ID   NU3M_METSE              Reviewed;         118 AA.
AC   Q35100;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 3;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 3;
GN   Name=ND3;
OS   Metridium senile (Brown sea anemone) (Frilled sea anemone).
OG   Mitochondrion.
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC   Nynantheae; Metridiidae; Metridium.
OX   NCBI_TaxID=6116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=White morph;
RX   PubMed=8643626; DOI=10.1073/pnas.93.11.5619;
RA   Beagley C.T., Okada N.A., Wolstenholme D.R.;
RT   "Two mitochondrial group I introns in a metazoan, the sea anemone Metridium
RT   senile: one intron contains genes for subunits 1 and 3 of NADH
RT   dehydrogenase.";
RL   Proc. Natl. Acad. Sci. U.S.A. 93:5619-5623(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=White morph;
RA   Beagley C.T., Okimoto R., Wolstenholme D.R.;
RL   Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 3 family. {ECO:0000305}.
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DR   EMBL; U36784; AAC47101.1; -; Genomic_DNA.
DR   EMBL; AF000023; AAC04637.1; -; Genomic_DNA.
DR   PIR; T11891; T11891.
DR   RefSeq; NP_009260.1; NC_000933.1.
DR   AlphaFoldDB; Q35100; -.
DR   SMR; Q35100; -.
DR   GeneID; 808775; -.
DR   CTD; 4537; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   Gene3D; 1.20.58.1610; -; 1.
DR   HAMAP; MF_01394; NDH1_NuoA; 1.
DR   InterPro; IPR023043; NAD(P)H_OxRDtase_bac/plastid.
DR   InterPro; IPR000440; NADH_UbQ/plastoQ_OxRdtase_su3.
DR   InterPro; IPR038430; NDAH_ubi_oxred_su3_sf.
DR   PANTHER; PTHR11058; PTHR11058; 1.
DR   Pfam; PF00507; Oxidored_q4; 1.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; NAD; Respiratory chain;
KW   Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT   CHAIN           1..118
FT                   /note="NADH-ubiquinone oxidoreductase chain 3"
FT                   /id="PRO_0000117763"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        60..80
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        87..107
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   118 AA;  13517 MW;  3DBB968006083B71 CRC64;
     MYTEFYGILV LLIFSVVLSA IISGASYILG DKQPDREKVS AYECGFDPFG TPGRPFSIRF
     FLIGILFLIF DLEISFLFPW CVVCNQVFPF GYWTMIVFLA VLTLGLVYEW LKGGLEWE