ID   NU2M_BETVU              Reviewed;         515 AA.
AC   P15688;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 2.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 2;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 2;
GN   Name=ND2; Synonyms=NAD2;
OS   Beta vulgaris (Sugar beet).
OG   Mitochondrion.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   Caryophyllales; Chenopodiaceae; Betoideae; Beta.
OX   NCBI_TaxID=161934;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2115110; DOI=10.1007/bf00261720;
RA   Xue Y., Davies D.R., Thomas C.M.;
RT   "Sugarbeet mitochondria contain an open reading frame showing extensive
RT   sequence homology to the subunit 2 gene of the NADH: ubiquinone reductase
RT   complex.";
RL   Mol. Gen. Genet. 221:195-198(1990).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane; Multi-pass membrane
CC       protein.
CC   -!- SIMILARITY: Belongs to the complex I subunit 2 family. {ECO:0000305}.
CC   -!- CAUTION: It is uncertain whether Met-1 or Met-52 is the initiator.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA34729.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; X16828; CAA34728.1; -; Genomic_DNA.
DR   EMBL; X16828; CAA34729.1; ALT_INIT; Genomic_DNA.
DR   PIR; S12804; DNETU2.
DR   AlphaFoldDB; P15688; -.
DR   SMR; P15688; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009536; C:plastid; IEA:UniProt.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   Pfam; PF00361; Proton_antipo_M; 1.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   NAD; Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW   Transport; Ubiquinone.
FT   CHAIN           1..515
FT                   /note="NADH-ubiquinone oxidoreductase chain 2"
FT                   /id="PRO_0000117558"
FT   TRANSMEM        63..83
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        250..270
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        299..319
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        356..376
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        379..399
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   515 AA;  56275 MW;  3D60FA5FBC2820AF CRC64;
     MLSLTIKGKA RRRKERAFGD RDFLTFSSKT KKTENVNLSF EKGTRFFDRG GMIFGPSPRS
     ARWPIGIAAF GLCLLFLIKN SGSARESAGN NRKEGVHVAA ASAPFLVNRA AGSATTTKER
     IHFKITNASA MAACGMAGSD LFGYIIQVES GVTGTAGLME NNFHGSVQRA LFSLRILRSL
     RVNSLARIQN FWGPSIPSSS PAKTPLPFGL NIFFDSYMWA PDIYEGSPTP VTAFFSIAPE
     RSISANILRV FIYGSYGATL QQIFFFCSIA LRLRSTGAMA NEGKASSSIG QLDYGGLYFV
     LVLMWNREGI QSLLIGLFIY ASMDDRCFAI VSALRQTRVK YIADLGALAK TNPISAITFS
     ITMFSYAGIP PLAGFCSKFY LFFAALGCGA YFLAPVGVVT SVIGCWAAGR LPRVSQFGDR
     RQFSVHRTRS LPNQLRHGWE CMLRKIGSSL IHQPSVYSIS LYESTITTRD EPWFGEFELA
     LGVIGLPVTA HDRILRCSPP VVGTTRAGPG LNSER