NU3M_BRANA
ID NU3M_BRANA Reviewed; 119 AA.
AC P68160; O24083; P48908; Q6YSP3;
DT 25-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2004, sequence version 1.
DT 25-MAY-2022, entry version 69.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 3;
DE EC=7.1.1.2;
DE AltName: Full=NADH dehydrogenase subunit 3;
GN Name=ND3; Synonyms=NAD3;
OS Brassica napus (Rape).
OG Mitochondrion.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3708;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TISSUE=Leaf;
RA Ye F., Bernhardt J., Abel W.O.;
RL Submitted (JAN-1992) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Handa H., Shimizu T., Nakajima K., Naito T.;
RL Submitted (JUL-1993) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP RNA EDITING.
RX PubMed=9799366; DOI=10.1007/s002940050402;
RA Itani K., Handa H.;
RT "Rapeseed mitochondrial ccb206, a gene involved in cytochrome c biogenesis,
RT is co-transcribed with the nad3 and rps12 genes: organization,
RT transcription, and RNA editing of the nad3/rps12/ccb206 locus.";
RL Curr. Genet. 34:318-325(1998).
RN [4]
RP RNA EDITING.
RC TISSUE=Leaf;
RX PubMed=14530439; DOI=10.1093/nar/gkg795;
RA Handa H.;
RT "The complete nucleotide sequence and RNA editing content of the
RT mitochondrial genome of rapeseed (Brassica napus L.): comparative analysis
RT of the mitochondrial genomes of rapeseed and Arabidopsis thaliana.";
RL Nucleic Acids Res. 31:5907-5916(2003).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- RNA EDITING: Modified_positions=3 {ECO:0000269|PubMed:14530439,
CC ECO:0000269|PubMed:9799366}, 9 {ECO:0000269|PubMed:14530439,
CC ECO:0000269|PubMed:9799366}, 28 {ECO:0000269|PubMed:14530439,
CC ECO:0000269|PubMed:9799366}, 50 {ECO:0000269|PubMed:14530439,
CC ECO:0000269|PubMed:9799366}, 71 {ECO:0000269|PubMed:14530439,
CC ECO:0000269|PubMed:9799366}, 84 {ECO:0000269|PubMed:14530439,
CC ECO:0000269|PubMed:9799366}, 85 {ECO:0000269|PubMed:14530439,
CC ECO:0000269|PubMed:9799366}, 116 {ECO:0000269|PubMed:14530439,
CC ECO:0000269|PubMed:9799366}, 118 {ECO:0000269|PubMed:14530439,
CC ECO:0000269|PubMed:9799366};
CC -!- SIMILARITY: Belongs to the complex I subunit 3 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC98895.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; X63654; CAA45193.1; ALT_SEQ; Genomic_DNA.
DR EMBL; D13697; BAA02856.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP006444; BAC98895.1; ALT_INIT; Genomic_DNA.
DR PIR; T09501; T09501.
DR PIR; T09519; T09519.
DR RefSeq; YP_717146.1; NC_008285.1.
DR AlphaFoldDB; P68160; -.
DR SMR; P68160; -.
DR GeneID; 4237970; -.
DR KEGG; bna:4237970; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR Gene3D; 1.20.58.1610; -; 1.
DR HAMAP; MF_01394; NDH1_NuoA; 1.
DR InterPro; IPR023043; NAD(P)H_OxRDtase_bac/plastid.
DR InterPro; IPR000440; NADH_UbQ/plastoQ_OxRdtase_su3.
DR InterPro; IPR038430; NDAH_ubi_oxred_su3_sf.
DR PANTHER; PTHR11058; PTHR11058; 1.
DR Pfam; PF00507; Oxidored_q4; 1.
PE 2: Evidence at transcript level;
KW Electron transport; Membrane; Mitochondrion; NAD; Respiratory chain;
KW RNA editing; Translocase; Transmembrane; Transmembrane helix; Transport;
KW Ubiquinone.
FT CHAIN 1..119
FT /note="NADH-ubiquinone oxidoreductase chain 3"
FT /id="PRO_0000117719"
FT TRANSMEM 8..28
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 63..83
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..108
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 16
FT /note="L -> P (in Ref. 1; CAA45193)"
FT /evidence="ECO:0000305"
FT CONFLICT 22
FT /note="L -> P (in Ref. 1; CAA45193)"
FT /evidence="ECO:0000305"
FT CONFLICT 47
FT /note="F -> S (in Ref. 1; CAA45193)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 119 AA; 13935 MW; 0BD4C8F07C94B8F8 CRC64;
MMLEFAPIFI YLVISLLVSL ILLGVPFLFA SNSSTYPEKL SAYECGFDPF GDARSRFDIR
FYLVSILFLI FDLEVTFFFP WAVSLNKIDL FGFWSMMAFL FILTIGFLYE WKRGALDWE
