ID   NTT3_ENCCU              Reviewed;         543 AA.
AC   Q8SUG0;
DT   01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=ADP,ATP carrier protein 3;
DE   AltName: Full=ADP/ATP translocase 3;
DE   AltName: Full=Nucleotide transporter 3;
GN   Name=NTT3; OrderedLocusNames=ECU10_0520;
OS   Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite).
OC   Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Unikaryonidae;
OC   Encephalitozoon.
OX   NCBI_TaxID=284813;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, FUNCTION, AND
RP   BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=18449191; DOI=10.1038/nature06903;
RA   Tsaousis A.D., Kunji E.R.S., Goldberg A.V., Lucocq J.M., Hirt R.P.,
RA   Embley T.M.;
RT   "A novel route for ATP acquisition by the remnant mitochondria of
RT   Encephalitozoon cuniculi.";
RL   Nature 453:553-556(2008).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GB-M1;
RX   PubMed=11719806; DOI=10.1038/35106579;
RA   Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F.,
RA   Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P.,
RA   Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J.,
RA   Vivares C.P.;
RT   "Genome sequence and gene compaction of the eukaryote parasite
RT   Encephalitozoon cuniculi.";
RL   Nature 414:450-453(2001).
CC   -!- FUNCTION: ATP transporter involved in the uptake of ATP from the
CC       parasite cell cytoplasm into the mitosome matrix. Equilibrates
CC       nucleotide pools across a concentration gradient between both sides of
CC       the mitosome membrane. {ECO:0000269|PubMed:18449191}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=24.2 uM for ATP uptake {ECO:0000269|PubMed:18449191};
CC   -!- SUBCELLULAR LOCATION: Mitosome membrane {ECO:0000269|PubMed:18449191};
CC       Multi-pass membrane protein {ECO:0000269|PubMed:18449191}.
CC   -!- SIMILARITY: Belongs to the ADP/ATP translocase tlc family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; EU040268; ABW20409.1; -; Genomic_DNA.
DR   EMBL; AL590449; CAD25771.1; -; Genomic_DNA.
DR   RefSeq; NP_586167.1; NM_001042000.1.
DR   AlphaFoldDB; Q8SUG0; -.
DR   TCDB; 2.A.12.1.12; the atp:adp antiporter (aaa) family.
DR   GeneID; 859816; -.
DR   KEGG; ecu:ECU10_0520; -.
DR   VEuPathDB; MicrosporidiaDB:ECU10_0520; -.
DR   HOGENOM; CLU_023964_1_0_1; -.
DR   InParanoid; Q8SUG0; -.
DR   OrthoDB; 1270398at2759; -.
DR   Proteomes; UP000000819; Chromosome X.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0032047; C:mitosome; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0005471; F:ATP:ADP antiporter activity; IEA:InterPro.
DR   InterPro; IPR004667; ADP_ATP_car_bac_type.
DR   PANTHER; PTHR31187; PTHR31187; 1.
DR   Pfam; PF03219; TLC; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Membrane; Mitosome; Nucleotide-binding; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..543
FT                   /note="ADP,ATP carrier protein 3"
FT                   /id="PRO_0000382926"
FT   TRANSMEM        46..66
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        86..106
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        111..131
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        175..195
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        209..229
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        243..263
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        306..326
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        346..366
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        382..402
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        504..524
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   543 AA;  61648 MW;  44D97AB5F2A55B1A CRC64;
     MSTFQLSASS KDSYLFRTEE ELEEEVYGKT GFFKHIRVAR NEWPRVLYLS LLFGVITMVH
     TIMGNLREMV LMGRQDPMSM FFIKSIFLPP CSLLFIWAIQ LGLSLFTPSK MFDITLILFS
     GCYILFGLVV WPLKGYIQKD FYWSRDIFGD GKMESLRIHF LYPVFLVFNE WTSSFLFLCS
     EMWGALVVSY FFNIFANEVS TRRQSQRYIS VYNISNAISI FLSAVLTLVF NKWRDGVAFE
     TKELGFRILI LVLGSTVIGI LALKKYMERE ILPAPVFLIR EVEKTSTERR KLKLDEARQT
     LSRSKLLIAI SLNVLLYGVT STLVEATFKS GIAAGARYTN NSKETFANFY NGLEQIIIAI
     SLLVVINTPY SALVKKGGWK YLASLPIVIA MFSLFSVFLI AFYNVGADSG GNVLFGSLFK
     NRMPTFILEN TLGLVTNASM KIGKYLGADV SKEAISMQID PLYRAKYKAV YDGLCGKLGK
     SLGSIICTVM TGLWDITDIR RVSSVSGILI VIIIAMWYFI LKYLSRQFQA AVEANTYIEL
     DEF