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NU2M_MOUSE
ID   NU2M_MOUSE              Reviewed;         345 AA.
AC   P03893;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-2015, sequence version 2.
DT   03-AUG-2022, entry version 154.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 2 {ECO:0000305};
DE            EC=7.1.1.2 {ECO:0000250|UniProtKB:P03891};
DE   AltName: Full=NADH dehydrogenase subunit 2;
GN   Name=mt-Nd2 {ECO:0000312|MGI:MGI:102500}; Synonyms=Nd2;
OS   Mus musculus (Mouse).
OG   Mitochondrion.
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7332926; DOI=10.1016/0092-8674(81)90300-7;
RA   Bibb M.J., van Etten R.A., Wright C.T., Walberg M.W., Clayton D.A.;
RT   "Sequence and gene organization of mouse mitochondrial DNA.";
RL   Cell 26:167-180(1981).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=12954771; DOI=10.1093/nar/gkg739;
RA   Bayona-Bafaluy M.P., Acin-Perez R., Mullikin J.C., Park J.S.,
RA   Moreno-Loshuertos R., Hu P., Perez-Martos A., Fernandez-Silva P., Bai Y.,
RA   Enriquez J.A.;
RT   "Revisiting the mouse mitochondrial DNA sequence.";
RL   Nucleic Acids Res. 31:5349-5355(2003).
RN   [3]
RP   PROTEIN SEQUENCE OF 237-272 AND 296-312, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY.
RC   STRAIN=C57BL/6J, and OF1; TISSUE=Brain, and Hippocampus;
RA   Lubec G., Kang S.U., Sunyer B., Chen W.-Q.;
RL   Submitted (JAN-2009) to UniProtKB.
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brown adipose tissue, Heart, Kidney, and Liver;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) which catalyzes electron transfer from
CC       NADH through the respiratory chain, using ubiquinone as an electron
CC       acceptor. Essential for the catalytic activity and assembly of complex
CC       I. {ECO:0000250|UniProtKB:P03891}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC         Evidence={ECO:0000250|UniProtKB:P03891};
CC   -!- SUBUNIT: Core subunit of respiratory chain NADH dehydrogenase (Complex
CC       I) which is composed of 45 different subunits. Interacts with TMEM242
CC       (By similarity). {ECO:0000250|UniProtKB:P03891,
CC       ECO:0000250|UniProtKB:P03892}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:P03892}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 2 family. {ECO:0000305}.
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DR   EMBL; J01420; AAB48645.1; -; Genomic_DNA.
DR   EMBL; V00711; CAA24081.1; -; Genomic_DNA.
DR   EMBL; AY172335; AAN85123.1; -; Genomic_DNA.
DR   EMBL; AY339599; AAP89024.1; -; Genomic_DNA.
DR   PIR; A00416; QXMS2M.
DR   RefSeq; NP_904329.1; NC_005089.1.
DR   PDB; 6G2J; EM; 3.30 A; N=1-345.
DR   PDB; 6G72; EM; 3.90 A; N=1-345.
DR   PDB; 6ZR2; EM; 3.10 A; N=1-345.
DR   PDB; 6ZTQ; EM; 3.00 A; N=1-345.
DR   PDB; 7AK5; EM; 3.17 A; N=1-345.
DR   PDB; 7AK6; EM; 3.82 A; N=1-345.
DR   PDB; 7B93; EM; 3.04 A; N=1-345.
DR   PDB; 7PSA; EM; 3.40 A; N=1-345.
DR   PDBsum; 6G2J; -.
DR   PDBsum; 6G72; -.
DR   PDBsum; 6ZR2; -.
DR   PDBsum; 6ZTQ; -.
DR   PDBsum; 7AK5; -.
DR   PDBsum; 7AK6; -.
DR   PDBsum; 7B93; -.
DR   PDBsum; 7PSA; -.
DR   AlphaFoldDB; P03893; -.
DR   SMR; P03893; -.
DR   ComplexPortal; CPX-266; Mitochondrial respiratory chain complex I.
DR   IntAct; P03893; 3.
DR   MINT; P03893; -.
DR   STRING; 10090.ENSMUSP00000080992; -.
DR   PhosphoSitePlus; P03893; -.
DR   jPOST; P03893; -.
DR   PaxDb; P03893; -.
DR   PRIDE; P03893; -.
DR   ProteomicsDB; 287843; -.
DR   Antibodypedia; 47876; 63 antibodies from 19 providers.
DR   Ensembl; ENSMUST00000082396; ENSMUSP00000080992; ENSMUSG00000064345.
DR   GeneID; 17717; -.
DR   KEGG; mmu:17717; -.
DR   CTD; 4536; -.
DR   MGI; MGI:102500; mt-Nd2.
DR   VEuPathDB; HostDB:ENSMUSG00000064345; -.
DR   eggNOG; KOG4668; Eukaryota.
DR   GeneTree; ENSGT00730000111348; -.
DR   HOGENOM; CLU_007100_1_3_1; -.
DR   InParanoid; P03893; -.
DR   OMA; HFWVPEV; -.
DR   OrthoDB; 1153818at2759; -.
DR   PhylomeDB; P03893; -.
DR   Reactome; R-MMU-611105; Respiratory electron transport.
DR   Reactome; R-MMU-6799198; Complex I biogenesis.
DR   ChiTaRS; mt-Nd2; mouse.
DR   PRO; PR:P03893; -.
DR   Proteomes; UP000000589; Mitochondrion.
DR   RNAct; P03893; protein.
DR   Bgee; ENSMUSG00000064345; Expressed in dentate gyrus of hippocampal formation granule cell and 60 other tissues.
DR   ExpressionAtlas; P03893; baseline and differential.
DR   GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR   GO; GO:0005747; C:mitochondrial respiratory chain complex I; ISO:MGI.
DR   GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR   GO; GO:0014069; C:postsynaptic density; ISO:MGI.
DR   GO; GO:0035255; F:ionotropic glutamate receptor binding; ISO:MGI.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; ISS:UniProtKB.
DR   GO; GO:0019901; F:protein kinase binding; ISO:MGI.
DR   GO; GO:0009060; P:aerobic respiration; IC:ComplexPortal.
DR   GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; ISS:UniProtKB.
DR   GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR   GO; GO:0042776; P:proton motive force-driven mitochondrial ATP synthesis; IC:ComplexPortal.
DR   GO; GO:0072593; P:reactive oxygen species metabolic process; IDA:MGI.
DR   InterPro; IPR010933; NADH_DH_su2_C.
DR   InterPro; IPR003917; NADH_UbQ_OxRdtase_chain2.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   Pfam; PF06444; NADH_dehy_S2_C; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   PRINTS; PR01436; NADHDHGNASE2.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Electron transport; Membrane;
KW   Mitochondrion; Mitochondrion inner membrane; NAD; Reference proteome;
KW   Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW   Transport; Ubiquinone.
FT   CHAIN           1..345
FT                   /note="NADH-ubiquinone oxidoreductase chain 2"
FT                   /id="PRO_0000117607"
FT   TRANSMEM        1..21
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        25..45
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        56..76
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        92..114
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        149..171
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        178..198
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        200..220
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        241..261
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        274..294
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        324..344
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        294
FT                   /note="T -> I (in Ref. 1; AAB48645/CAA24081 and 2;
FT                   AAP89024)"
FT                   /evidence="ECO:0000305"
FT   HELIX           3..21
FT                   /evidence="ECO:0007829|PDB:6ZTQ"
FT   HELIX           26..44
FT                   /evidence="ECO:0007829|PDB:6ZTQ"
FT   HELIX           50..81
FT                   /evidence="ECO:0007829|PDB:6ZTQ"
FT   STRAND          82..85
FT                   /evidence="ECO:0007829|PDB:6ZTQ"
FT   STRAND          88..90
FT                   /evidence="ECO:0007829|PDB:7B93"
FT   HELIX           92..106
FT                   /evidence="ECO:0007829|PDB:6ZTQ"
FT   TURN            111..114
FT                   /evidence="ECO:0007829|PDB:6G2J"
FT   HELIX           115..120
FT                   /evidence="ECO:0007829|PDB:6ZTQ"
FT   TURN            125..127
FT                   /evidence="ECO:0007829|PDB:6ZTQ"
FT   HELIX           128..132
FT                   /evidence="ECO:0007829|PDB:6ZTQ"
FT   TURN            133..135
FT                   /evidence="ECO:0007829|PDB:6ZTQ"
FT   HELIX           136..144
FT                   /evidence="ECO:0007829|PDB:6ZTQ"
FT   HELIX           145..148
FT                   /evidence="ECO:0007829|PDB:6ZTQ"
FT   HELIX           153..170
FT                   /evidence="ECO:0007829|PDB:6ZTQ"
FT   HELIX           175..193
FT                   /evidence="ECO:0007829|PDB:6ZTQ"
FT   TURN            194..196
FT                   /evidence="ECO:0007829|PDB:7AK5"
FT   HELIX           199..222
FT                   /evidence="ECO:0007829|PDB:6ZTQ"
FT   HELIX           227..230
FT                   /evidence="ECO:0007829|PDB:6ZTQ"
FT   TURN            231..235
FT                   /evidence="ECO:0007829|PDB:6ZTQ"
FT   HELIX           238..240
FT                   /evidence="ECO:0007829|PDB:6ZTQ"
FT   HELIX           241..251
FT                   /evidence="ECO:0007829|PDB:6ZTQ"
FT   HELIX           258..260
FT                   /evidence="ECO:0007829|PDB:6ZTQ"
FT   HELIX           261..271
FT                   /evidence="ECO:0007829|PDB:6ZTQ"
FT   TURN            272..274
FT                   /evidence="ECO:0007829|PDB:6ZTQ"
FT   HELIX           276..301
FT                   /evidence="ECO:0007829|PDB:6ZTQ"
FT   STRAND          308..310
FT                   /evidence="ECO:0007829|PDB:7B93"
FT   HELIX           312..316
FT                   /evidence="ECO:0007829|PDB:6ZTQ"
FT   HELIX           325..332
FT                   /evidence="ECO:0007829|PDB:6ZTQ"
FT   STRAND          333..335
FT                   /evidence="ECO:0007829|PDB:6ZTQ"
FT   HELIX           337..342
FT                   /evidence="ECO:0007829|PDB:6ZTQ"
SQ   SEQUENCE   345 AA;  38752 MW;  F6855CA2CA3F2E59 CRC64;
     MNPITLAIIY FTIFLGPVIT MSSTNLMLMW VGLEFSLLAI IPMLINKKNP RSTEAATKYF
     VTQATASMII LLAIVLNYKQ LGTWMFQQQT NGLILNMTLM ALSMKLGLAP FHFWLPEVTQ
     GIPLHMGLIL LTWQKIAPLS ILIQIYPLLN STIILMLAIT SIFMGAWGGL NQTQMRKIMA
     YSSIAHMGWM LAILPYNPSL TLLNLMIYII LTAPMFMALM LNNSMTINSI SLLWNKTPAM
     LTMISLMLLS LGGLPPLTGF LPKWIIITEL MKNNCLIMAT LMAMMALLNL FFYTRLIYST
     SLTMFPTNNN SKMMTHQTKT KPNLMFSTLA IMSTMTLPLA PQLIT
 
 
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