ID   NU2M_HYACU              Reviewed;         428 AA.
AC   Q950U6;
DT   19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 2 {ECO:0000250|UniProtKB:Q9B6C8};
DE            EC=7.1.1.2 {ECO:0000312|EMBL:AAK83420.1};
DE   AltName: Full=NADH dehydrogenase subunit 2 {ECO:0000312|EMBL:AAK83420.1};
GN   Name=NAD2 {ECO:0000312|EMBL:AAK83420.1};
OS   Hyaloraphidium curvatum (Lower fungus).
OG   Mitochondrion {ECO:0000312|EMBL:AAK83420.1}.
OC   Eukaryota; Fungi; Fungi incertae sedis; Chytridiomycota;
OC   Chytridiomycota incertae sedis; Monoblepharidomycetes; Monoblepharidales;
OC   Monoblepharidales incertae sedis; Hyaloraphidium.
OX   NCBI_TaxID=82268;
RN   [1] {ECO:0000312|EMBL:AAK83420.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SAG 235-1 / CCAP 235/1 {ECO:0000269|PubMed:11861890};
RX   PubMed=11861890; DOI=10.1093/oxfordjournals.molbev.a004084;
RA   Forget L., Ustinova J., Wang Z., Huss V.A.R., Lang B.F.;
RT   "Hyaloraphidium curvatum: a linear mitochondrial genome, tRNA editing, and
RT   an evolutionary link to lower fungi.";
RL   Mol. Biol. Evol. 19:310-319(2002).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250, ECO:0000305}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:P03892}; Multi-pass membrane protein
CC       {ECO:0000250|UniProtKB:P03892}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 2 family. {ECO:0000255}.
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DR   EMBL; AF402142; AAK83420.1; -; Genomic_DNA.
DR   RefSeq; NP_150098.1; NC_003048.1.
DR   AlphaFoldDB; Q950U6; -.
DR   SMR; Q950U6; -.
DR   GeneID; 803643; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   Pfam; PF00361; Proton_antipo_M; 1.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   NAD; Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW   Transport; Ubiquinone.
FT   CHAIN           1..428
FT                   /note="NADH-ubiquinone oxidoreductase chain 2"
FT                   /id="PRO_0000413002"
FT   TRANSMEM        22..42
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        59..79
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        84..104
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        108..128
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        140..160
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        185..205
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        218..238
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        241..261
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        269..289
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        292..312
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        332..352
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        356..376
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        384..404
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        408..428
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   428 AA;  47550 MW;  E1D428A2839C6EC7 CRC64;
     MLYLLLTTTL LMMTWNRYAG RIAFLSLLGY FVIMLNIWLH FGSLSESVTL LFGGGLVRLD
     ESILSALLFI LFLGLVIMNR TGDLGWEFHL LLMGGLTGAI YMLTAYDLLL MVVGFEFLNL
     STYLILSLYR GTETATLKYL LSSAFYTTLL LLAISFFYGL TGSTGYDALF VQMNYLGGET
     FLPQILLLGT IAFKLGLVPA HLWVPDVYDG LPMTLLSWMG SVPKAAVLLW LPTIYPLLNQ
     LAPFLLVLSA LSFLLSAVLM AAQYKMKRFL AYSAIGHLGF VVAAFAIGDY HAYGYYIFIY
     MIATLAQFVL LSELPQTELM KQTSVLKDHR ALGLGFLVIV LTMASLPPFA GFYAKLLVLF
     GFLEIGYGIF AVLLILASLR SAAYYLKWIQ TTFFSVVPFA SAPIQVQYPN LVSFLVTLIL
     PSTLLLLL