NU2M_LITCT
ID NU2M_LITCT Reviewed; 344 AA.
AC P16673;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 1.
DT 25-MAY-2022, entry version 92.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 2;
DE EC=7.1.1.2;
DE AltName: Full=NADH dehydrogenase subunit 2;
GN Name=MT-ND2; Synonyms=MTND2, NADH2, ND2;
OS Lithobates catesbeianus (American bullfrog) (Rana catesbeiana).
OG Mitochondrion.
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Neobatrachia; Ranoidea; Ranidae; Lithobates.
OX NCBI_TaxID=8400;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3497168; DOI=10.1272/jnms1923.54.277;
RA Fujii H.;
RT "Cloning of the entire mitochondrial genome of Rana catesbeiana and
RT nucleotide sequencing of the URF2 and its flanking genes.";
RL Nippon Ika Daigaku Zasshi 54:277-289(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3260590; DOI=10.1093/oxfordjournals.jbchem.a122295;
RA Fujii H., Shimada T., Goto Y., Okazaki T.;
RT "Cloning of the mitochondrial genome of Rana catesbeiana and the nucleotide
RT sequences of the ND2 and five tRNA genes.";
RL J. Biochem. 103:474-481(1988).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 325-344.
RX PubMed=2444617; DOI=10.1272/jnms1923.54.429;
RA Yoneyama Y.;
RT "The nucleotide sequences of the heavy and light strand replication origins
RT of the Rana catesbeiana mitochondrial genome.";
RL Nippon Ika Daigaku Zasshi 54:429-440(1987).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane; Multi-pass membrane
CC protein.
CC -!- SIMILARITY: Belongs to the complex I subunit 2 family. {ECO:0000305}.
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DR EMBL; D10368; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; D12695; BAA02192.1; -; Genomic_DNA.
DR PIR; A41451; QXFG2B.
DR AlphaFoldDB; P16673; -.
DR SMR; P16673; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; IEA:InterPro.
DR InterPro; IPR010933; NADH_DH_su2_C.
DR InterPro; IPR003917; NADH_UbQ_OxRdtase_chain2.
DR InterPro; IPR001750; ND/Mrp_mem.
DR Pfam; PF06444; NADH_dehy_S2_C; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR PRINTS; PR01436; NADHDHGNASE2.
PE 3: Inferred from homology;
KW Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW NAD; Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW Transport; Ubiquinone.
FT CHAIN 1..344
FT /note="NADH-ubiquinone oxidoreductase chain 2"
FT /id="PRO_0000117632"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 24..44
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 94..114
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 150..170
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 201..221
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 245..265
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 273..293
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 324..344
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 344 AA; 37625 MW; 32CB282EAC84153F CRC64;
MNPLALTIFL LSLAIGTTIT LSSFHWLLAW IGLEINTLAI IPLMTKTPHP RAIEAATKYF
LTQAAASALV LFSSLISAWQ TGEWSINSLM DLPMNILSIA LMMKLGLAPL HFWIPEVLQG
ISLPTGLILS TWQKIAPMAL LLQTSHLINL NLTIALGLTS IMVGGWGGIG QTQLRKIMAF
SSIGHLGWII VILKFDPQLS LLNFVLYIIM TAAMFMSLTT ISATKMLEIS TSWSKTPALT
TTTMLILLSL AGLPPLTGFT PKLLITLELV KQNATLLAVM VMFISLLALF FYIRLTYVVT
LTLSPNTPNS LLTWRTASRS YSTTAIMNTM ALILLPITPT LLLL
