NTT2_ENCCU
ID NTT2_ENCCU Reviewed; 553 AA.
AC Q8SUF9;
DT 01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 25-MAY-2022, entry version 75.
DE RecName: Full=ADP,ATP carrier protein 2;
DE AltName: Full=ADP/ATP translocase 2;
DE AltName: Full=Nucleotide transporter 2;
GN Name=NTT2; OrderedLocusNames=ECU10_0540;
OS Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite).
OC Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Unikaryonidae;
OC Encephalitozoon.
OX NCBI_TaxID=284813;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, DEVELOPMENTAL
RP STAGE, FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=18449191; DOI=10.1038/nature06903;
RA Tsaousis A.D., Kunji E.R.S., Goldberg A.V., Lucocq J.M., Hirt R.P.,
RA Embley T.M.;
RT "A novel route for ATP acquisition by the remnant mitochondria of
RT Encephalitozoon cuniculi.";
RL Nature 453:553-556(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GB-M1;
RX PubMed=11719806; DOI=10.1038/35106579;
RA Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F.,
RA Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P.,
RA Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J.,
RA Vivares C.P.;
RT "Genome sequence and gene compaction of the eukaryote parasite
RT Encephalitozoon cuniculi.";
RL Nature 414:450-453(2001).
CC -!- FUNCTION: ATP transporter involved in the uptake of ATP from the host
CC cell cytoplasm. Provides the microsporidian cell with host ATP in
CC exchange for ADP. This is an obligate exchange system. This energy
CC acquiring activity is an important component of microsporidian
CC parasitism. {ECO:0000269|PubMed:18449191}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=19.8 uM for ATP uptake {ECO:0000269|PubMed:18449191};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:18449191};
CC Multi-pass membrane protein {ECO:0000269|PubMed:18449191}. Note=Only
CC found on the surface of parasites living inside host cells.
CC -!- DEVELOPMENTAL STAGE: Expressed in all stages of development including
CC spores. {ECO:0000269|PubMed:18449191}.
CC -!- SIMILARITY: Belongs to the ADP/ATP translocase tlc family.
CC {ECO:0000305}.
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DR EMBL; EU040267; ABW20408.1; -; Genomic_DNA.
DR EMBL; AL590449; CAD25773.1; -; Genomic_DNA.
DR RefSeq; NP_586169.1; NM_001042002.1.
DR AlphaFoldDB; Q8SUF9; -.
DR TCDB; 2.A.12.1.11; the atp:adp antiporter (aaa) family.
DR PRIDE; Q8SUF9; -.
DR GeneID; 859818; -.
DR KEGG; ecu:ECU10_0540; -.
DR VEuPathDB; MicrosporidiaDB:ECU10_0540; -.
DR HOGENOM; CLU_023964_1_0_1; -.
DR InParanoid; Q8SUF9; -.
DR OMA; FLMTAIY; -.
DR OrthoDB; 1270398at2759; -.
DR Proteomes; UP000000819; Chromosome X.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0005471; F:ATP:ADP antiporter activity; IEA:InterPro.
DR InterPro; IPR004667; ADP_ATP_car_bac_type.
DR PANTHER; PTHR31187; PTHR31187; 1.
DR Pfam; PF03219; TLC; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cell membrane; Glycoprotein; Membrane; Nucleotide-binding;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..553
FT /note="ADP,ATP carrier protein 2"
FT /id="PRO_0000382925"
FT TRANSMEM 44..64
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 115..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 154..174
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..196
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 210..230
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 244..264
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..328
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 362..382
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 385..405
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 477..499
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 507..527
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 27
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 406
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 532
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 553 AA; 62160 MW; 5091200D1F656BF5 CRC64;
MSEIGSSVPV NENRPLLTED EVEAQANSST VWPLSRIRVA RCEWKLWGSL AFIFGASAYI
YSFSRVMKDS FVLSRQLPIA ISFLKTCFVL PISVIVTGIV QKLLVTRTIS KVFDYTLIAF
SFLYLLIGMV LLPFAEKIQP GLYFSRDIFA DDKMAYKGFE FLFAIFLIFN EWTTSFVYVC
AELFGSLVVQ FMFLAFANEA LTIRQSTRMM PLFYVISNIL LLLSSESTSF YSKKVREWDY
KKTCLITNSF FAVFGAMIAV TYLVKKYAEN TILKKQLFIR TEGVAKKKGR KSSAGFSESM
KLMAQSKFLV AMVMNALFYY AGTNLIESSW KNGISVAADA NNMEKRAYSA SIVSGEQRVV
GALVAIILLT PISTLVQTHG WITMAIVPPL VTLVSSLVIF GSAFFNYSNY PEGKTSVILS
SLVKGYKPNF YLECNIGIYC VSGMKIAKYA FYDISKEAIS LQIDPLYRPR LKAVYDGLCG
KLGKSIGSLY AMFWSVMGYN DVRAAAPITL GMWLIISPIW IYSVIYLNRK YNQSIQTSSP
IDLDLFSGKK DLE
