009937.1.
DR   AlphaFoldDB; Q04850; -.
DR   SMR; Q04850; -.
DR   STRING; 438753.AZC_3084; -.
DR   PRIDE; Q04850; -.
DR   EnsemblBacteria; BAF89082; BAF89082; AZC_3084.
DR   KEGG; azc:AZC_3084; -.
DR   eggNOG; COG5000; Bacteria.
DR   HOGENOM; CLU_019564_1_0_5; -.
DR   OMA; SKFARMP; -.
DR   OrthoDB; 692375at2; -.
DR   BRENDA; 2.7.13.3; 609.
DR   PRO; PR:Q04850; -.
DR   Proteomes; UP000000270; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR   GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00082; HisKA; 1.
DR   CDD; cd00130; PAS; 1.
DR   Gene3D; 3.30.565.10; -; 1.
DR   InterPro; IPR003660; HAMP_dom.
DR   InterPro; IPR003594; HATPase_C.
DR   InterPro; IPR036890; HATPase_C_sf.
DR   InterPro; IPR005467; His_kinase_dom.
DR   InterPro; IPR003661; HisK_dim/P.
DR   InterPro; IPR036097; HisK_dim/P_sf.
DR   InterPro; IPR017232; NtrY.
DR   InterPro; IPR045671; NtrY-like_N.
DR   InterPro; IPR000014; PAS.
DR   InterPro; IPR035965; PAS-like_dom_sf.
DR   InterPro; IPR013767; PAS_fold.
DR   InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR   Pfam; PF00672; HAMP; 1.
DR   Pfam; PF02518; HATPase_c; 1.
DR   Pfam; PF00512; HisKA; 1.
DR   Pfam; PF19312; NtrY_N; 1.
DR   Pfam; PF00989; PAS; 1.
DR   PIRSF; PIRSF037532; STHK_NtrY; 1.
DR   PRINTS; PR00344; BCTRLSENSOR.
DR   SMART; SM00304; HAMP; 1.
DR   SMART; SM00387; HATPase_c; 1.
DR   SMART; SM00388; HisKA; 1.
DR   SMART; SM00091; PAS; 1.
DR   SUPFAM; SSF47384; SSF47384; 1.
DR   SUPFAM; SSF55785; SSF55785; 1.
DR   SUPFAM; SSF55874; SSF55874; 1.
DR   TIGRFAMs; TIGR00229; sensory_box; 1.
DR   PROSITE; PS50885; HAMP; 1.
DR   PROSITE; PS50109; HIS_KIN; 1.
DR   PROSITE; PS50112; PAS; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell membrane; Kinase; Membrane; Nitrogen fixation;
KW   Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase;
KW   Transmembrane; Transmembrane helix; Two-component regulatory system.
FT   CHAIN           1..771
FT                   /note="Nitrogen regulation protein NtrY"
FT                   /id="PRO_0000074833"
FT   TRANSMEM        22..46
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        56..76
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        99..122
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        295..319
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          321..374
FT                   /note="HAMP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00102"
FT   DOMAIN          386..458
FT                   /note="PAS"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00140"
FT   DOMAIN          511..728
FT                   /note="Histidine kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT   REGION          727..771
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         514
FT                   /note="Phosphohistidine; by autocatalysis"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
SQ   SEQUENCE   771 AA;  84264 MW;  97CFC14504A43309 CRC64;
     MTQAAFDQAS DNGPMTPSGS SFGLFAPAVV LLALISALAT FLILMGLTPV VPTHQVVISV
     LLVNAAAVLI LSAMVGREIW RIAKARARGR AAARLHIRIV GLFAVVSVVP AILVAVVASL
     TLDRGLDRWF SMRTQEIVAS SVSVAQTYVR EHALNIRGDI LAMSADLTRL KSVYEGDRSR
     FNQILTAQAA LRNLPGAMLI RRDLSVVERA NVNIGREFIV PANLAIGDAT PDQPVIYLPN
     DADYVAAVVP LKDYDDLYLY VARLIDPRVI GYLKTTQETL ADYRSLEERR FGVQVAFALM
     YAVITLIVLL SAVWLGLNFS KWLVAPIRRL MSAADHVAEG NLDVRVPIYR AEGDLASLAE
     TFNKMTHELR SQREAILTAR DQIDSRRRFT EAVLSGVGAG VIGLDSQERI TILNRSAERL
     LGLSEVEALH RHLAEVVPET AGLLEEAEHA RQRSVQGNIT LTRDGRERVF AVRVTTEQSP
     EAEHGWVVTL DDITELISAQ RTSAWADVAR RIAHEIKNPL TPIQLSAERL KRKFGRHVTQ
     DREIFDQCTD TIIRQVGDIG RMVDEFSSFA RMPKPVVDSQ DMSEIIRQTV FLMRVGHPEV
     VFDSEVPPAM PARFDRRLVS QALTNILKNA AEAIEAVPPD VRGQGRIRVS ANRVGEDLVI
     DIIDNGTGLP QESRNRLLEP YVTTREKGTG LGLAIVGKIM EEHGGGIELN DAPEGRGAWI
     RLTLKAEGPK AEPTDASTKA TGAATPAAPA ASAMARDAAA DSAARGKNER T