NU3M_MOUSE
ID NU3M_MOUSE Reviewed; 115 AA.
AC P03899; Q35039; Q35040; Q9MD73;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 16-NOV-2001, sequence version 3.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 3 {ECO:0000305};
DE EC=7.1.1.2 {ECO:0000250|UniProtKB:P03897};
DE AltName: Full=NADH dehydrogenase subunit 3;
GN Name=mt-Nd3 {ECO:0000312|MGI:MGI:102499}; Synonyms=Nd3;
OS Mus musculus (Mouse).
OG Mitochondrion.
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7332926; DOI=10.1016/0092-8674(81)90300-7;
RA Bibb M.J., van Etten R.A., Wright C.T., Walberg M.W., Clayton D.A.;
RT "Sequence and gene organization of mouse mitochondrial DNA.";
RL Cell 26:167-180(1981).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8022788; DOI=10.1073/pnas.91.14.6364;
RA Nachman M.W., Boyer S.N., Aquadro C.F.;
RT "Nonneutral evolution at the mitochondrial NADH dehydrogenase subunit 3
RT gene in mice.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:6364-6368(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=AKR/J, C3H/He, SAMP1, SAMP8, and SAMR1; TISSUE=Liver;
RX PubMed=11535650; DOI=10.1093/jhered/92.4.352;
RA Mizutani J., Chiba T., Tanaka M., Higuchi K., Mori M.;
RT "Unique mutations in mitochondrial DNA of senescence-accelerated mouse
RT (SAM) strains.";
RL J. Hered. 92:352-355(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=12954771; DOI=10.1093/nar/gkg739;
RA Bayona-Bafaluy M.P., Acin-Perez R., Mullikin J.C., Park J.S.,
RA Moreno-Loshuertos R., Hu P., Perez-Martos A., Fernandez-Silva P., Bai Y.,
RA Enriquez J.A.;
RT "Revisiting the mouse mitochondrial DNA sequence.";
RL Nucleic Acids Res. 31:5349-5355(2003).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) which catalyzes electron transfer from
CC NADH through the respiratory chain, using ubiquinone as an electron
CC acceptor. Essential for the catalytic activity of complex I.
CC {ECO:0000250|UniProtKB:P03897}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC Evidence={ECO:0000250|UniProtKB:P03897};
CC -!- SUBUNIT: Core subunit of respiratory chain NADH dehydrogenase (Complex
CC I) which is composed of 45 different subunits. Interacts with TMEM186.
CC Interacts with TMEM242 (By similarity). {ECO:0000250|UniProtKB:P03897}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:P03898}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the complex I subunit 3 family. {ECO:0000305}.
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DR EMBL; J01420; AAB48651.1; -; Genomic_DNA.
DR EMBL; V00711; CAA24086.1; -; Genomic_DNA.
DR EMBL; U09637; AAA19254.2; -; Genomic_DNA.
DR EMBL; U09638; AAA19255.2; -; Genomic_DNA.
DR EMBL; AB042432; BAA95623.1; -; Genomic_DNA.
DR EMBL; AB042523; BAA95660.1; -; Genomic_DNA.
DR EMBL; AB042524; BAA95647.1; -; Genomic_DNA.
DR EMBL; AB042809; BAA95802.1; -; Genomic_DNA.
DR EMBL; AB049357; BAB13808.1; -; Genomic_DNA.
DR EMBL; AY172335; AAN85129.1; -; Genomic_DNA.
DR PIR; A55746; A55746.
DR PIR; B55746; QXMS3M.
DR RefSeq; NP_904335.1; NC_005089.1.
DR RefSeq; YP_220557.1; NC_006914.1.
DR PDB; 6G2J; EM; 3.30 A; A=1-115.
DR PDB; 6G72; EM; 3.90 A; A=1-115.
DR PDB; 6ZR2; EM; 3.10 A; A=1-115.
DR PDB; 6ZTQ; EM; 3.00 A; A=1-115.
DR PDB; 7AK5; EM; 3.17 A; A=1-115.
DR PDB; 7AK6; EM; 3.82 A; A=1-115.
DR PDB; 7B93; EM; 3.04 A; A=1-115.
DR PDB; 7PSA; EM; 3.40 A; A=1-115.
DR PDBsum; 6G2J; -.
DR PDBsum; 6G72; -.
DR PDBsum; 6ZR2; -.
DR PDBsum; 6ZTQ; -.
DR PDBsum; 7AK5; -.
DR PDBsum; 7AK6; -.
DR PDBsum; 7B93; -.
DR PDBsum; 7PSA; -.
DR AlphaFoldDB; P03899; -.
DR SMR; P03899; -.
DR BioGRID; 201549; 2.
DR ComplexPortal; CPX-266; Mitochondrial respiratory chain complex I.
DR CORUM; P03899; -.
DR IntAct; P03899; 1.
DR STRING; 10090.ENSMUSP00000080998; -.
DR iPTMnet; P03899; -.
DR PhosphoSitePlus; P03899; -.
DR SwissPalm; P03899; -.
DR EPD; P03899; -.
DR jPOST; P03899; -.
DR PaxDb; P03899; -.
DR PeptideAtlas; P03899; -.
DR PRIDE; P03899; -.
DR ProteomicsDB; 295541; -.
DR Antibodypedia; 68603; 127 antibodies from 24 providers.
DR Ensembl; ENSMUST00000082411; ENSMUSP00000080998; ENSMUSG00000064360.
DR GeneID; 17718; -.
DR GeneID; 3338896; -.
DR KEGG; mmu:17718; -.
DR CTD; 4537; -.
DR MGI; MGI:102499; mt-Nd3.
DR VEuPathDB; HostDB:ENSMUSG00000064360; -.
DR eggNOG; KOG4662; Eukaryota.
DR GeneTree; ENSGT00390000011605; -.
DR HOGENOM; CLU_119549_3_1_1; -.
DR InParanoid; P03899; -.
DR OMA; RFPVKYY; -.
DR OrthoDB; 1605459at2759; -.
DR PhylomeDB; P03899; -.
DR Reactome; R-MMU-611105; Respiratory electron transport.
DR Reactome; R-MMU-6799198; Complex I biogenesis.
DR ChiTaRS; mt-Nd3; mouse.
DR PRO; PR:P03899; -.
DR Proteomes; UP000000589; Mitochondrion.
DR RNAct; P03899; protein.
DR Bgee; ENSMUSG00000064360; Expressed in hypothalamus and 60 other tissues.
DR ExpressionAtlas; P03899; baseline and differential.
DR GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; ISO:MGI.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; ISS:UniProtKB.
DR GO; GO:0009060; P:aerobic respiration; IC:ComplexPortal.
DR GO; GO:0071385; P:cellular response to glucocorticoid stimulus; ISO:MGI.
DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; ISS:UniProtKB.
DR GO; GO:0042776; P:proton motive force-driven mitochondrial ATP synthesis; IC:ComplexPortal.
DR GO; GO:0009725; P:response to hormone; ISO:MGI.
DR GO; GO:0009642; P:response to light intensity; IEA:Ensembl.
DR GO; GO:0006979; P:response to oxidative stress; ISO:MGI.
DR Gene3D; 1.20.58.1610; -; 1.
DR InterPro; IPR000440; NADH_UbQ/plastoQ_OxRdtase_su3.
DR InterPro; IPR038430; NDAH_ubi_oxred_su3_sf.
DR PANTHER; PTHR11058; PTHR11058; 1.
DR Pfam; PF00507; Oxidored_q4; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Electron transport; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; NAD; Reference proteome; Respiratory chain;
KW Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT CHAIN 1..115
FT /note="NADH-ubiquinone oxidoreductase chain 3"
FT /id="PRO_0000117765"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 55..75
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 86..106
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 16
FT /note="T -> A (in Ref. 2; AAA19255)"
FT /evidence="ECO:0000305"
FT CONFLICT 33
FT /note="Missing (in Ref. 1; AAB48651/CAA24086)"
FT /evidence="ECO:0000305"
FT HELIX 2..23
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 31..33
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT TURN 38..40
FT /evidence="ECO:0007829|PDB:6G2J"
FT STRAND 47..49
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 56..72
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 75..78
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT HELIX 86..107
FT /evidence="ECO:0007829|PDB:6ZTQ"
FT STRAND 110..112
FT /evidence="ECO:0007829|PDB:6ZTQ"
SQ SEQUENCE 115 AA; 13219 MW; 6925F293601CB391 CRC64;
MNLYTVIFIN ILLSLTLILV AFWLPQMNLY SEKANPYECG FDPTSSARLP FSMKFFLVAI
TFLLFDLEIA LLLPLPWAIQ TIKTSTMMIM AFILVTILSL GLAYEWTQKG LEWTE
