NU2M_CHLRE
ID NU2M_CHLRE Reviewed; 382 AA.
AC P08740; P11659;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1988, sequence version 1.
DT 25-MAY-2022, entry version 104.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 2;
DE EC=7.1.1.2;
DE AltName: Full=NADH dehydrogenase subunit 2;
GN Name=ND2; Synonyms=NAD2;
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OG Mitochondrion.
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=CW15-2;
RX PubMed=3020517; DOI=10.1093/nar/14.18.7506;
RA Boer P.H., Gray M.W.;
RT "Nucleotide sequence of a protein coding region in Chlamydomonas
RT reinhardtii mitochondrial DNA.";
RL Nucleic Acids Res. 14:7506-7507(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cw15;
RA Gray M.W.;
RL Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 305-382.
RC STRAIN=cw15;
RX PubMed=3205746; DOI=10.1093/nar/16.23.11373;
RA Ma D.-P., Yang Y.-W., Hasnain S.;
RT "Nucleotide sequence of Chlamydomonas reinhardtii mitochondrial genes
RT coding for subunit 6 of NADH dehydrogenase and tRNATrp.";
RL Nucleic Acids Res. 16:11373-11373(1988).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 366-382.
RC STRAIN=cw15;
RX PubMed=2734113; DOI=10.1093/nar/17.10.3993;
RA Boer P.H., Gray M.W.;
RT "Nucleotide sequence of a region encoding subunit 6 of NADH dehydrogenase
RT (ND6) and tRNA(Trp) in Chlamydomonas reinhardtii mitochondrial DNA.";
RL Nucleic Acids Res. 17:3993-3993(1989).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane; Multi-pass membrane
CC protein.
CC -!- SIMILARITY: Belongs to the complex I subunit 2 family. {ECO:0000305}.
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DR EMBL; X54860; CAA38643.1; -; Genomic_DNA.
DR EMBL; U03843; AAB93444.1; -; Genomic_DNA.
DR EMBL; X66484; CAA47115.1; -; Genomic_DNA.
DR EMBL; X12939; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; S37608; S37608.
DR RefSeq; NP_042568.1; NC_001638.1.
DR AlphaFoldDB; P08740; -.
DR SMR; P08740; -.
DR STRING; 3055.AAB93444; -.
DR TCDB; 3.D.1.6.4; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR GeneID; 801491; -.
DR KEGG; cre:ChrepMp05; -.
DR eggNOG; KOG4668; Eukaryota.
DR HOGENOM; CLU_724351_0_0_1; -.
DR OrthoDB; 1153818at2759; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR InterPro; IPR001750; ND/Mrp_mem.
DR Pfam; PF00361; Proton_antipo_M; 2.
PE 3: Inferred from homology;
KW Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW NAD; Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW Transport; Ubiquinone.
FT CHAIN 1..382
FT /note="NADH-ubiquinone oxidoreductase chain 2"
FT /id="PRO_0000117570"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 33..53
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 54..74
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..108
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..143
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..178
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 188..208
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 212..232
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 239..259
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 260..280
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 284..304
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 305..325
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 366
FT /note="A -> R (in Ref. 3)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 382 AA; 42383 MW; 358658E8EDCFCAE9 CRC64;
MIELDLCFGL LLLILFGLLS LRNGHVSLAH IRFICQCWLV MITPLEVQDF ALCILTVVLL
QSFHSFEALL FLLLAYIGQL YMMHSCNLVS FYVCLEAQTL CVVVLCGLLA RGASTSFSVE
AALKFLLLSA MVSGMALFWF SAMYQRTGSL DMVGQETFWI LLVMLFKLGV APMHMWSVDL
YGSIPKSLLL YLSTAPKLSL FTFWASSWHH DFSVGVFILF SMFIGSIGAY GQPALRSLFA
YSTINEIGLL LLAVETAGFH TLYQHLGIYI ITQLLLWNLT DKRLFALCAV SLAGLPPFAG
FFGKAWIFWH AMSVQAFSLL AAALFCTLLS LVYYLRVIRL FWTAPVHTAA SFTGAPNQTT
LTSACAVALA FAPVMLVKPF VI
