ID   NU2M_PODAN              Reviewed;         556 AA.
AC   P15578;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 2;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 2;
GN   Name=ND2;
OS   Podospora anserina (strain S / ATCC MYA-4624 / DSM 980 / FGSC 10383)
OS   (Pleurage anserina).
OG   Mitochondrion.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Podosporaceae; Podospora;
OC   Podospora anserina.
OX   NCBI_TaxID=515849;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=A, and s;
RX   PubMed=2975708; DOI=10.1016/0022-2836(88)90044-7;
RA   Cummings D.J., Domenico J.M.;
RT   "Sequence analysis of mitochondrial DNA from Podospora anserina.
RT   Pervasiveness of a class I intron in three separate genes.";
RL   J. Mol. Biol. 204:815-839(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=s;
RX   PubMed=2357736; DOI=10.1007/bf00334517;
RA   Cummings D.J., McNally K.L., Domenico J.M., Matsuura E.T.;
RT   "The complete DNA sequence of the mitochondrial genome of Podospora
RT   anserina.";
RL   Curr. Genet. 17:375-402(1990).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane; Multi-pass membrane
CC       protein.
CC   -!- SIMILARITY: Belongs to the complex I subunit 2 family. {ECO:0000305}.
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DR   EMBL; X55026; CAA38765.1; -; Genomic_DNA.
DR   EMBL; X14485; CAA32646.1; -; Genomic_DNA.
DR   PIR; S02154; S02154.
DR   RefSeq; NP_074912.1; NC_001329.3.
DR   AlphaFoldDB; P15578; -.
DR   SMR; P15578; -.
DR   STRING; 515849.P15578; -.
DR   GeneID; 802484; -.
DR   KEGG; pan:PoanfMp04; -.
DR   Proteomes; UP000001197; Mitochondrion.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR   HAMAP; MF_00445; NDH1_NuoN_1; 1.
DR   InterPro; IPR010096; NADH-Q_OxRdtase_suN/2.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   Pfam; PF00361; Proton_antipo_M; 1.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   NAD; Reference proteome; Respiratory chain; Translocase; Transmembrane;
KW   Transmembrane helix; Transport; Ubiquinone.
FT   CHAIN           1..556
FT                   /note="NADH-ubiquinone oxidoreductase chain 2"
FT                   /id="PRO_0000117627"
FT   TRANSMEM        58..78
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        122..142
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        145..165
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        177..197
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        239..259
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        268..288
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        304..324
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        333..353
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        358..378
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        423..443
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        455..475
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        517..537
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   556 AA;  62554 MW;  5613ACB0D2324BAF CRC64;
     MIFISIIGLL LSNAVTLRQD MSVNFNRIAL IDLIYCILHD TMSLSIINKG IGLHGGLLHI
     TNITLIFHIF IFFLSILILQ LTSFYPRKAW IPEHSSLKDI IYQKFLNYRT KIFNKMGEHM
     KIIEYPLILL FVISGAVFLM STNDLVSIFL SIELQSYGLY LLSTIYRNSE LSTAGGLIYF
     LLGGLSSCFI LLGTSLLYIN SGTTSLDGLY ILNSISDVKD GAADMPALTS WYKSYYLNFA
     LLVFSIGFLF KVSAAPFHFW SPDVYDAIPT IVTTFVAIIA KISIFIFLLE LVYHTNNYLS
     EFSWTYLLLI SSLFSLIIGT VVGLTQFRIK RLLAYSTISH VGFILLALSG CSIESTQAFI
     FYLIQYSISN LNVFIIIITI GFSLYGYITT NKEYKDLLDK NNSPIQVISQ LKGYFYINPL
     LSLSLAITIF SFVGIPPLVG FFAKQMVLSA ALDNGYIFLT LIAILTSVIG AVYYLNIIKK
     IFFYLPDHSI NPSIGEFLFK KGLIFEAGDF KGRITLISSP FSITISIITL VILLFIFMNK
     EWLSMGTILV QVLFSN