ID   NTT4_ENCCU              Reviewed;         536 AA.
AC   Q8SUG7;
DT   01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=ADP,ATP carrier protein 4;
DE   AltName: Full=ADP/ATP translocase 4;
DE   AltName: Full=Nucleotide transporter 4;
GN   Name=NTT4; OrderedLocusNames=ECU10_0420;
OS   Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite).
OC   Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Unikaryonidae;
OC   Encephalitozoon.
OX   NCBI_TaxID=284813;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, FUNCTION, AND
RP   BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=18449191; DOI=10.1038/nature06903;
RA   Tsaousis A.D., Kunji E.R.S., Goldberg A.V., Lucocq J.M., Hirt R.P.,
RA   Embley T.M.;
RT   "A novel route for ATP acquisition by the remnant mitochondria of
RT   Encephalitozoon cuniculi.";
RL   Nature 453:553-556(2008).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GB-M1;
RX   PubMed=11719806; DOI=10.1038/35106579;
RA   Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F.,
RA   Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P.,
RA   Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J.,
RA   Vivares C.P.;
RT   "Genome sequence and gene compaction of the eukaryote parasite
RT   Encephalitozoon cuniculi.";
RL   Nature 414:450-453(2001).
CC   -!- FUNCTION: ATP transporter involved in the uptake of ATP from the host
CC       cell cytoplasm. Provides the microsporidian cell with host ATP in
CC       exchange for ADP. This is an obligate exchange system. This energy
CC       acquiring activity is an important component of microsporidian
CC       parasitism. {ECO:0000269|PubMed:18449191}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=2.6 uM for ATP uptake {ECO:0000269|PubMed:18449191};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:18449191};
CC       Multi-pass membrane protein {ECO:0000269|PubMed:18449191}. Note=Only
CC       found on the surface of parasites living inside host cells.
CC   -!- SIMILARITY: Belongs to the ADP/ATP translocase tlc family.
CC       {ECO:0000305}.
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DR   EMBL; EU040269; ABW20410.1; -; Genomic_DNA.
DR   EMBL; AL590449; CAD25761.1; -; Genomic_DNA.
DR   RefSeq; NP_586157.1; NM_001041990.1.
DR   AlphaFoldDB; Q8SUG7; -.
DR   TCDB; 2.A.12.1.13; the atp:adp antiporter (aaa) family.
DR   GeneID; 859806; -.
DR   KEGG; ecu:ECU10_0420; -.
DR   VEuPathDB; MicrosporidiaDB:ECU10_0420; -.
DR   HOGENOM; CLU_023964_1_0_1; -.
DR   InParanoid; Q8SUG7; -.
DR   OrthoDB; 1270398at2759; -.
DR   Proteomes; UP000000819; Chromosome X.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0005471; F:ATP:ADP antiporter activity; IEA:InterPro.
DR   InterPro; IPR004667; ADP_ATP_car_bac_type.
DR   PANTHER; PTHR31187; PTHR31187; 1.
DR   Pfam; PF03219; TLC; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Cell membrane; Glycoprotein; Membrane; Nucleotide-binding;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..536
FT                   /note="ADP,ATP carrier protein 4"
FT                   /id="PRO_0000382927"
FT   TRANSMEM        44..64
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        77..97
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        109..129
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        172..194
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        205..225
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        244..264
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        309..329
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        349..369
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        378..398
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        465..485
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        493..513
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        400
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        421
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   536 AA;  60856 MW;  667A534F9D5D814A CRC64;
     MSENREIDAT DRRDKTFDKE KLRPHVYSSV AGGMRSTSGD TKAVLLFSLL FALLSYIDAF
     LYVLGDMVMM NTQMPSSILF IKSVLVLPMT FFFIVIVQKG LRYLSQPRML EVILIISSVF
     FLLFGFVIWP YCKRLQPDFF WSRDIFSDGK MKTRHLDFFF PIFLVFSEWA STMLYLVAEL
     WGSLIISFMF FSRAIHQCTE AQVKKFLPTI SLISAVVFLS SGLLTKSLNS RRDALPYHEK
     ERLFSQVFIV TSALTVMSAI TSFFTDRALA KDDPRHKGKK EHKVRKIGFA GSLKMMQKSR
     FLRAMTESVV AASVCSNIFE AIYRGGIVLG AVQSSTSKSS YMNRLNAMAQ IITSIFLLVM
     FFKPATHLIE RRGWFPVAIT APIVAIITLV LFFPMVFFNN ITEGDLIASG EEYVGSFVLE
     NYTGMFLTTI IRISKYCFFD VAKEAASIRV SPVHRHSFRG IHDGLGINIG KTIGSVYCTL
     VTVVFDVRDV RNVVSVSTVF VGVFCVIWIR SILHINKKYK ESIERNDFIN VELAEG