NU4C_SPIOL
ID NU4C_SPIOL Reviewed; 500 AA.
AC Q9M3J0;
DT 29-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT 05-DEC-2001, sequence version 2.
DT 25-MAY-2022, entry version 86.
DE RecName: Full=NAD(P)H-quinone oxidoreductase chain 4, chloroplastic;
DE EC=7.1.1.-;
DE AltName: Full=NAD(P)H dehydrogenase, chain 4;
DE AltName: Full=NADH-plastoquinone oxidoreductase chain 4;
GN Name=ndhD;
OS Spinacia oleracea (Spinach).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC Caryophyllales; Chenopodiaceae; Chenopodioideae; Anserineae; Spinacia.
OX NCBI_TaxID=3562;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Geant d'hiver, and cv. Monatol;
RX PubMed=11292076; DOI=10.1023/a:1006478403810;
RA Schmitz-Linneweber C., Maier R.M., Alcaraz J.-P., Cottet A., Herrmann R.G.,
RA Mache R.;
RT "The plastid chromosome of spinach (Spinacia oleracea): complete nucleotide
RT sequence and gene organization.";
RL Plant Mol. Biol. 45:307-315(2001).
RN [2]
RP RNA EDITING OF INITIATOR CODON.
RX PubMed=8076816; DOI=10.1016/0378-1119(94)90290-9;
RA Neckermann K., Zeltz P., Igloi G.L., Koessel H., Maier R.M.;
RT "The role of RNA editing in conservation of start codons in chloroplast
RT genomes.";
RL Gene 146:177-182(1994).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a plastoquinone + (n+1) H(+)(in) + NADH = a plastoquinol + n
CC H(+)(out) + NAD(+); Xref=Rhea:RHEA:42608, Rhea:RHEA-COMP:9561,
CC Rhea:RHEA-COMP:9562, ChEBI:CHEBI:15378, ChEBI:CHEBI:17757,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:62192;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a plastoquinone + (n+1) H(+)(in) + NADPH = a plastoquinol + n
CC H(+)(out) + NADP(+); Xref=Rhea:RHEA:42612, Rhea:RHEA-COMP:9561,
CC Rhea:RHEA-COMP:9562, ChEBI:CHEBI:15378, ChEBI:CHEBI:17757,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:62192;
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
CC -!- RNA EDITING: Modified_positions=1 {ECO:0000269|PubMed:8076816};
CC Note=The initiator methionine is created by RNA editing.;
CC -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB88784.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AJ400848; CAB88784.1; ALT_INIT; Genomic_DNA.
DR RefSeq; NP_054988.2; NC_002202.1.
DR AlphaFoldDB; Q9M3J0; -.
DR SMR; Q9M3J0; -.
DR STRING; 3562.Q9M3J0; -.
DR GeneID; 2715588; -.
DR KEGG; soe:2715588; -.
DR OrthoDB; 996580at2759; -.
DR Proteomes; UP000054095; Chloroplast.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:InterPro.
DR GO; GO:0048039; F:ubiquinone binding; IBA:GO_Central.
DR GO; GO:0009060; P:aerobic respiration; IBA:GO_Central.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR GO; GO:0015990; P:electron transport coupled proton transport; IBA:GO_Central.
DR HAMAP; MF_00491; NDH1_NuoM; 1.
DR InterPro; IPR022997; NADH_Q_OxRdtase_chain4.
DR InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR InterPro; IPR001750; ND/Mrp_mem.
DR PANTHER; PTHR43507; PTHR43507; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR PRINTS; PR01437; NUOXDRDTASE4.
DR TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Membrane; NAD; NADP; Plastid; Plastoquinone; Quinone;
KW Reference proteome; RNA editing; Thylakoid; Translocase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..500
FT /note="NAD(P)H-quinone oxidoreductase chain 4,
FT chloroplastic"
FT /id="PRO_0000118030"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 35..55
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 111..131
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 134..154
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 208..228
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 242..262
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 272..292
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 305..325
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 330..350
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 386..406
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 416..436
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 500 AA; 56187 MW; 5090AE36D7DA5FF0 CRC64;
MNSFPWLTTI VVLPIFAGSL IFLFPHRGNK VIRWYTICIS MIELLLMTYV FFYHFQPDDP
LIQLVEDYKW INFFDFHWRL GIDGLSIGPI LLTGFITTLA TLAAWPVTRN SQLFHFLMLA
MYSAQIGLFS SRDLLLFFIM WELELIPVYL LLSMWGGKKR LYSATKFILY TAGGSIFLLM
GVLGVGLYGS NEPTLNLETL VNQSYPVALE IIFYIGFFIA FAVKLPIIPL HTWLPDTHGE
AHYSTCMLLA GILLKMGAYG LVRINMELLP HAHSIFSPWL MIIGTMQIIY AASTSPGQRN
LKKRIAYSSV SHMGFIIIGI SSITDTGLNG AILQIISHGF IGAALFFLAG TSYDRIRLVY
LDEMGGIAIP MPKIFTLFSS FSMASLALPG MSGFIAELIV FFGLITSQKY LLIPKLLITF
GMAIGMILTP IYLLSMSRQM FYGYKLFNIS NSSFFDSGPR ELFVSTSIFL PVIGIGVYPD
LVLSLSVEKV EAILSNYFYR
