ID   NU3M_RAPSA              Reviewed;         119 AA.
AC   P68159; P48908;
DT   25-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2004, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 3;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 3;
GN   Name=ND3; Synonyms=NAD3;
OS   Raphanus sativus (Radish) (Raphanus raphanistrum var. sativus).
OG   Mitochondrion.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Raphanus.
OX   NCBI_TaxID=3726;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. CRGC15, and cv. Scarlet Knight;
RX   PubMed=8662196; DOI=10.1007/bf02426961;
RA   Rankin C.T., Cutright M.T., Makaroff C.A.;
RT   "Characterization of the radish mitochondrial nad3/rps12 locus: analysis of
RT   recombination repeats and RNA editing.";
RL   Curr. Genet. 29:564-571(1996).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- RNA EDITING: Modified_positions=3 {ECO:0000250}, 9 {ECO:0000250}, 28
CC       {ECO:0000250}, 50 {ECO:0000250}, 71 {ECO:0000250}, 84 {ECO:0000250}, 85
CC       {ECO:0000250}, 116 {ECO:0000250}, 118 {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the complex I subunit 3 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB18648.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=AAB18650.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; U43506; AAB18648.1; ALT_INIT; Genomic_DNA.
DR   EMBL; U43507; AAB18650.1; ALT_INIT; Genomic_DNA.
DR   PIR; S70000; S70000.
DR   RefSeq; YP_006665996.1; NC_018551.1.
DR   AlphaFoldDB; P68159; -.
DR   SMR; P68159; -.
DR   GeneID; 13630152; -.
DR   KEGG; rsz:13630152; -.
DR   OrthoDB; 1605459at2759; -.
DR   Proteomes; UP000504610; Mitochondrion MT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   Gene3D; 1.20.58.1610; -; 1.
DR   HAMAP; MF_01394; NDH1_NuoA; 1.
DR   InterPro; IPR023043; NAD(P)H_OxRDtase_bac/plastid.
DR   InterPro; IPR000440; NADH_UbQ/plastoQ_OxRdtase_su3.
DR   InterPro; IPR038430; NDAH_ubi_oxred_su3_sf.
DR   PANTHER; PTHR11058; PTHR11058; 1.
DR   Pfam; PF00507; Oxidored_q4; 1.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; NAD; Reference proteome;
KW   Respiratory chain; RNA editing; Translocase; Transmembrane;
KW   Transmembrane helix; Transport; Ubiquinone.
FT   CHAIN           1..119
FT                   /note="NADH-ubiquinone oxidoreductase chain 3"
FT                   /id="PRO_0000117817"
FT   TRANSMEM        8..28
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        63..83
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        88..108
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   119 AA;  13935 MW;  0BD4C8F07C94B8F8 CRC64;
     MMLEFAPIFI YLVISLLVSL ILLGVPFLFA SNSSTYPEKL SAYECGFDPF GDARSRFDIR
     FYLVSILFLI FDLEVTFFFP WAVSLNKIDL FGFWSMMAFL FILTIGFLYE WKRGALDWE