NU2M_DROME
ID NU2M_DROME Reviewed; 341 AA.
AC P03896; B6E0P2; B6E0Q5; B6E0U4; Q34347; Q34348; Q9MGN7; Q9MGP1; Q9MJC9;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 13-JUL-2010, sequence version 3.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 2;
DE EC=7.1.1.2;
DE AltName: Full=NADH dehydrogenase subunit 2;
GN Name=mt:ND2; Synonyms=NADH2, ND2;
OS Drosophila melanogaster (Fruit fly).
OG Mitochondrion.
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS PHE-148 AND ILE-277.
RC STRAIN=Oregon-R, and Zimbabwe 53;
RX PubMed=10903372; DOI=10.1007/s002390010066;
RA Ballard J.W.O.;
RT "Comparative genomics of mitochondrial DNA in members of the Drosophila
RT melanogaster subgroup.";
RL J. Mol. Evol. 51:48-63(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Paris;
RX PubMed=11298822; DOI=10.1046/j.1365-2540.2001.00814.x;
RA Azou Y., Bregliano J.C.;
RT "I-R system of hybrid dysgenesis in Drosophila melanogaster: analysis of
RT the mitochondrial DNA in reactive strains exhibiting different potentials
RT for I factor transposition.";
RL Heredity 86:110-116(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS TYR-182 AND ILE-277.
RC STRAIN=Alstonvl, Brownsvl, Dahomey, Japan, Mysore, and W1118iso;
RX PubMed=18727704; DOI=10.1111/j.1474-9726.2008.00428.x;
RA Clancy D.J.;
RT "Variation in mitochondrial genotype has substantial lifespan effects which
RT may be modulated by nuclear background.";
RL Aging Cell 7:795-804(2008).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT ILE-277.
RC STRAIN=Oregon-R;
RX PubMed=19540318; DOI=10.1016/j.gene.2009.06.006;
RA Stewart J.B., Beckenbach A.T.;
RT "Characterization of mature mitochondrial transcripts in Drosophila, and
RT the implications for the tRNA punctuation model in arthropods.";
RL Gene 445:49-57(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANTS LEU-200 AND
RP ILE-277.
RX PubMed=8825764; DOI=10.1111/j.1365-2583.1995.tb00032.x;
RA Lewis D.L., Farr C.L., Kaguni L.S.;
RT "Drosophila melanogaster mitochondrial DNA: completion of the nucleotide
RT sequence and evolutionary comparisons.";
RL Insect Mol. Biol. 4:263-278(1995).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RA Wan K., Celniker S.;
RL Submitted (AUG-2014) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 56-341, AND VARIANTS LEU-200 AND
RP ILE-277.
RC STRAIN=Oregon-R; TISSUE=Embryo;
RX PubMed=6408489; DOI=10.1038/304234a0;
RA de Bruijn M.H.L.;
RT "Drosophila melanogaster mitochondrial DNA, a novel organization and
RT genetic code.";
RL Nature 304:234-241(1983).
RN [8]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 68-341, AND VARIANT ILE-277.
RC STRAIN=SP1;
RX PubMed=2124697; DOI=10.1073/pnas.87.24.9558;
RA Satta Y., Takahata N.;
RT "Evolution of Drosophila mitochondrial DNA and the history of the
RT melanogaster subgroup.";
RL Proc. Natl. Acad. Sci. U.S.A. 87:9558-9562(1990).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane; Multi-pass membrane
CC protein.
CC -!- SIMILARITY: Belongs to the complex I subunit 2 family. {ECO:0000305}.
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DR EMBL; AF200828; AAF77226.1; -; Genomic_DNA.
DR EMBL; AF200829; AAF77239.1; -; Genomic_DNA.
DR EMBL; AJ400907; CAB91051.1; -; Genomic_DNA.
DR EMBL; FJ190105; ACI28542.1; -; Genomic_DNA.
DR EMBL; FJ190106; ACI28555.1; -; Genomic_DNA.
DR EMBL; FJ190107; ACI28568.1; -; Genomic_DNA.
DR EMBL; FJ190108; ACI28581.1; -; Genomic_DNA.
DR EMBL; FJ190109; ACI28594.1; -; Genomic_DNA.
DR EMBL; FJ190110; ACI28607.1; -; Genomic_DNA.
DR EMBL; GQ229518; ACT21543.1; -; mRNA.
DR EMBL; U37541; AAC47811.1; -; Genomic_DNA.
DR EMBL; KJ947872; AIC64004.1; -; Genomic_DNA.
DR EMBL; J01404; AAB59238.1; -; Genomic_DNA.
DR EMBL; M57910; AAB02281.1; -; Genomic_DNA.
DR PIR; A00419; QXFF2M.
DR RefSeq; YP_009047266.1; NC_024511.2.
DR AlphaFoldDB; P03896; -.
DR SMR; P03896; -.
DR STRING; 7227.FBpp0100175; -.
DR PaxDb; P03896; -.
DR EnsemblMetazoa; FBtr0100857; FBpp0100175; FBgn0013680.
DR GeneID; 19893529; -.
DR KEGG; dme:Dmel_CG34063; -.
DR CTD; 4536; -.
DR FlyBase; FBgn0013680; mt:ND2.
DR VEuPathDB; VectorBase:FBgn0013680; -.
DR eggNOG; KOG4668; Eukaryota.
DR GeneTree; ENSGT00730000111348; -.
DR HOGENOM; CLU_007100_1_3_1; -.
DR InParanoid; P03896; -.
DR OrthoDB; 1153818at2759; -.
DR PhylomeDB; P03896; -.
DR Reactome; R-DME-611105; Respiratory electron transport.
DR Reactome; R-DME-6799198; Complex I biogenesis.
DR GenomeRNAi; 19893529; -.
DR PRO; PR:P03896; -.
DR Proteomes; UP000000803; Mitochondrion.
DR Bgee; FBgn0013680; Expressed in midgut and 15 other tissues.
DR ExpressionAtlas; P03896; baseline and differential.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; IMP:FlyBase.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IBA:GO_Central.
DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; IBA:GO_Central.
DR InterPro; IPR010933; NADH_DH_su2_C.
DR InterPro; IPR003917; NADH_UbQ_OxRdtase_chain2.
DR InterPro; IPR001750; ND/Mrp_mem.
DR Pfam; PF06444; NADH_dehy_S2_C; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR PRINTS; PR01436; NADHDHGNASE2.
PE 2: Evidence at transcript level;
KW Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW NAD; Reference proteome; Respiratory chain; Translocase; Transmembrane;
KW Transmembrane helix; Transport; Ubiquinone.
FT CHAIN 1..341
FT /note="NADH-ubiquinone oxidoreductase chain 2"
FT /id="PRO_0000117581"
FT TRANSMEM 8..28
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 146..166
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 195..215
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 238..258
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 273..293
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 321..341
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VARIANT 148
FT /note="Y -> F (in strain: Zimbabwe 53)"
FT /evidence="ECO:0000269|PubMed:10903372"
FT VARIANT 182
FT /note="H -> Y (in strain: Japan)"
FT /evidence="ECO:0000269|PubMed:18727704"
FT VARIANT 200
FT /note="Y -> L (in strain: Oregon-R)"
FT /evidence="ECO:0000269|PubMed:6408489,
FT ECO:0000269|PubMed:8825764"
FT VARIANT 277
FT /note="L -> I (in strain: Oregon-R, SP1 and w1118iso)"
FT /evidence="ECO:0000269|PubMed:10903372,
FT ECO:0000269|PubMed:18727704, ECO:0000269|PubMed:19540318,
FT ECO:0000269|PubMed:2124697, ECO:0000269|PubMed:6408489,
FT ECO:0000269|PubMed:8825764"
SQ SEQUENCE 341 AA; 39823 MW; 2D49E534B44B0778 CRC64;
MFNNSSKILF ITIMIIGTLI TVTSNSWLGA WMGLEINLLS FIPLLSDNNN LMSTEASLKY
FLTQVLASTV LLFSSILLML KNNMNNEINE SFTSMIIMSA LLLKSGAAPF HFWFPNMMEG
LTWMNALMLM TWQKIAPLML ISYLNIKYLL LISVILSVII GAIGGLNQTS LRKLMAFSSI
NHLGWMLSSL MISESIWLIY FFFYSFLSFV LTFMFNIFKL FHLNQLFSWF VNSKILKFTL
FMNFLSLGGL PPFLGFLPKW LVIQQLTLCN QYFMLTLMMM STLITLFFYL RICYSAFMMN
YFENNWIMKM NMNSINYNMY MIMTFFSIFG LFLISLFYFM F
