NU2M_SHEEP
ID NU2M_SHEEP Reviewed; 347 AA.
AC O78748;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 2 {ECO:0000250|UniProtKB:P03891};
DE EC=7.1.1.2 {ECO:0000250|UniProtKB:P03891};
DE AltName: Full=NADH dehydrogenase subunit 2;
GN Name=MT-ND2 {ECO:0000250|UniProtKB:P03891}; Synonyms=MTND2, NADH2, ND2;
OS Ovis aries (Sheep).
OG Mitochondrion.
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Ovis.
OX NCBI_TaxID=9940;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Merinolandschaf {ECO:0000312|Proteomes:UP000002356}; TISSUE=Liver;
RX PubMed=9767689; DOI=10.1007/pl00006401;
RA Hiendleder S., Lewalski H., Wassmuth R., Janke A.;
RT "The complete mitochondrial DNA sequence of the domestic sheep (Ovis aries)
RT and comparison with the other major ovine haplotype.";
RL J. Mol. Evol. 47:441-448(1998).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) which catalyzes electron transfer from
CC NADH through the respiratory chain, using ubiquinone as an electron
CC acceptor. Essential for the catalytic activity and assembly of complex
CC I. {ECO:0000250|UniProtKB:P03891}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC Evidence={ECO:0000250|UniProtKB:P03891};
CC -!- SUBUNIT: Core subunit of respiratory chain NADH dehydrogenase (Complex
CC I) which is composed of 45 different subunits. Interacts with TMEM242
CC (By similarity). {ECO:0000250|UniProtKB:P03891,
CC ECO:0000250|UniProtKB:P03892}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:P03892}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the complex I subunit 2 family. {ECO:0000305}.
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DR EMBL; AF010406; AAD10097.1; -; Genomic_DNA.
DR PIR; T11051; T11051.
DR RefSeq; NP_008407.1; NC_001941.1.
DR PDB; 5LNK; EM; 3.90 A; N=1-347.
DR PDB; 6Q9B; EM; 3.90 A; D2=1-347.
DR PDB; 6QA9; EM; 4.10 A; D2=1-347.
DR PDB; 6QBX; EM; 4.20 A; D2=1-347.
DR PDB; 6QC2; EM; 4.20 A; D2=1-347.
DR PDB; 6QC3; EM; 4.20 A; D2=1-347.
DR PDB; 6QC4; EM; 4.60 A; D2=1-347.
DR PDB; 6QC5; EM; 4.30 A; D2=1-347.
DR PDB; 6QC6; EM; 4.10 A; D2=1-347.
DR PDB; 6QC7; EM; 4.40 A; D2=1-347.
DR PDB; 6QC8; EM; 4.20 A; D2=1-347.
DR PDB; 6QC9; EM; 5.70 A; D2=1-347.
DR PDB; 6QCA; EM; 6.20 A; D2=1-347.
DR PDB; 6QCF; EM; 6.50 A; D2=1-347.
DR PDB; 6ZKA; EM; 2.50 A; N=1-347.
DR PDB; 6ZKB; EM; 2.90 A; N=1-347.
DR PDB; 6ZKC; EM; 3.10 A; N=1-347.
DR PDB; 6ZKD; EM; 2.70 A; N=1-347.
DR PDB; 6ZKE; EM; 2.60 A; N=1-347.
DR PDB; 6ZKF; EM; 2.80 A; N=1-347.
DR PDB; 6ZKG; EM; 3.40 A; N=1-347.
DR PDB; 6ZKH; EM; 3.00 A; N=1-347.
DR PDB; 6ZKI; EM; 2.80 A; N=1-347.
DR PDB; 6ZKJ; EM; 3.00 A; N=1-347.
DR PDB; 6ZKK; EM; 3.70 A; N=1-347.
DR PDB; 6ZKL; EM; 3.10 A; N=1-347.
DR PDB; 6ZKM; EM; 2.80 A; N=1-347.
DR PDB; 6ZKN; EM; 2.90 A; N=1-347.
DR PDB; 6ZKO; EM; 3.80 A; N=1-347.
DR PDB; 6ZKP; EM; 3.20 A; N=1-347.
DR PDB; 6ZKQ; EM; 3.30 A; N=1-347.
DR PDB; 6ZKR; EM; 3.50 A; N=1-347.
DR PDB; 6ZKS; EM; 3.10 A; N=1-347.
DR PDB; 6ZKT; EM; 2.80 A; N=1-347.
DR PDB; 6ZKU; EM; 3.00 A; N=1-347.
DR PDB; 6ZKV; EM; 2.90 A; N=1-347.
DR PDBsum; 5LNK; -.
DR PDBsum; 6Q9B; -.
DR PDBsum; 6QA9; -.
DR PDBsum; 6QBX; -.
DR PDBsum; 6QC2; -.
DR PDBsum; 6QC3; -.
DR PDBsum; 6QC4; -.
DR PDBsum; 6QC5; -.
DR PDBsum; 6QC6; -.
DR PDBsum; 6QC7; -.
DR PDBsum; 6QC8; -.
DR PDBsum; 6QC9; -.
DR PDBsum; 6QCA; -.
DR PDBsum; 6QCF; -.
DR PDBsum; 6ZKA; -.
DR PDBsum; 6ZKB; -.
DR PDBsum; 6ZKC; -.
DR PDBsum; 6ZKD; -.
DR PDBsum; 6ZKE; -.
DR PDBsum; 6ZKF; -.
DR PDBsum; 6ZKG; -.
DR PDBsum; 6ZKH; -.
DR PDBsum; 6ZKI; -.
DR PDBsum; 6ZKJ; -.
DR PDBsum; 6ZKK; -.
DR PDBsum; 6ZKL; -.
DR PDBsum; 6ZKM; -.
DR PDBsum; 6ZKN; -.
DR PDBsum; 6ZKO; -.
DR PDBsum; 6ZKP; -.
DR PDBsum; 6ZKQ; -.
DR PDBsum; 6ZKR; -.
DR PDBsum; 6ZKS; -.
DR PDBsum; 6ZKT; -.
DR PDBsum; 6ZKU; -.
DR PDBsum; 6ZKV; -.
DR AlphaFoldDB; O78748; -.
DR SMR; O78748; -.
DR STRING; 9940.ENSOARP00000000002; -.
DR Ensembl; ENSOART00000000010; ENSOARP00000000002; ENSOARG00000000010.
DR Ensembl; ENSOART00020000011; ENSOARP00020000003; ENSOARG00020000011.
DR GeneID; 808250; -.
DR KEGG; oas:808250; -.
DR CTD; 4536; -.
DR eggNOG; KOG4668; Eukaryota.
DR HOGENOM; CLU_007100_1_3_1; -.
DR OMA; HFWVPEV; -.
DR OrthoDB; 1153818at2759; -.
DR Proteomes; UP000002356; Mitochondrion.
DR Bgee; ENSOARG00000000010; Expressed in cardiac muscle tissue of left auricle and 55 other tissues.
DR ExpressionAtlas; O78748; baseline.
DR GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; IEA:Ensembl.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; ISS:UniProtKB.
DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; ISS:UniProtKB.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR GO; GO:0072593; P:reactive oxygen species metabolic process; IEA:Ensembl.
DR InterPro; IPR010933; NADH_DH_su2_C.
DR InterPro; IPR003917; NADH_UbQ_OxRdtase_chain2.
DR InterPro; IPR001750; ND/Mrp_mem.
DR Pfam; PF06444; NADH_dehy_S2_C; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR PRINTS; PR01436; NADHDHGNASE2.
PE 1: Evidence at protein level;
KW 3D-structure; Electron transport; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; NAD; Reference proteome; Respiratory chain;
KW Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT CHAIN 1..347
FT /note="NADH-ubiquinone oxidoreductase chain 2"
FT /id="PRO_0000117638"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 25..45
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 67..87
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 96..116
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 122..142
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 145..165
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 237..257
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..294
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 325..345
FT /note="Helical"
FT /evidence="ECO:0000255"
FT HELIX 3..22
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 26..44
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 50..81
FT /evidence="ECO:0007829|PDB:6ZKA"
FT STRAND 85..88
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 92..106
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 112..114
FT /evidence="ECO:0007829|PDB:6ZKJ"
FT HELIX 115..121
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 124..144
FT /evidence="ECO:0007829|PDB:6ZKA"
FT TURN 145..148
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 151..170
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 175..192
FT /evidence="ECO:0007829|PDB:6ZKA"
FT TURN 193..196
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 198..222
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 227..230
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 231..235
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 238..251
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 260..270
FT /evidence="ECO:0007829|PDB:6ZKA"
FT TURN 271..274
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 277..300
FT /evidence="ECO:0007829|PDB:6ZKA"
FT TURN 301..303
FT /evidence="ECO:0007829|PDB:6ZKV"
FT STRAND 307..309
FT /evidence="ECO:0007829|PDB:6ZKE"
FT HELIX 311..314
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 326..334
FT /evidence="ECO:0007829|PDB:6ZKA"
FT HELIX 337..346
FT /evidence="ECO:0007829|PDB:6ZKA"
SQ SEQUENCE 347 AA; 39128 MW; 5202DF0A0D6C991B CRC64;
MNPIILIIIL MTVMLGTIIV MISTHWLLIW IGFEMNMLAI IPIMMKKHNP RATEASTKYF
LTQSTASMLL MMAIIINLMF SGQWTVMKLF NPMASMLMTM ALAMKLGMAP FHFWVPEVTQ
GIPLSSGLIL LTWQKLAPMS VLYQILPSIN LDLILTLSIL SITIGGWGGL NQTQLRKIMA
YSSIAHMGWM TAVLLYNPTM TLLNLIIYII MTSTMFTLFM ANSTTTTLSL SHTWNKAPIM
TILVLITLLS MGGLPPLSGF MPKWMIIQEM TKNDSIILPT LMAITALLNL YFYMRLTYST
ALTMFPSTNN MKMKWQFPTT KRMTLLPTMT VLSTMLLPLT PILSILE
