ID   NU2M_ACACA              Reviewed;         527 AA.
AC   Q37376;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 2;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 2;
GN   Name=ND2; Synonyms=NAD2;
OS   Acanthamoeba castellanii (Amoeba).
OG   Mitochondrion.
OC   Eukaryota; Amoebozoa; Discosea; Longamoebia; Centramoebida;
OC   Acanthamoebidae; Acanthamoeba.
OX   NCBI_TaxID=5755;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 30010 / Neff;
RX   PubMed=7844823; DOI=10.1006/jmbi.1994.0043;
RA   Burger G., Plante I., Lonergan K.M., Gray M.W.;
RT   "The mitochondrial DNA of the amoeboid protozoon, Acanthamoeba castellanii:
RT   complete sequence, gene content and genome organization.";
RL   J. Mol. Biol. 245:522-537(1995).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane; Multi-pass membrane
CC       protein.
CC   -!- SIMILARITY: Belongs to the complex I subunit 2 family. {ECO:0000305}.
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DR   EMBL; U12386; AAD11827.1; -; Genomic_DNA.
DR   PIR; S53835; S53835.
DR   RefSeq; NP_042534.1; NC_001637.1.
DR   AlphaFoldDB; Q37376; -.
DR   SMR; Q37376; -.
DR   GeneID; 1734029; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR   HAMAP; MF_00445; NDH1_NuoN_1; 1.
DR   InterPro; IPR010096; NADH-Q_OxRdtase_suN/2.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   TIGRFAMs; TIGR01770; NDH_I_N; 1.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   NAD; Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW   Transport; Ubiquinone.
FT   CHAIN           1..527
FT                   /note="NADH-ubiquinone oxidoreductase chain 2"
FT                   /id="PRO_0000117537"
FT   TRANSMEM        33..53
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        66..86
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        106..126
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        135..155
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        160..180
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        195..215
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        241..261
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        281..303
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        317..337
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        347..367
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        368..388
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        415..435
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        452..472
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        489..509
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   527 AA;  61407 MW;  417DABB22EE04F19 CRC64;
     MNVINFIYSI FDKKLFKCGT ISSRGLFYND LNFFHFEIYF LLALITFLIF FVILSNKKNY
     RSYYYLNVGG IFSNLLPFVV ILLLIILNTN TNDSYYLFAG FYYNDAAVVF FKNIILIGFL
     IFTFAIKQYL SYFKYYDFEF ILVLFISLFS SLLILNSNDL ISLFFIIELQ SLTFYILVAS
     KQTSSFSTES GLKYFILGCF SSGIILFGIS LIYGFTGLLS YTDLTLFLSE VYVTNFILDS
     SFFSFSGFLI GFLLLTVGFL FKLGSAPFHM WMPDVYEGSP LLITAYLSTL PKISLIFVIF
     KLYYYVFFEV FLFSQGLFTL TALFSILLGS IAAIYQVKLK RMLTYSMITN TGYLLLGLSF
     GDISGIYITI FYLISYIFIM IGLFFCFLSL RDRSSGLLVK RINLFSNLLE VNPSLSFSIF
     ILLFSIAGIP PLLGFYSKFF LFLFSLKYKM YWMTILFVIF SVVSVFYYIR LVKLMYFNRT
     TGWIFLYDIP FLNSLIISFI TIINCLFFFN PNLLFKLIYN FSFYLYI