NU2M_APILI
ID NU2M_APILI Reviewed; 333 AA.
AC P34849; P92496; P92497; P92502; P92886; P92907; Q33785; Q33786; Q33787;
AC Q33788; Q36885;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 25-MAY-2022, entry version 82.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 2;
DE EC=7.1.1.2;
DE AltName: Full=NADH dehydrogenase subunit 2;
GN Name=ND2;
OS Apis mellifera ligustica (Common honeybee) (Italian honeybee).
OG Mitochondrion.
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae;
OC Apis.
OX NCBI_TaxID=7469;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TISSUE=Thorax;
RX PubMed=8417993; DOI=10.1093/genetics/133.1.97;
RA Crozier R.H., Crozier Y.C.;
RT "The mitochondrial genome of the honeybee Apis mellifera: complete sequence
RT and genome organization.";
RL Genetics 133:97-117(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-211.
RC STRAIN=Various strains;
RX PubMed=8744768; DOI=10.1006/mpev.1996.0050;
RA Arias M.C., Sheppard W.S.;
RT "Molecular phylogenetics of honey bee subspecies (Apis mellifera L.)
RT inferred from mitochondrial DNA sequence.";
RL Mol. Phylogenet. Evol. 5:557-566(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 60-307.
RC STRAIN=Haplotypes 1 to 11;
RA Koulianos S., Crozier R.H.;
RL Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane; Multi-pass membrane
CC protein.
CC -!- SIMILARITY: Belongs to the complex I subunit 2 family. {ECO:0000305}.
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DR EMBL; L06178; AAB96798.1; -; Genomic_DNA.
DR EMBL; U35743; AAB38223.1; -; Genomic_DNA.
DR EMBL; U35744; AAB38224.1; -; Genomic_DNA.
DR EMBL; U35745; AAB38225.1; -; Genomic_DNA.
DR EMBL; U35746; AAB38226.1; -; Genomic_DNA.
DR EMBL; U35747; AAB38227.1; -; Genomic_DNA.
DR EMBL; U35748; AAD12744.1; -; Genomic_DNA.
DR EMBL; U35749; AAD12745.1; -; Genomic_DNA.
DR EMBL; U35750; AAB38228.1; -; Genomic_DNA.
DR EMBL; U35751; AAB38229.1; -; Genomic_DNA.
DR EMBL; U35752; AAB38230.1; -; Genomic_DNA.
DR EMBL; U35753; AAB38231.1; -; Genomic_DNA.
DR EMBL; U35754; AAB38232.1; -; Genomic_DNA.
DR EMBL; U35755; AAB38233.1; -; Genomic_DNA.
DR EMBL; U35756; AAB38234.1; -; Genomic_DNA.
DR EMBL; U35757; AAB38235.1; -; Genomic_DNA.
DR EMBL; U35758; AAB38236.1; -; Genomic_DNA.
DR EMBL; U35759; AAB38237.1; -; Genomic_DNA.
DR EMBL; U35760; AAB38238.1; -; Genomic_DNA.
DR EMBL; U35761; AAB38239.1; -; Genomic_DNA.
DR EMBL; U35762; AAB38241.1; -; Genomic_DNA.
DR EMBL; U35763; AAB38242.1; -; Genomic_DNA.
DR EMBL; U35764; AAB38243.1; -; Genomic_DNA.
DR EMBL; U35765; AAB38244.1; -; Genomic_DNA.
DR EMBL; U72279; AAB41177.1; -; Genomic_DNA.
DR EMBL; U72280; AAB41178.1; -; Genomic_DNA.
DR EMBL; U72281; AAB41179.1; -; Genomic_DNA.
DR EMBL; U72282; AAB41180.1; -; Genomic_DNA.
DR EMBL; U72283; AAB41181.1; -; Genomic_DNA.
DR EMBL; U72284; AAB41182.1; -; Genomic_DNA.
DR EMBL; U72285; AAB41183.1; -; Genomic_DNA.
DR EMBL; U72286; AAB41184.1; -; Genomic_DNA.
DR EMBL; U72287; AAB41185.1; -; Genomic_DNA.
DR EMBL; U72288; AAB41186.1; -; Genomic_DNA.
DR EMBL; U72289; AAB41187.1; -; Genomic_DNA.
DR RefSeq; NP_008082.1; NC_001566.1.
DR AlphaFoldDB; P34849; -.
DR SMR; P34849; -.
DR GeneID; 807701; -.
DR CTD; 4536; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
PE 3: Inferred from homology;
KW Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW NAD; Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW Transport; Ubiquinone.
FT CHAIN 1..333
FT /note="NADH-ubiquinone oxidoreductase chain 2"
FT /id="PRO_0000117550"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 57..77
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 91..111
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 170..190
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 192..212
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 242..262
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 267..287
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 313..333
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VARIANT 14
FT /note="F -> L (in strain: ssp. adansonii, ssp. capensis,
FT ssp. carnica, ssp. iberica, ssp. intermissa, ssp.
FT lamarckii, ssp. macedonica, ssp. meda, ssp. monticola, ssp.
FT sahariensis, ssp. scutellata and ssp. sicula)"
FT VARIANT 62
FT /note="V -> M (in haplotype 9)"
FT VARIANT 84
FT /note="T -> I (in haplotypes 3, 5, 6, 7 and 10)"
FT VARIANT 138
FT /note="V -> I (in haplotype 4, MELLI1 and MELLI2)"
FT VARIANT 142
FT /note="K -> S (in strain: ssp. lamarckii)"
FT VARIANT 158
FT /note="I -> T (in strain: MELLI1)"
FT VARIANT 203
FT /note="F -> L (in strain: ssp. carnica)"
FT VARIANT 262
FT /note="V -> D (in haplotype 6)"
SQ SEQUENCE 333 AA; 40879 MW; 866969F11529907F CRC64;
MFFMNFKYHW FIYFLITIFV LMMNSNNIFI QWMLMEFGTI ISISLINIKS TNKTPSLIYY
SVSVISSIFL FFMIIVYLSS ISFTKTDTFN FMVQMMFFLK IGTFPFHFWM IYSYEMMNWK
QIFLMSTLIK FIPIYMMVSM TKINSWTLYF LITNSLYISF YANKFYTLKK LLACSTIFNS
FYFIFILELN KNMFIAMIIL YSFNYFLLIS FLNKFNIQNF NFMFYNKYQM YTFLTLMFNY
SMYPIFLSFV IKWNLIFMMV SVKAYNWILF LLMISSMLMI WNYIIILKRV FLKMNFYKNN
FIDDKDNKYM YHSYFALTLL SFNISFFITL NFL
