NU3M_PINSY
ID NU3M_PINSY Reviewed; 118 AA.
AC Q36664;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 25-MAY-2022, entry version 71.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 3;
DE EC=7.1.1.2;
DE AltName: Full=NADH dehydrogenase subunit 3;
GN Name=NAD3;
OS Pinus sylvestris (Scotch pine).
OG Mitochondrion.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Pinus;
OC Pinus subgen. Pinus.
OX NCBI_TaxID=3349;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND RNA EDITING.
RC TISSUE=Cotyledon;
RX PubMed=8575014; DOI=10.1007/bf00310810;
RA Karpinska B., Karpinski S., Hallgren J.E.;
RT "The genes encoding subunit 3 of NADH dehydrogenase and ribosomal protein
RT S12 are co-transcribed and edited in Pinus sylvestris (L.) mitochondria.";
RL Curr. Genet. 28:423-428(1995).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- RNA EDITING: Modified_positions=15 {ECO:0000269|PubMed:8575014}, 27
CC {ECO:0000269|PubMed:8575014}, 30 {ECO:0000269|PubMed:8575014}, 42
CC {ECO:0000269|PubMed:8575014}, 46 {ECO:0000269|PubMed:8575014}, 49
CC {ECO:0000269|PubMed:8575014}, 62 {ECO:0000269|PubMed:8575014}, 64
CC {ECO:0000269|PubMed:8575014}, 66 {ECO:0000269|PubMed:8575014}, 70
CC {ECO:0000269|PubMed:8575014}, 72 {ECO:0000269|PubMed:8575014}, 77
CC {ECO:0000269|PubMed:8575014}, 78 {ECO:0000269|PubMed:8575014}, 80
CC {ECO:0000269|PubMed:8575014}, 83 {ECO:0000269|PubMed:8575014}, 89
CC {ECO:0000269|PubMed:8575014}, 92 {ECO:0000269|PubMed:8575014}, 93
CC {ECO:0000269|PubMed:8575014}, 99 {ECO:0000269|PubMed:8575014}, 102
CC {ECO:0000269|PubMed:8575014}, 107 {ECO:0000269|PubMed:8575014}, 108
CC {ECO:0000269|PubMed:8575014}, 115 {ECO:0000269|PubMed:8575014}, 117
CC {ECO:0000269|PubMed:8575014};
CC -!- SIMILARITY: Belongs to the complex I subunit 3 family. {ECO:0000305}.
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DR EMBL; X86217; CAA60117.1; ALT_SEQ; Genomic_DNA.
DR PIR; S60468; S60468.
DR AlphaFoldDB; Q36664; -.
DR SMR; Q36664; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR Gene3D; 1.20.58.1610; -; 1.
DR HAMAP; MF_01394; NDH1_NuoA; 1.
DR InterPro; IPR023043; NAD(P)H_OxRDtase_bac/plastid.
DR InterPro; IPR000440; NADH_UbQ/plastoQ_OxRdtase_su3.
DR InterPro; IPR038430; NDAH_ubi_oxred_su3_sf.
DR PANTHER; PTHR11058; PTHR11058; 1.
DR Pfam; PF00507; Oxidored_q4; 1.
PE 2: Evidence at transcript level;
KW Electron transport; Membrane; Mitochondrion; NAD; Respiratory chain;
KW RNA editing; Translocase; Transmembrane; Transmembrane helix; Transport;
KW Ubiquinone.
FT CHAIN 1..118
FT /note="NADH-ubiquinone oxidoreductase chain 3"
FT /id="PRO_0000117806"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 118 AA; 13669 MW; 25AC6B8F8F9F1F52 CRC64;
MSEFAPICIY LVISLLVCLI PLGVPFLFAS NGSTYPEKLS AYECGFDPFG DARSRFDIRF
YLVSILFIIF DLEVTFFFPW AVSLNKIDLF GFWSMMVFLL ILTIGFLYEW KKGALDWE
