ID   NU2M_HORSE              Reviewed;         346 AA.
AC   P48653;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 2 {ECO:0000250|UniProtKB:P03891};
DE            EC=7.1.1.2 {ECO:0000250|UniProtKB:P03891};
DE   AltName: Full=NADH dehydrogenase subunit 2;
GN   Name=MT-ND2 {ECO:0000250|UniProtKB:P03891}; Synonyms=MTND2, NADH2, ND2;
OS   Equus caballus (Horse).
OG   Mitochondrion.
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX   NCBI_TaxID=9796;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Thoroughbred {ECO:0000312|Proteomes:UP000002281};
RX   PubMed=7958969; DOI=10.1016/0378-1119(94)90713-7;
RA   Xu X., Arnason U.;
RT   "The complete mitochondrial DNA sequence of the horse, Equus caballus:
RT   extensive heteroplasmy of the control region.";
RL   Gene 148:357-362(1994).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) which catalyzes electron transfer from
CC       NADH through the respiratory chain, using ubiquinone as an electron
CC       acceptor. Essential for the catalytic activity and assembly of complex
CC       I. {ECO:0000250|UniProtKB:P03891}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC         Evidence={ECO:0000250|UniProtKB:P03891};
CC   -!- SUBUNIT: Core subunit of respiratory chain NADH dehydrogenase (Complex
CC       I) which is composed of 45 different subunits. Interacts with TMEM242
CC       (By similarity). {ECO:0000250|UniProtKB:P03891,
CC       ECO:0000250|UniProtKB:P03892}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:P03892}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 2 family. {ECO:0000305}.
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DR   EMBL; X79547; CAA56080.1; -; Genomic_DNA.
DR   PIR; T11858; T11858.
DR   RefSeq; NP_007161.1; NC_001640.1.
DR   AlphaFoldDB; P48653; -.
DR   SMR; P48653; -.
DR   STRING; 9796.ENSECAP00000023101; -.
DR   PaxDb; P48653; -.
DR   Ensembl; ENSECAT00000029834; ENSECAP00000023101; ENSECAG00000027681.
DR   GeneID; 807844; -.
DR   KEGG; ecb:807844; -.
DR   CTD; 4536; -.
DR   VGNC; VGNC:59017; MT-ND2.
DR   GeneTree; ENSGT00730000111348; -.
DR   HOGENOM; CLU_007100_1_3_1; -.
DR   InParanoid; P48653; -.
DR   OMA; HFWVPEV; -.
DR   OrthoDB; 1153818at2759; -.
DR   Proteomes; UP000002281; Mitochondrion.
DR   Bgee; ENSECAG00000027681; Expressed in gluteus medius and 23 other tissues.
DR   ExpressionAtlas; P48653; baseline.
DR   GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR   GO; GO:0005747; C:mitochondrial respiratory chain complex I; IBA:GO_Central.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; ISS:UniProtKB.
DR   GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; ISS:UniProtKB.
DR   GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR   GO; GO:0072593; P:reactive oxygen species metabolic process; IEA:Ensembl.
DR   InterPro; IPR010933; NADH_DH_su2_C.
DR   InterPro; IPR003917; NADH_UbQ_OxRdtase_chain2.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   Pfam; PF06444; NADH_dehy_S2_C; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   PRINTS; PR01436; NADHDHGNASE2.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   NAD; Reference proteome; Respiratory chain; Translocase; Transmembrane;
KW   Transmembrane helix; Transport; Ubiquinone.
FT   CHAIN           1..346
FT                   /note="NADH-ubiquinone oxidoreductase chain 2"
FT                   /id="PRO_0000117594"
FT   TRANSMEM        3..23
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        25..45
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        59..79
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        96..116
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        122..142
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        149..169
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        178..198
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        200..220
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        242..262
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        274..294
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        322..342
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   346 AA;  39061 MW;  57DBC8E39D980A35 CRC64;
     MNPLIFTTIL MTVLLGTMIV MMSSHWLMIW IGFEMNLLAI IPILMKKYNP RTMEASTKYF
     LTQATASMLL MMAIIINLMH SGQWTITKVF NPTASIIMTS ALAMKLGLTP FHFWVPEVTQ
     GISLTSGLIL LTWQKLAPMS ILYQISPSIN LNILLTMAVL SILVGGWGGL NQTQLRKIMA
     YSSIAHMGWM TAVLVYNPTL TMLNMLIYIM MTLTMFMLFI HSSSTTTLSL SHTWNKMPLT
     TTLILITLLS MGGLPPLSGF MPKWMIIQEL TKNSSIILPT LMAIMALLNL YFYMRLTYST
     SLTMFPSTNN MKMKWQFETK RITLLPPLIV MSSLLLPLTP MLSILD