ID   NU1M_WICCA              Reviewed;         351 AA.
AC   P48901;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   30-AUG-2002, sequence version 2.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 1;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 1;
GN   Name=ND1;
OS   Wickerhamomyces canadensis (Yeast) (Pichia canadensis).
OG   Mitochondrion.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Phaffomycetaceae; Wickerhamomyces.
OX   NCBI_TaxID=1156965;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=21;
RX   PubMed=8536312; DOI=10.1007/bf00311880;
RA   Sekito T., Okamoto K., Kitano H., Yoshida K.;
RT   "The complete mitochondrial DNA sequence of Hansenula wingei reveals new
RT   characteristics of yeast mitochondria.";
RL   Curr. Genet. 28:39-53(1995).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Multi-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 1 family. {ECO:0000305}.
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DR   EMBL; D31785; BAA06568.2; -; Genomic_DNA.
DR   PIR; S58745; S58745.
DR   RefSeq; NP_038213.1; NC_001762.1.
DR   AlphaFoldDB; P48901; -.
DR   SMR; P48901; -.
DR   GeneID; 800553; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   HAMAP; MF_01350; NDH1_NuoH; 1.
DR   InterPro; IPR001694; NADH_UbQ_OxRdtase_su1/FPO.
DR   InterPro; IPR018086; NADH_UbQ_OxRdtase_su1_CS.
DR   PANTHER; PTHR11432; PTHR11432; 1.
DR   Pfam; PF00146; NADHdh; 1.
DR   PROSITE; PS00667; COMPLEX1_ND1_1; 1.
DR   PROSITE; PS00668; COMPLEX1_ND1_2; 1.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   NAD; Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW   Transport; Ubiquinone.
FT   CHAIN           1..351
FT                   /note="NADH-ubiquinone oxidoreductase chain 1"
FT                   /id="PRO_0000117456"
FT   TRANSMEM        1..21
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        46..66
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        72..92
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        106..126
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        141..161
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        176..196
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        238..258
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        279..299
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        324..344
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   351 AA;  40151 MW;  0A552C16F3DC6765 CRC64;
     MIILNIVEMI VLFIFILLSV AYLTVAERKT LGYMQRRVGP NIVGYYGQLM AIADALKLLL
     KEIIIVRESD KILIIISPII ALCFALLSWA VIPFSPGIAI GDMNYSLLYI LVIGSIGVFG
     TLLVGWSSNS KFTLLASIRS TAQLISYELI LTTNIIICIL LNNTLNLNSF IDNQQTIWLI
     LPLLPIGIIH YIARIAETSR PPFDNVEAES ELVSGHMTEL SASPFVLFYL QEYNNIVIMC
     FLNIILFFGG YLFWLPFIDN GSNLINFIIN YFNINNFNFI YIIEIIFYVI NISFKSIILT
     YGFIWVRASF PRLRYDLLVN LCWVIFLPLL FSLLILIPHI LYIFDSFNIY N