ID   NU2M_SCYCA              Reviewed;         348 AA.
AC   O21409;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 2;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 2;
GN   Name=MT-ND2; Synonyms=MTND2, NADH2, ND2;
OS   Scyliorhinus canicula (Small-spotted catshark) (Squalus canicula).
OG   Mitochondrion.
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Chondrichthyes;
OC   Elasmobranchii; Galeomorphii; Galeoidea; Carcharhiniformes; Scyliorhinidae;
OC   Scyliorhinus.
OX   NCBI_TaxID=7830;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9254918; DOI=10.1093/oxfordjournals.molbev.a025821;
RA   Delarbre C., Barriel V., Tillier S., Janvier P., Gachelin G.;
RT   "The main features of the craniate mitochondrial DNA between the ND1 and
RT   the COI genes were established in the common ancestor with the lancelet.";
RL   Mol. Biol. Evol. 14:807-813(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Muscle;
RX   PubMed=9725850; DOI=10.1093/genetics/150.1.331;
RA   Delarbre C., Spruyt N., Delmarre C., Gallut C., Barriel V., Janvier P.,
RA   Laudet V., Gachelin G.;
RT   "The complete nucleotide sequence of the mitochondrial DNA of the dogfish,
RT   Scyliorhinus canicula.";
RL   Genetics 150:331-344(1998).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane; Multi-pass membrane
CC       protein.
CC   -!- SIMILARITY: Belongs to the complex I subunit 2 family. {ECO:0000305}.
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DR   EMBL; Y09526; CAA70714.1; -; Genomic_DNA.
DR   EMBL; Y16067; CAA76020.1; -; Genomic_DNA.
DR   PIR; T11301; T11301.
DR   RefSeq; NP_007615.1; NC_001950.1.
DR   AlphaFoldDB; O21409; -.
DR   SMR; O21409; -.
DR   GeneID; 808291; -.
DR   CTD; 4536; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; IEA:InterPro.
DR   InterPro; IPR010933; NADH_DH_su2_C.
DR   InterPro; IPR003917; NADH_UbQ_OxRdtase_chain2.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   Pfam; PF06444; NADH_dehy_S2_C; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   PRINTS; PR01436; NADHDHGNASE2.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   NAD; Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW   Transport; Ubiquinone.
FT   CHAIN           1..348
FT                   /note="NADH-ubiquinone oxidoreductase chain 2"
FT                   /id="PRO_0000117637"
FT   TRANSMEM        3..23
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        25..45
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        59..79
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        95..115
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        149..171
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        178..198
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        199..219
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        242..262
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        274..294
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        324..344
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   348 AA;  38136 MW;  928AFBAEE45400EF CRC64;
     MNPTVLTIII SSMGLGTTLT FIGSHWLLVW MGLEINTLAI IPLMIRQHHP RAVEATTKYF
     ITQATASALL LFASVTNAWT SGEWSLIEML NPTSATLATA ALALKIGLAP LHFWLPEVLQ
     GLDLTTGLIL ATWQKLAPFA ILLQLSPLLN SNLLLLFGVT STIVGGWGGL NQTQLRKILA
     YSSIANLGWM ITILHYSPSL TLLNLILYMF MTLTTFLLFK TFNSTKINSI SSSTLKSPLM
     SVIALMTLLS LGGLPPLSGF MPKWLILQEL TKQSLIIPAT IMALMALLSL FFYLRLCYAT
     TLTKAPGPIN MASTWRTKSH QPTLILLTSA SISIFMLPMT PLILMLMT