ID   NU1M_LITCT              Reviewed;         324 AA.
AC   P16672; Q6LDK1;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   26-APR-2005, sequence version 3.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 1;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 1;
GN   Name=MT-ND1; Synonyms=MTND1, NADH1, ND1;
OS   Lithobates catesbeianus (American bullfrog) (Rana catesbeiana).
OG   Mitochondrion.
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Lithobates.
OX   NCBI_TaxID=8400;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3263985; DOI=10.1272/jnms1923.55.417;
RA   Nagae Y.;
RT   "The primary and secondary structures of the ribosomal RNAs of Rana
RT   catesbeiana mitochondrion.";
RL   Nippon Ika Daigaku Zasshi 55:417-430(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 256-320.
RX   PubMed=3497168; DOI=10.1272/jnms1923.54.277;
RA   Fujii H.;
RT   "Cloning of the entire mitochondrial genome of Rana catesbeiana and
RT   nucleotide sequencing of the URF2 and its flanking genes.";
RL   Nippon Ika Daigaku Zasshi 54:277-289(1987).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 256-320.
RX   PubMed=3260590; DOI=10.1093/oxfordjournals.jbchem.a122295;
RA   Fujii H., Shimada T., Goto Y., Okazaki T.;
RT   "Cloning of the mitochondrial genome of Rana catesbeiana and the nucleotide
RT   sequences of the ND2 and five tRNA genes.";
RL   J. Biochem. 103:474-481(1988).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Multi-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 1 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M57527; AAA65487.3; -; Genomic_DNA.
DR   EMBL; D10368; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; A30478; A30478.
DR   AlphaFoldDB; P16672; -.
DR   SMR; P16672; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   HAMAP; MF_01350; NDH1_NuoH; 1.
DR   InterPro; IPR001694; NADH_UbQ_OxRdtase_su1/FPO.
DR   InterPro; IPR018086; NADH_UbQ_OxRdtase_su1_CS.
DR   PANTHER; PTHR11432; PTHR11432; 1.
DR   Pfam; PF00146; NADHdh; 1.
DR   PROSITE; PS00667; COMPLEX1_ND1_1; 1.
DR   PROSITE; PS00668; COMPLEX1_ND1_2; 1.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   NAD; Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW   Transport; Ubiquinone.
FT   CHAIN           1..324
FT                   /note="NADH-ubiquinone oxidoreductase chain 1"
FT                   /id="PRO_0000117467"
FT   TRANSMEM        3..23
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        73..93
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        106..126
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        151..171
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        175..195
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        226..246
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        255..275
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        295..315
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   324 AA;  36147 MW;  7CDE21B3AA447B04 CRC64;
     MKLLFMYLLP LLYIAPILLA VAFLTLIERK ILGYMQHRKG PNVVGPFGLL QPIADGVKLF
     IKEPIRPSTS SQLLFILAPT LALALAMIMW TPFPLPIPYS NLNLSILFIL AISSLTVYTI
     LGSGWASNSK YALIGALRAV AQTISYEVTL ALIILCSVLL AGGFTLSAFA ISQEFTWFIL
     PLWPMFLMWF VSTLAETNRA PFDLTEGESE LVSGFNVEYA GGPFALFFLA EYANILMMNT
     LSTIIFLGSC MSSLTLTTSL LMTKASILSL CFLWVRASYP RFRYDQLMHL VWKNFLPLTL
     ALVILYVSMP ISLLFTPPLP WKRA