NU1M_DROME
ID NU1M_DROME Reviewed; 312 AA.
AC P18929;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2017, sequence version 3.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 1;
DE EC=7.1.1.2;
DE AltName: Full=NADH dehydrogenase subunit 1;
GN Name=mt:ND1; Synonyms=ND1;
OS Drosophila melanogaster (Fruit fly).
OG Mitochondrion.
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Bretagne;
RX PubMed=3130291; DOI=10.1093/genetics/118.4.649;
RA Garesse R.;
RT "Drosophila melanogaster mitochondrial DNA: gene organization and
RT evolutionary considerations.";
RL Genetics 118:649-663(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=8825764; DOI=10.1111/j.1365-2583.1995.tb00032.x;
RA Lewis D.L., Farr C.L., Kaguni L.S.;
RT "Drosophila melanogaster mitochondrial DNA: completion of the nucleotide
RT sequence and evolutionary comparisons.";
RL Insect Mol. Biol. 4:263-278(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RA Wan K., Celniker S.;
RL Submitted (AUG-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the complex I subunit 1 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M37275; AAA69715.1; -; Genomic_DNA.
DR EMBL; U37541; AAC47823.1; -; Genomic_DNA.
DR EMBL; KJ947872; AIC64016.1; -; Genomic_DNA.
DR PIR; S01191; S01191.
DR RefSeq; YP_009047278.1; NC_024511.2.
DR AlphaFoldDB; P18929; -.
DR SMR; P18929; -.
DR STRING; 7227.FBpp0100187; -.
DR PaxDb; P18929; -.
DR EnsemblMetazoa; FBtr0433499; FBpp0390631; FBgn0013679.
DR GeneID; 19893558; -.
DR KEGG; dme:Dmel_CG34092; -.
DR CTD; 4535; -.
DR FlyBase; FBgn0013679; mt:ND1.
DR VEuPathDB; VectorBase:FBgn0013679; -.
DR eggNOG; KOG4770; Eukaryota.
DR HOGENOM; CLU_015134_0_1_1; -.
DR InParanoid; P18929; -.
DR OrthoDB; 1214989at2759; -.
DR PhylomeDB; P18929; -.
DR Reactome; R-DME-611105; Respiratory electron transport.
DR Reactome; R-DME-6799198; Complex I biogenesis.
DR BioGRID-ORCS; 19893558; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 19893558; -.
DR PRO; PR:P18929; -.
DR Proteomes; UP000000803; Mitochondrion.
DR Bgee; FBgn0013679; Expressed in Malpighian tubule and 14 other tissues.
DR ExpressionAtlas; P18929; baseline and differential.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; ISS:FlyBase.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR GO; GO:0009060; P:aerobic respiration; IBA:GO_Central.
DR HAMAP; MF_01350; NDH1_NuoH; 1.
DR InterPro; IPR001694; NADH_UbQ_OxRdtase_su1/FPO.
DR InterPro; IPR018086; NADH_UbQ_OxRdtase_su1_CS.
DR PANTHER; PTHR11432; PTHR11432; 1.
DR Pfam; PF00146; NADHdh; 1.
DR PROSITE; PS00667; COMPLEX1_ND1_1; 1.
DR PROSITE; PS00668; COMPLEX1_ND1_2; 1.
PE 3: Inferred from homology;
KW Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW NAD; Reference proteome; Respiratory chain; Translocase; Transmembrane;
KW Transmembrane helix; Transport; Ubiquinone.
FT CHAIN 1..312
FT /note="NADH-ubiquinone oxidoreductase chain 1"
FT /id="PRO_0000117392"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..171
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 174..194
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 226..246
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 250..270
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 289..309
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 190
FT /note="M -> V (in Ref. 1; AAA69715 and 2; AAC47823)"
FT /evidence="ECO:0000305"
FT CONFLICT 200
FT /note="T -> N (in Ref. 1; AAA69715 and 2; AAC47823)"
FT /evidence="ECO:0000305"
FT CONFLICT 224
FT /note="F -> L (in Ref. 1; AAA69715 and 2; AAC47823)"
FT /evidence="ECO:0000305"
FT CONFLICT 248
FT /note="G -> P (in Ref. 1; AAA69715 and 2; AAC47823)"
FT /evidence="ECO:0000305"
FT CONFLICT 256
FT /note="F -> I (in Ref. 1; AAA69715 and 2; AAC47823)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 312 AA; 35957 MW; F78D03B04B4BEA3E CRC64;
MEFILSLIGS LLLIICVLVS VAFLTLLERK VLGYIQIRKG PNKVGLMGIP QPFCDAIKLF
TKEQTYPLLS NYLSYYISPI FSLFLSLFVW MCMPFFVKLY SFNLGGLFFL CCTSLGVYTV
MVAGWSSNSN YALLGGLRAV AQTISYEVSL ALILLSFIFL IGSYNMIYFF FYQVYMWFLI
ILFPMALVWM SISLAETNRT PFDFAEGESE LVSGFNVEYS SGGFALIFMA EYASILFMSM
LFCVIFLGCD VFNLLFYMKL TFISFVFIWV RGTLPRFRYD KLMYLAWKCF LSFSLNYLLF
FIGFKILLFS LL
