NU2M_CAEBR
ID NU2M_CAEBR Reviewed; 282 AA.
AC Q8HEC1; B1PE37; B1PE49; B1PE61; B1PEC1; B1PED3; B1PEE5; B1PEF7; B1PEI1;
AC B1PEK5; B1PEQ3; B1PET9;
DT 19-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 13-OCT-2009, sequence version 3.
DT 25-MAY-2022, entry version 64.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 2;
DE EC=7.1.1.2;
DE AltName: Full=NADH dehydrogenase subunit 2;
GN Name=nd2;
OS Caenorhabditis briggsae.
OG Mitochondrion {ECO:0000312|EMBL:AAO13512.1}.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6238 {ECO:0000312|EMBL:AAO13512.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS PHE-85; SER-194; PHE-262
RP AND ASN-265.
RC STRAIN=PB800;
RX PubMed=12644560; DOI=10.1093/molbev/msg044;
RA Denver D.R., Morris K., Thomas W.K.;
RT "Phylogenetics in Caenorhabditis elegans: an analysis of divergence and
RT outcrossing.";
RL Mol. Biol. Evol. 20:393-400(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS MET-24; SER-25; VAL-76;
RP PHE-85; SER-91; ALA-125; PHE-128; THR-137; VAL-164; LEU-190; SER-194;
RP ILE-202; MET-203; VAL-203; THR-209; SER-259; PHE-262; SER-264 AND ASN-265.
RC STRAIN=BW287, ED3032, ED3033, ED3034, ED3035, ED3036, ED3037, ED3083,
RC ED3092, ED3101, EG4181, EG4207A, HK104, HK105, JU403, JU439, JU516, JU725,
RC JU726, JU793, PB800, PB826, and VT847;
RX PubMed=18302772; DOI=10.1186/1471-2148-8-62;
RA Howe D.K., Denver D.R.;
RT "Muller's Ratchet and compensatory mutation in Caenorhabditis briggsae
RT mitochondrial genome evolution.";
RL BMC Evol. Biol. 8:62-62(2008).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AF16;
RX PubMed=14624247; DOI=10.1371/journal.pbio.0000045;
RA Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N.,
RA Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P.,
RA Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W.,
RA Hillier L.W., Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A.,
RA Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E.,
RA Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K.,
RA Durbin R.M., Waterston R.H.;
RT "The genome sequence of Caenorhabditis briggsae: a platform for comparative
RT genomics.";
RL PLoS Biol. 1:166-192(2003).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane; Multi-pass membrane
CC protein.
CC -!- SIMILARITY: Belongs to the complex I subunit 2 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAO13512.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AY171105; AAO13512.1; ALT_INIT; Genomic_DNA.
DR EMBL; EU407781; ACB06104.1; -; Genomic_DNA.
DR EMBL; EU407782; ACB06116.1; -; Genomic_DNA.
DR EMBL; EU407783; ACB06128.1; -; Genomic_DNA.
DR EMBL; EU407784; ACB06140.1; -; Genomic_DNA.
DR EMBL; EU407785; ACB06152.1; -; Genomic_DNA.
DR EMBL; EU407786; ACB06164.1; -; Genomic_DNA.
DR EMBL; EU407787; ACB06176.1; -; Genomic_DNA.
DR EMBL; EU407788; ACB06188.1; -; Genomic_DNA.
DR EMBL; EU407789; ACB06200.1; -; Genomic_DNA.
DR EMBL; EU407790; ACB06212.1; -; Genomic_DNA.
DR EMBL; EU407791; ACB06224.1; -; Genomic_DNA.
DR EMBL; EU407792; ACB06236.1; -; Genomic_DNA.
DR EMBL; EU407793; ACB06248.1; -; Genomic_DNA.
DR EMBL; EU407794; ACB06260.1; -; Genomic_DNA.
DR EMBL; EU407795; ACB06272.1; -; Genomic_DNA.
DR EMBL; EU407796; ACB06284.1; -; Genomic_DNA.
DR EMBL; EU407797; ACB06296.1; -; Genomic_DNA.
DR EMBL; EU407798; ACB06308.1; -; Genomic_DNA.
DR EMBL; EU407799; ACB06320.1; -; Genomic_DNA.
DR EMBL; EU407800; ACB06332.1; -; Genomic_DNA.
DR EMBL; EU407801; ACB06344.1; -; Genomic_DNA.
DR EMBL; EU407802; ACB06356.1; -; Genomic_DNA.
DR EMBL; EU407803; ACB06368.1; -; Genomic_DNA.
DR EMBL; AC186293; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; YP_001504335.1; NC_009885.1.
DR AlphaFoldDB; Q8HEC1; -.
DR SMR; Q8HEC1; -.
DR GeneID; 5666633; -.
DR KEGG; cbr:ND2; -.
DR CTD; 4536; -.
DR InParanoid; Q8HEC1; -.
DR Proteomes; UP000008549; Mitochondrion.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
PE 3: Inferred from homology;
KW Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW NAD; Reference proteome; Respiratory chain; Translocase; Transmembrane;
KW Transmembrane helix; Transport; Ubiquinone.
FT CHAIN 1..282
FT /note="NADH-ubiquinone oxidoreductase chain 2"
FT /id="PRO_0000117563"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 58..78
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 115..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 166..186
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 202..222
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..252
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VARIANT 24
FT /note="V -> M (in strain: PB826)"
FT /evidence="ECO:0000269|PubMed:18302772"
FT VARIANT 25
FT /note="W -> S (in strain: ED3101)"
FT /evidence="ECO:0000269|PubMed:18302772"
FT VARIANT 76
FT /note="I -> V (in strain: ED3092 and ED3101)"
FT /evidence="ECO:0000269|PubMed:18302772"
FT VARIANT 85
FT /note="L -> F (in strain: BW287, EG4181, EG4207A, HK104,
FT HK105, JU403, JU439, JU516, JU793, PB800 and PB826)"
FT /evidence="ECO:0000269|PubMed:12644560,
FT ECO:0000269|PubMed:18302772"
FT VARIANT 91
FT /note="N -> S (in strain: ED3092 and ED3101)"
FT /evidence="ECO:0000269|PubMed:18302772"
FT VARIANT 125
FT /note="V -> A (in strain: HK104 and HK105)"
FT /evidence="ECO:0000269|PubMed:18302772"
FT VARIANT 128
FT /note="L -> F (in strain: ED3092 and ED3101)"
FT /evidence="ECO:0000269|PubMed:18302772"
FT VARIANT 137
FT /note="V -> T (in strain: ED3092 and ED3101)"
FT /evidence="ECO:0000269|PubMed:18302772"
FT VARIANT 164
FT /note="L -> V (in strain: ED3092 and ED3101)"
FT /evidence="ECO:0000269|PubMed:18302772"
FT VARIANT 190
FT /note="S -> L (in strain: ED3083)"
FT /evidence="ECO:0000269|PubMed:18302772"
FT VARIANT 194
FT /note="G -> S (in strain: BW287, EG4181, EG4207A, HK104,
FT HK105, JU403, JU439, JU516, JU793, PB800 and PB826)"
FT /evidence="ECO:0000269|PubMed:12644560,
FT ECO:0000269|PubMed:18302772"
FT VARIANT 202
FT /note="T -> I (in strain: JU403, JU439, JU516 and JU793)"
FT /evidence="ECO:0000269|PubMed:18302772"
FT VARIANT 203
FT /note="A -> M (in strain: ED3092 and ED3101)"
FT /evidence="ECO:0000269|PubMed:18302772"
FT VARIANT 203
FT /note="A -> V (in strain: BW287, JU403, JU439, JU516, JU793
FT and PB826)"
FT /evidence="ECO:0000269|PubMed:18302772"
FT VARIANT 209
FT /note="I -> T (in strain: JU726)"
FT /evidence="ECO:0000269|PubMed:18302772"
FT VARIANT 259
FT /note="N -> S (in strain: ED3092 and ED3101)"
FT /evidence="ECO:0000269|PubMed:18302772"
FT VARIANT 262
FT /note="L -> F (in strain: EG4181, EG4207A and PB800)"
FT /evidence="ECO:0000269|PubMed:12644560,
FT ECO:0000269|PubMed:18302772"
FT VARIANT 264
FT /note="G -> S (in strain: ED3092 and ED3101)"
FT /evidence="ECO:0000269|PubMed:18302772"
FT VARIANT 265
FT /note="S -> N (in strain: BW287, ED3033, ED3034, ED3035,
FT ED3037, ED3092, ED3101, EG4181, EG4207A, HK104, HK105,
FT JU403, JU439, JU516, JU725, JU793, PB800, PB826 and VT847)"
FT /evidence="ECO:0000269|PubMed:12644560,
FT ECO:0000269|PubMed:18302772"
SQ SEQUENCE 282 AA; 33164 MW; B9A099D42B987E39 CRC64;
MIVFISLFTI FLTVLSLLTN NIIVWWSIFL LMTVVFVLLN KSSKSYTSIF NYFVIQESLG
LLFLLFSSGL LQFFIILLKI GVAPLHFWIF NVTNNIFNYA LMWFLTFQKL PFLTILLQIF
WLSSVYILLM GLLICYVQIF VMKSYKNLLI ISSTESFNWI VLGLFFSMFN TLYLFVYYFL
LMILLISKFS KSSGYNFVNW ETALVFLNIP FSVSFFVKIF SLSEIFKFDS FFTLLLLFSM
FLSVLAFSFW LINLSMKNNE ELSGSNKMNY FIIFPLMVIS II
