ID   NU2M_WICCA              Reviewed;         567 AA.
AC   P48906;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   30-AUG-2002, sequence version 2.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 2;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 2;
GN   Name=ND2;
OS   Wickerhamomyces canadensis (Yeast) (Pichia canadensis).
OG   Mitochondrion.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Phaffomycetaceae; Wickerhamomyces.
OX   NCBI_TaxID=1156965;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=21;
RX   PubMed=8536312; DOI=10.1007/bf00311880;
RA   Sekito T., Okamoto K., Kitano H., Yoshida K.;
RT   "The complete mitochondrial DNA sequence of Hansenula wingei reveals new
RT   characteristics of yeast mitochondria.";
RL   Curr. Genet. 28:39-53(1995).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane; Multi-pass membrane
CC       protein.
CC   -!- SIMILARITY: Belongs to the complex I subunit 2 family. {ECO:0000305}.
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DR   EMBL; D31785; BAA06573.2; -; Genomic_DNA.
DR   PIR; S58750; S58750.
DR   RefSeq; NP_038218.1; NC_001762.1.
DR   AlphaFoldDB; P48906; -.
DR   SMR; P48906; -.
DR   GeneID; 800551; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   Pfam; PF00361; Proton_antipo_M; 1.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   NAD; Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW   Transport; Ubiquinone.
FT   CHAIN           1..567
FT                   /note="NADH-ubiquinone oxidoreductase chain 2"
FT                   /id="PRO_0000117592"
FT   TRANSMEM        2..22
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        43..63
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        85..105
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        133..153
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        158..178
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        189..209
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        236..256
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        274..294
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        312..332
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        340..360
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        372..392
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        423..443
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        459..479
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        530..550
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   567 AA;  65299 MW;  8EA748C3E26FE290 CRC64;
     MLILSLFILI IYSSIINNID TINLLSSSNK IGWKLQNYNI IRIGIIIILY SLYIFKDISL
     SYIFNNYNNN NDLNIYIFND LYKLNIFNIY IIFLLLIVII SLLSINTTYL TKIQIKNNNN
     NISIGKSDNY IELNIYYISI IIFNIIGLIL LLTSNNLISI FISIELQSYS LYILTGIIPK
     SQKSGHNSLF YYLIGGIGSI IILYGISLLY YITSNIFINN INLIYSLDIY NINNNILIGW
     LFIIIGLLIK IGAAPMYNWS ILLYSNSNTI ITSYISLIPK ISILSYILLI ILNLYNLNNL
     NNLFNNNNNN NLIYILSIII ILSLIIGSIG GLTQIKIKNI LAYSGLLNIG YFLLIILSLI
     NNNNINSILA YIIYITQYCF NHISIFILLI IAIIYNNNYN NFITNKNLIY IYELNYIKNN
     RYLIFCLIII IGSFIGIPPL FGFYGKYYLL ISSINSNYLF LSLLLIISSI ISSIYYLYFL
     NITLFDNNNN KNNNNNNNNE SLLIPSFNII NDIKNNTSLS LSNKLKNNQV GNYITYILSS
     YILIILFNFI QWKNILKGTY LISILLF