NU2M_YARLI
ID NU2M_YARLI Reviewed; 469 AA.
AC Q9B6C8;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 92.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 2;
DE EC=7.1.1.2;
DE AltName: Full=NADH dehydrogenase subunit 2;
GN Name=ND2;
OS Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica).
OG Mitochondrion.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Dipodascaceae; Yarrowia.
OX NCBI_TaxID=284591;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 20460 / W29 / CBS 7504 / IFP29;
RX DOI=10.1002/cfg.72;
RA Kerscher S., Durstewitz G., Casaregola S., Gaillardin C., Brandt U.;
RT "The complete mitochondrial genome of Yarrowia lipolytica.";
RL Comp. Funct. Genomics 2:80-90(2001).
RN [2]
RP SUBUNIT.
RX PubMed=15282186; DOI=10.1016/j.bbabio.2004.04.019;
RA Abdrakhmanova A., Zickermann V., Bostina M., Radermacher M., Schagger H.,
RA Kerscher S., Brandt U.;
RT "Subunit composition of mitochondrial complex I from the yeast Yarrowia
RT lipolytica.";
RL Biochim. Biophys. Acta 1658:148-156(2004).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBUNIT: Complex I is composed of 37 different subunits.
CC {ECO:0000269|PubMed:15282186}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane; Multi-pass membrane
CC protein.
CC -!- SIMILARITY: Belongs to the complex I subunit 2 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AJ307410; CAC28115.2; -; Genomic_DNA.
DR RefSeq; NP_075445.2; NC_002659.1.
DR PDB; 6GCS; EM; 4.32 A; 2=1-469.
DR PDB; 6H8K; X-ray; 3.79 A; 2=1-415.
DR PDB; 6RFQ; EM; 3.30 A; 2=1-469.
DR PDB; 6RFR; EM; 3.20 A; 2=1-469.
DR PDB; 6RFS; EM; 4.04 A; 2=1-469.
DR PDB; 6Y79; EM; 3.20 A; 2=1-469.
DR PDB; 6YJ4; EM; 2.70 A; N=1-469.
DR PDBsum; 6GCS; -.
DR PDBsum; 6H8K; -.
DR PDBsum; 6RFQ; -.
DR PDBsum; 6RFR; -.
DR PDBsum; 6RFS; -.
DR PDBsum; 6Y79; -.
DR PDBsum; 6YJ4; -.
DR AlphaFoldDB; Q9B6C8; -.
DR SMR; Q9B6C8; -.
DR DIP; DIP-61437N; -.
DR IntAct; Q9B6C8; 2.
DR STRING; 284591.Q9B6C8; -.
DR GeneID; 802601; -.
DR KEGG; yli:YalifMp28; -.
DR InParanoid; Q9B6C8; -.
DR Proteomes; UP000001300; Mitochondrion.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR InterPro; IPR001750; ND/Mrp_mem.
DR Pfam; PF00361; Proton_antipo_M; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Electron transport; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; NAD; Reference proteome; Respiratory chain;
KW Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT CHAIN 1..469
FT /note="NADH-ubiquinone oxidoreductase chain 2"
FT /id="PRO_0000117649"
FT TRANSMEM 27..47
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 125..145
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 153..173
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..217
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 230..250
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 257..277
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 284..304
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 317..337
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..381
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 397..417
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 441..461
FT /note="Helical"
FT /evidence="ECO:0000255"
FT HELIX 2..14
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 18..20
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 21..38
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 40..46
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 47..49
FT /evidence="ECO:0007829|PDB:6Y79"
FT STRAND 52..54
FT /evidence="ECO:0007829|PDB:6YJ4"
FT TURN 55..58
FT /evidence="ECO:0007829|PDB:6YJ4"
FT STRAND 59..61
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 63..84
FT /evidence="ECO:0007829|PDB:6YJ4"
FT STRAND 90..92
FT /evidence="ECO:0007829|PDB:6RFR"
FT HELIX 93..96
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 103..118
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 123..142
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 148..179
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 184..186
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 189..194
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 198..209
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 210..212
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 215..217
FT /evidence="ECO:0007829|PDB:6RFR"
FT HELIX 219..227
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 230..236
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 239..250
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 258..273
FT /evidence="ECO:0007829|PDB:6YJ4"
FT STRAND 275..277
FT /evidence="ECO:0007829|PDB:6RFR"
FT HELIX 281..297
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 298..300
FT /evidence="ECO:0007829|PDB:6YJ4"
FT STRAND 301..303
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 306..329
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 330..333
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 347..350
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 353..356
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 358..372
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 378..392
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 397..420
FT /evidence="ECO:0007829|PDB:6YJ4"
FT STRAND 425..430
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 437..452
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 453..456
FT /evidence="ECO:0007829|PDB:6YJ4"
FT HELIX 457..467
FT /evidence="ECO:0007829|PDB:6YJ4"
SQ SEQUENCE 469 AA; 53332 MW; 42489BC23DD4158A CRC64;
MLILAIISLI TFVSMSKLSD NRAIIRLINI YLILVLVLDS FLYLLFLNNQ TYTVMGELLI
FNSFTFYIDM LIYFIMIVIS SLYGYNLYNN NLYKTLFEPK KELIILFLIN ILGALLIVHS
NDFITLFVAI ELQSYSIYLI TAIYNSSYKA SKASMLYFFM GGILSILIAY SINTYYSVLN
SYTLHSLDSL IINTLDLNLI LIALSLGLLF KIGIAPLHKW LISIYENTPI LITIYISLIP
KISILSYLVL SNISINSLVI SILAILTLLV GSVGGLLQIK IKRLLAFSGL TNAGYMMLLL
LLNNNEFSYL YYITQYSISH LAIFMIIIFS IYYINYINNQ YNPIIYVNQL KGLIHDNAYL
VLSMAIVVFS FIGIPPLLGF FGKLNILMSI LNNGYYFISI VLIVASLISA LYYLYLLNVS
IQDKNNILIN SNETVSSVLS YILSSLIILI TFGFIYNSLI IDIFNVYFN
