NU1M_MOUSE
ID NU1M_MOUSE Reviewed; 318 AA.
AC P03888; Q9G0S6;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 04-JAN-2005, sequence version 3.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 1;
DE EC=7.1.1.2 {ECO:0000250|UniProtKB:P03886};
DE AltName: Full=NADH dehydrogenase subunit 1;
GN Name=Mtnd1; Synonyms=mt-Nd1, Nd1;
OS Mus musculus (Mouse).
OG Mitochondrion.
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7332926; DOI=10.1016/0092-8674(81)90300-7;
RA Bibb M.J., van Etten R.A., Wright C.T., Walberg M.W., Clayton D.A.;
RT "Sequence and gene organization of mouse mitochondrial DNA.";
RL Cell 26:167-180(1981).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=C3H/He, SAMP1, SAMP8, and SAMR1; TISSUE=Liver;
RX PubMed=11535650; DOI=10.1093/jhered/92.4.352;
RA Mizutani J., Chiba T., Tanaka M., Higuchi K., Mori M.;
RT "Unique mutations in mitochondrial DNA of senescence-accelerated mouse
RT (SAM) strains.";
RL J. Hered. 92:352-355(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ALR/Lt, ALS/Lt, NOD/LtJ, and NON/Lt;
RA Mathews C.E., Leiter E.H., Spirina O., Bykhovskaya Y., Fischel-Ghodsian N.;
RT "The ALR/Lt mouse: contribution of the mitochondrial genome in resistance
RT against both chemically-induced and autoimmune diabetes.";
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BALB/cJ, C3H/An, and C57BL/6J; TISSUE=Fibroblast, and Platelet;
RX PubMed=12954771; DOI=10.1093/nar/gkg739;
RA Bayona-Bafaluy M.P., Acin-Perez R., Mullikin J.C., Park J.S.,
RA Moreno-Loshuertos R., Hu P., Perez-Martos A., Fernandez-Silva P., Bai Y.,
RA Enriquez J.A.;
RT "Revisiting the mouse mitochondrial DNA sequence.";
RL Nucleic Acids Res. 31:5349-5355(2003).
RN [5]
RP PROTEIN SEQUENCE OF 27-54 AND 127-134, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RC STRAIN=C57BL/6J; TISSUE=Brain;
RA Lubec G., Kang S.U.;
RL Submitted (APR-2007) to UniProtKB.
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) which catalyzes electron transfer from
CC NADH through the respiratory chain, using ubiquinone as an electron
CC acceptor. Essential for the catalytic activity and assembly of complex
CC I. {ECO:0000250|UniProtKB:P03886}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC Evidence={ECO:0000250|UniProtKB:P03886};
CC -!- SUBUNIT: Core subunit of respiratory chain NADH dehydrogenase (Complex
CC I) which is composed of 45 different subunits.
CC {ECO:0000250|UniProtKB:P03887}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:P03887}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the complex I subunit 1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB48644.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAA24080.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; V00711; CAA24080.1; ALT_INIT; Genomic_DNA.
DR EMBL; J01420; AAB48644.1; ALT_INIT; Genomic_DNA.
DR EMBL; AB042523; BAA95653.2; -; Genomic_DNA.
DR EMBL; AB042524; BAA95640.2; -; Genomic_DNA.
DR EMBL; AB042809; BAA95795.2; -; Genomic_DNA.
DR EMBL; AB049357; BAB13801.2; -; Genomic_DNA.
DR EMBL; AJ512208; CAD54432.1; -; Genomic_DNA.
DR EMBL; AY172335; AAN85122.1; -; Genomic_DNA.
DR EMBL; AY339599; AAP89023.2; -; Genomic_DNA.
DR EMBL; AJ489607; CAD33907.1; -; Genomic_DNA.
DR EMBL; AY533105; AAS01439.1; -; Genomic_DNA.
DR EMBL; AY533106; AAS01452.1; -; Genomic_DNA.
DR EMBL; AY533107; AAS01465.1; -; Genomic_DNA.
DR EMBL; AY533108; AAS01478.1; -; Genomic_DNA.
DR PIR; A00409; QXMS1M.
DR RefSeq; NP_904328.1; NC_005089.1.
DR RefSeq; YP_220550.1; NC_006914.1.
DR PDB; 6G2J; EM; 3.30 A; H=1-318.
DR PDB; 6G72; EM; 3.90 A; H=1-318.
DR PDB; 6ZR2; EM; 3.10 A; H=1-318.
DR PDB; 6ZTQ; EM; 3.00 A; H=1-318.
DR PDB; 7AK5; EM; 3.17 A; H=1-318.
DR PDB; 7AK6; EM; 3.82 A; H=1-318.
DR PDB; 7B93; EM; 3.04 A; H=1-318.
DR PDB; 7LFI; X-ray; 1.70 A; C/F=1-7.
DR PDB; 7LFJ; X-ray; 1.70 A; C/F=1-7.
DR PDB; 7LFK; X-ray; 1.60 A; C/F=1-7.
DR PDB; 7LFL; X-ray; 1.60 A; C=1-7.
DR PDB; 7LFM; X-ray; 1.60 A; C/F=1-7.
DR PDB; 7PSA; EM; 3.40 A; H=1-318.
DR PDBsum; 6G2J; -.
DR PDBsum; 6G72; -.
DR PDBsum; 6ZR2; -.
DR PDBsum; 6ZTQ; -.
DR PDBsum; 7AK5; -.
DR PDBsum; 7AK6; -.
DR PDBsum; 7B93; -.
DR PDBsum; 7LFI; -.
DR PDBsum; 7LFJ; -.
DR PDBsum; 7LFK; -.
DR PDBsum; 7LFL; -.
DR PDBsum; 7LFM; -.
DR PDBsum; 7PSA; -.
DR AlphaFoldDB; P03888; -.
DR SMR; P03888; -.
DR BioGRID; 201547; 5.
DR ComplexPortal; CPX-266; Mitochondrial respiratory chain complex I.
DR CORUM; P03888; -.
DR DIP; DIP-61655N; -.
DR IntAct; P03888; 4.
DR STRING; 10090.ENSMUSP00000080991; -.
DR EPD; P03888; -.
DR jPOST; P03888; -.
DR PaxDb; P03888; -.
DR PRIDE; P03888; -.
DR ProteomicsDB; 253037; -.
DR Antibodypedia; 35355; 237 antibodies from 27 providers.
DR Ensembl; ENSMUST00000082392; ENSMUSP00000080991; ENSMUSG00000064341.
DR GeneID; 17716; -.
DR GeneID; 3338902; -.
DR KEGG; mmu:17716; -.
DR CTD; 4535; -.
DR MGI; MGI:101787; mt-Nd1.
DR VEuPathDB; HostDB:ENSMUSG00000064341; -.
DR eggNOG; KOG4770; Eukaryota.
DR GeneTree; ENSGT00390000006621; -.
DR HOGENOM; CLU_015134_0_1_1; -.
DR InParanoid; P03888; -.
DR OMA; WSGWASN; -.
DR OrthoDB; 1214989at2759; -.
DR PhylomeDB; P03888; -.
DR Reactome; R-MMU-611105; Respiratory electron transport.
DR Reactome; R-MMU-6799198; Complex I biogenesis.
DR ChiTaRS; mt-Nd1; mouse.
DR PRO; PR:P03888; -.
DR Proteomes; UP000000589; Mitochondrion.
DR RNAct; P03888; protein.
DR Bgee; ENSMUSG00000064341; Expressed in embryonic post-anal tail and 60 other tissues.
DR ExpressionAtlas; P03888; baseline and differential.
DR GO; GO:0030425; C:dendrite; ISO:MGI.
DR GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR GO; GO:0031966; C:mitochondrial membrane; ISO:MGI.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; ISO:MGI.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0043025; C:neuronal cell body; ISO:MGI.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; ISS:UniProtKB.
DR GO; GO:0009060; P:aerobic respiration; IBA:GO_Central.
DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; ISS:UniProtKB.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR GO; GO:0042776; P:proton motive force-driven mitochondrial ATP synthesis; IC:ComplexPortal.
DR GO; GO:0033194; P:response to hydroperoxide; ISO:MGI.
DR GO; GO:0014070; P:response to organic cyclic compound; IEA:Ensembl.
DR GO; GO:0009410; P:response to xenobiotic stimulus; IEA:Ensembl.
DR HAMAP; MF_01350; NDH1_NuoH; 1.
DR InterPro; IPR001694; NADH_UbQ_OxRdtase_su1/FPO.
DR InterPro; IPR018086; NADH_UbQ_OxRdtase_su1_CS.
DR PANTHER; PTHR11432; PTHR11432; 1.
DR Pfam; PF00146; NADHdh; 1.
DR PROSITE; PS00667; COMPLEX1_ND1_1; 1.
DR PROSITE; PS00668; COMPLEX1_ND1_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Electron transport; Membrane;
KW Mitochondrion; Mitochondrion inner membrane; NAD; Reference proteome;
KW Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW Transport; Ubiquinone.
FT CHAIN 1..318
FT /note="NADH-ubiquinone oxidoreductase chain 1"
FT /id="PRO_0000117431"
FT TRANSMEM 2..22
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 70..90
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 231..251
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 253..273
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 293..313
FT /note="Helical"
FT /evidence="ECO:0000255"
FT HELIX 2..31
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 39..46
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 47..57
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 64..66
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 68..84
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 85..88
FT /evidence="ECO:0007829|PDB:6ZR2"
FT STRAND 91..93
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 101..122
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 126..154
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 155..157
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 158..160
FT /evidence="ECO:0007829|PDB:6ZR2"
FT HELIX 164..168
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 170..172
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 175..177
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 180..191
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 192..194
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 196..198
FT /evidence="ECO:0007829|PDB:6G2J"
FT TURN 199..202
FT /evidence="ECO:0007829|PDB:7B93"
FT TURN 204..206
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 207..209
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 210..214
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 218..241
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 252..276
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 281..283
FT /evidence="ECO:0007829|PDB:6G2J"
FT HELIX 284..291
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 293..310
FT /evidence="ECO:0007829|PDB:6ZTQ"
SQ SEQUENCE 318 AA; 36059 MW; ECFAB96696610024 CRC64;
MFFINILTLL VPILIAMAFL TLVERKILGY MQLRKGPNIV GPYGILQPFA DAMKLFMKEP
MRPLTTSMSL FIIAPTLSLT LALSLWVPLP MPHPLINLNL GILFILATSS LSVYSILWSG
WASNSKYSLF GALRAVAQTI SYEVTMAIIL LSVLLMNGSY SLQTLITTQE HMWLLLPAWP
MAMMWFISTL AETNRAPFDL TEGESELVSG FNVEYAAGPF ALFFMAEYTN IILMNALTTI
IFLGPLYYIN LPELYSTNFM MEALLLSSTF LWIRASYPRF RYDQLMHLLW KNFLPLTLAL
CMWHISLPIF TAGVPPYM
