NU2C2_ANTAG
ID NU2C2_ANTAG Reviewed; 500 AA.
AC P0CC31; Q85CP2;
DT 09-FEB-2010, integrated into UniProtKB/Swiss-Prot.
DT 09-FEB-2010, sequence version 1.
DT 25-MAY-2022, entry version 30.
DE RecName: Full=NAD(P)H-quinone oxidoreductase subunit 2 B, chloroplastic {ECO:0000255|HAMAP-Rule:MF_00445};
DE EC=7.1.1.- {ECO:0000255|HAMAP-Rule:MF_00445};
DE AltName: Full=NAD(P)H dehydrogenase, subunit 2 B {ECO:0000255|HAMAP-Rule:MF_00445};
DE AltName: Full=NADH-plastoquinone oxidoreductase subunit 2 B {ECO:0000255|HAMAP-Rule:MF_00445};
GN Name=ndhB2 {ECO:0000255|HAMAP-Rule:MF_00445};
OS Anthoceros angustus (Hornwort) (Anthoceros formosae).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Anthocerotophyta;
OC Anthocerotopsida; Anthocerotidae; Anthocerotales; Anthocerotaceae;
OC Anthoceros.
OX NCBI_TaxID=48387;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND RNA EDITING.
RX PubMed=12527781; DOI=10.1093/nar/gkg155;
RA Kugita M., Kaneko A., Yamamoto Y., Takeya Y., Matsumoto T., Yoshinaga K.;
RT "The complete nucleotide sequence of the hornwort (Anthoceros formosae)
RT chloroplast genome: insight into the earliest land plants.";
RL Nucleic Acids Res. 31:716-721(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND RNA EDITING.
RC TISSUE=Thallus;
RX PubMed=12711687; DOI=10.1093/nar/gkg327;
RA Kugita M., Yamamoto Y., Fujikawa T., Matsumoto T., Yoshinaga K.;
RT "RNA editing in hornwort chloroplasts makes more than half the genes
RT functional.";
RL Nucleic Acids Res. 31:2417-2423(2003).
CC -!- FUNCTION: NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN
CC and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain
CC and possibly in a chloroplast respiratory chain. The immediate electron
CC acceptor for the enzyme in this species is believed to be
CC plastoquinone. Couples the redox reaction to proton translocation, and
CC thus conserves the redox energy in a proton gradient.
CC {ECO:0000255|HAMAP-Rule:MF_00445}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a plastoquinone + (n+1) H(+)(in) + NADH = a plastoquinol + n
CC H(+)(out) + NAD(+); Xref=Rhea:RHEA:42608, Rhea:RHEA-COMP:9561,
CC Rhea:RHEA-COMP:9562, ChEBI:CHEBI:15378, ChEBI:CHEBI:17757,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:62192;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00445};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a plastoquinone + (n+1) H(+)(in) + NADPH = a plastoquinol + n
CC H(+)(out) + NADP(+); Xref=Rhea:RHEA:42612, Rhea:RHEA-COMP:9561,
CC Rhea:RHEA-COMP:9562, ChEBI:CHEBI:15378, ChEBI:CHEBI:17757,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:62192;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00445};
CC -!- SUBUNIT: NDH is composed of at least 16 different subunits, 5 of which
CC are encoded in the nucleus. {ECO:0000255|HAMAP-Rule:MF_00445}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000255|HAMAP-Rule:MF_00445}; Multi-pass membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_00445}.
CC -!- RNA EDITING: Modified_positions=89 {ECO:0000269|PubMed:12527781,
CC ECO:0000269|PubMed:12711687}, 120 {ECO:0000269|PubMed:12527781,
CC ECO:0000269|PubMed:12711687}, 145 {ECO:0000269|PubMed:12527781,
CC ECO:0000269|PubMed:12711687}, 157 {ECO:0000269|PubMed:12527781,
CC ECO:0000269|PubMed:12711687}, 158 {ECO:0000269|PubMed:12527781,
CC ECO:0000269|PubMed:12711687}, 167 {ECO:0000269|PubMed:12527781,
CC ECO:0000269|PubMed:12711687}, 174 {ECO:0000269|PubMed:12527781,
CC ECO:0000269|PubMed:12711687}, 180 {ECO:0000269|PubMed:12527781,
CC ECO:0000269|PubMed:12711687}, 197 {ECO:0000269|PubMed:12527781,
CC ECO:0000269|PubMed:12711687}, 294 {ECO:0000269|PubMed:12527781,
CC ECO:0000269|PubMed:12711687}, 310 {ECO:0000269|PubMed:12527781,
CC ECO:0000269|PubMed:12711687}, 335 {ECO:0000269|PubMed:12527781,
CC ECO:0000269|PubMed:12711687}, 341 {ECO:0000269|PubMed:12527781,
CC ECO:0000269|PubMed:12711687}, 368 {ECO:0000269|PubMed:12527781,
CC ECO:0000269|PubMed:12711687}, 373 {ECO:0000269|PubMed:12527781,
CC ECO:0000269|PubMed:12711687}, 379 {ECO:0000269|PubMed:12527781,
CC ECO:0000269|PubMed:12711687}, 399 {ECO:0000269|PubMed:12527781,
CC ECO:0000269|PubMed:12711687}, 402 {ECO:0000269|PubMed:12527781,
CC ECO:0000269|PubMed:12711687}, 454 {ECO:0000269|PubMed:12527781,
CC ECO:0000269|PubMed:12711687}, 476 {ECO:0000269|PubMed:12527781,
CC ECO:0000269|PubMed:12711687}; Note=The nonsense codon at position 157
CC is modified to a sense codon.;
CC -!- SIMILARITY: Belongs to the complex I subunit 2 family.
CC {ECO:0000255|HAMAP-Rule:MF_00445}.
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DR EMBL; AB086179; BAC55413.1; -; Genomic_DNA.
DR RefSeq; NP_777457.1; NC_004543.1.
DR RefSeq; NP_777476.1; NC_004543.1.
DR AlphaFoldDB; P0CC31; -.
DR SMR; P0CC31; -.
DR GeneID; 2553494; -.
DR GeneID; 2553495; -.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:InterPro.
DR GO; GO:0048038; F:quinone binding; IEA:UniProtKB-KW.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR GO; GO:0019684; P:photosynthesis, light reaction; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00445; NDH1_NuoN_1; 1.
DR InterPro; IPR010096; NADH-Q_OxRdtase_suN/2.
DR InterPro; IPR001750; ND/Mrp_mem.
DR InterPro; IPR045693; Ndh2_N.
DR Pfam; PF19530; Ndh2_N; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR TIGRFAMs; TIGR01770; NDH_I_N; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Membrane; NAD; NADP; Plastid; Plastoquinone; Quinone;
KW RNA editing; Thylakoid; Translocase; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..500
FT /note="NAD(P)H-quinone oxidoreductase subunit 2 B,
FT chloroplastic"
FT /id="PRO_0000391253"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00445"
FT TRANSMEM 41..61
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00445"
FT TRANSMEM 78..98
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00445"
FT TRANSMEM 116..136
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00445"
FT TRANSMEM 166..186
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00445"
FT TRANSMEM 211..231
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00445"
FT TRANSMEM 242..262
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00445"
FT TRANSMEM 277..297
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00445"
FT TRANSMEM 305..325
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00445"
FT TRANSMEM 335..355
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00445"
FT TRANSMEM 376..396
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00445"
FT TRANSMEM 409..429
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00445"
FT TRANSMEM 467..487
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00445"
SQ SEQUENCE 500 AA; 55243 MW; 0ED218E87D474C86 CRC64;
MKLDFGSFLS DGSSILPECI LISSLIIILL IDLTSEKKTY WLYFISLTSL IISITVLLFQ
LKEEPIFSFS GSFQTDGFNG IFRISIAFSS LLCIPLSMEY MKCTKMAITE SLIFLLTATI
GGMFLCGAND LIIIFITLEC LSLSSYLLSG YTKKDVRSNE AAMKYLLMGG ASSSILAYGF
SWLYGLSGGK IQLQEIFNGL INTQMYNSTS ISIVLIFIIA GIAFKLSLVP FHQWTPDVYE
GAPTSVIAFF SVTSKIAGLA LATRIFNTVF FSSLNEWHLI LEIIAILSMI LGNFIAITQT
SMKRMLAYSS ISQIGYFMIG VIAGDSNGYA SMITYMLFYI FMNLGTFACI TLFGLRTGTD
NIRDYAGLYK KDPLLASFLA LSLLSLGGIP PLAGFFGKLY LFWCGWKAGL YLSVSVGLFT
SVISIYYYLR IVKLIVTKEN EETTSYIRKY KTSSNYLVSK SPIEFSIIIC VIGSTFSGIV
INPVIAIVEK TISLSSFINN
