ID   NU2M_PARTE              Reviewed;         193 AA.
AC   P15577;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 2;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 2;
GN   Name=ND2; Synonyms=NDH2;
OS   Paramecium tetraurelia.
OG   Mitochondrion.
OC   Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata;
OC   Oligohymenophorea; Peniculida; Parameciidae; Paramecium.
OX   NCBI_TaxID=5888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Stock 51;
RX   PubMed=2308823; DOI=10.1093/nar/18.1.173;
RA   Pritchard A.E., Seilhamer J.J., Mahalingam R., Sable C.L., Venuti S.E.,
RA   Cummings D.J.;
RT   "Nucleotide sequence of the mitochondrial genome of Paramecium.";
RL   Nucleic Acids Res. 18:173-180(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Stock 51;
RX   PubMed=2670676; DOI=10.1016/0378-1119(89)90320-x;
RA   Pritchard A.E., Venuti S.E., Ghalambor M.A., Sable C.L., Cummings D.J.;
RT   "An unusual region of Paramecium mitochondrial DNA containing chloroplast-
RT   like genes.";
RL   Gene 78:121-134(1989).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane; Multi-pass membrane
CC       protein.
CC   -!- CAUTION: No similarity to other species complex I subunit 2.
CC       {ECO:0000305}.
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DR   EMBL; M26930; AAA79255.1; -; Genomic_DNA.
DR   EMBL; X15917; CAA34043.1; -; Genomic_DNA.
DR   PIR; S07734; S07734.
DR   AlphaFoldDB; P15577; -.
DR   SMR; P15577; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   NAD; Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW   Transport; Ubiquinone.
FT   CHAIN           1..193
FT                   /note="NADH-ubiquinone oxidoreductase chain 2"
FT                   /id="PRO_0000117621"
FT   TRANSMEM        18..38
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        39..59
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        83..103
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        116..138
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        161..181
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   193 AA;  23181 MW;  E783FD28E238AE31 CRC64;
     MRAHLLHCEL AFSFGKYFYS TSFLNLLMIN LMFSKLGAIF FLNLALYLLA LALFFFFLFN
     VKVALLKSVS QIYYFNNIFF FKFFVLIFFL NLAGIPPLLG FFLKFLIFFF LFFKTNLAFI
     LIFLGFNMAT LFFYLSTVKS FVNRKQASVL NSFNFFIRAE LSFLYFFNFF YFFLFFAFFF
     LDSTFLIFLN LFF