ID   NU2M_FORLA              Reviewed;         348 AA.
AC   P34187;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 2;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 2;
GN   Name=MT-ND2; Synonyms=MTND2, NADH2, ND2;
OS   Formosania lacustris (Oriental stream loach) (Crossostoma lacustre).
OG   Mitochondrion.
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Gastromyzontidae; Formosania.
OX   NCBI_TaxID=7980;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1408800; DOI=10.1093/nar/20.18.4853;
RA   Tzeng C.-S., Hui C.-F., Shen S.-C., Huang P.C.;
RT   "The complete nucleotide sequence of the Crossostoma lacustre mitochondrial
RT   genome: conservation and variations among vertebrates.";
RL   Nucleic Acids Res. 20:4853-4858(1992).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane; Multi-pass membrane
CC       protein.
CC   -!- SIMILARITY: Belongs to the complex I subunit 2 family. {ECO:0000305}.
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DR   EMBL; M91245; AAB96812.1; -; Genomic_DNA.
DR   PIR; S35463; S35463.
DR   RefSeq; NP_008304.1; NC_001727.1.
DR   AlphaFoldDB; P34187; -.
DR   SMR; P34187; -.
DR   GeneID; 807991; -.
DR   CTD; 4536; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; IEA:InterPro.
DR   InterPro; IPR010933; NADH_DH_su2_C.
DR   InterPro; IPR003917; NADH_UbQ_OxRdtase_chain2.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   Pfam; PF06444; NADH_dehy_S2_C; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   PRINTS; PR01436; NADHDHGNASE2.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   NAD; Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW   Transport; Ubiquinone.
FT   CHAIN           1..348
FT                   /note="NADH-ubiquinone oxidoreductase chain 2"
FT                   /id="PRO_0000117573"
FT   TRANSMEM        3..23
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        25..45
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        60..80
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        99..119
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        122..142
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        150..170
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        178..196
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        200..219
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        246..266
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        274..294
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        328..348
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   348 AA;  37925 MW;  146EF060DC6FF2F7 CRC64;
     MNPYVLMILI SSLGLGTTLT FASSHWLLAW MGLEINTLAI LRLMAQQHHP RAVEATTKYF
     LTQATAAAMI LFAATTNAWA TGEWDINNLT HPLASSLTMM ALALKVGLAP MHFWMPEVLQ
     GLDLTMGLIL STWQKLAPFA LITQMAPNTN PMLLTTLGLL STLIGGWGGL NQTQLRKILA
     YSSIAHMGWM IIILQYTPQL TLIALGLYIF MTSAAFLSLK MASATKMNTL TAAWSKSPVL
     VSTTALTLLS LGGLPPLTGF MPKWLILQEL TKQDLPATAT IMALAALLSL YFYLRLSYAM
     ALTIYPNTTN ALTPWRTNTT QSTLALALMM IGALGLLPLT PTIMALFF