NU4LC_ARATH
ID NU4LC_ARATH Reviewed; 101 AA.
AC P26289;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=NAD(P)H-quinone oxidoreductase subunit 4L, chloroplastic {ECO:0000255|HAMAP-Rule:MF_01456};
DE EC=7.1.1.- {ECO:0000255|HAMAP-Rule:MF_01456};
DE AltName: Full=NAD(P)H dehydrogenase subunit 4L {ECO:0000255|HAMAP-Rule:MF_01456};
DE AltName: Full=NADH-plastoquinone oxidoreductase subunit 4L {ECO:0000255|HAMAP-Rule:MF_01456};
GN Name=ndhE {ECO:0000255|HAMAP-Rule:MF_01456}; OrderedLocusNames=AtCg01070;
OS Arabidopsis thaliana (Mouse-ear cress).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10574454; DOI=10.1093/dnares/6.5.283;
RA Sato S., Nakamura Y., Kaneko T., Asamizu E., Tabata S.;
RT "Complete structure of the chloroplast genome of Arabidopsis thaliana.";
RL DNA Res. 6:283-290(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 21-101.
RX PubMed=1907869; DOI=10.1007/bf00023416;
RA Anderson S.L., McIntosh L.;
RT "Partial conservation of the 5' ndhE-psaC-ndhD 3' gene arrangement of
RT chloroplasts in the cyanobacterium Synechocystis sp. PCC 6803: implications
RT for NDH-D function in cyanobacteria and chloroplasts.";
RL Plant Mol. Biol. 16:487-499(1991).
CC -!- FUNCTION: NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN
CC and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain
CC and possibly in a chloroplast respiratory chain. The immediate electron
CC acceptor for the enzyme in this species is believed to be
CC plastoquinone. Couples the redox reaction to proton translocation, and
CC thus conserves the redox energy in a proton gradient.
CC {ECO:0000255|HAMAP-Rule:MF_01456}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a plastoquinone + (n+1) H(+)(in) + NADH = a plastoquinol + n
CC H(+)(out) + NAD(+); Xref=Rhea:RHEA:42608, Rhea:RHEA-COMP:9561,
CC Rhea:RHEA-COMP:9562, ChEBI:CHEBI:15378, ChEBI:CHEBI:17757,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:62192;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01456};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a plastoquinone + (n+1) H(+)(in) + NADPH = a plastoquinol + n
CC H(+)(out) + NADP(+); Xref=Rhea:RHEA:42612, Rhea:RHEA-COMP:9561,
CC Rhea:RHEA-COMP:9562, ChEBI:CHEBI:15378, ChEBI:CHEBI:17757,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:62192;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01456};
CC -!- SUBUNIT: NDH is composed of at least 16 different subunits, 5 of which
CC are encoded in the nucleus. {ECO:0000255|HAMAP-Rule:MF_01456}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000255|HAMAP-Rule:MF_01456}; Multi-pass membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_01456}.
CC -!- SIMILARITY: Belongs to the complex I subunit 4L family.
CC {ECO:0000255|HAMAP-Rule:MF_01456}.
CC -!- CAUTION: Was originally thought to originate from Synechocystis
CC PCC6803. {ECO:0000305|PubMed:1907869}.
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DR EMBL; AP000423; BAA84439.1; -; Genomic_DNA.
DR EMBL; X53842; CAA37835.1; -; Genomic_DNA.
DR RefSeq; NP_051111.1; NC_000932.1.
DR PDB; 7WFF; EM; 3.59 A; E=1-101.
DR PDB; 7WG5; EM; 3.89 A; E=1-101.
DR PDBsum; 7WFF; -.
DR PDBsum; 7WG5; -.
DR AlphaFoldDB; P26289; -.
DR SMR; P26289; -.
DR STRING; 3702.ATCG01070.1; -.
DR TCDB; 3.D.1.8.1; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR PaxDb; P26289; -.
DR ProteomicsDB; 249429; -.
DR EnsemblPlants; ATCG01070.1; ATCG01070.1; ATCG01070.
DR GeneID; 844741; -.
DR Gramene; ATCG01070.1; ATCG01070.1; ATCG01070.
DR KEGG; ath:ArthCp076; -.
DR Araport; ATCG01070; -.
DR TAIR; locus:504954730; ATCG01070.
DR eggNOG; KOG4669; Eukaryota.
DR HOGENOM; CLU_144724_1_1_1; -.
DR InParanoid; P26289; -.
DR OMA; FDVWLSR; -.
DR OrthoDB; 1621687at2759; -.
DR BioCyc; ARA:ATCG01070-MON; -.
DR PRO; PR:P26289; -.
DR Proteomes; UP000006548; Chloroplast.
DR ExpressionAtlas; P26289; baseline and differential.
DR Genevisible; P26289; AT.
DR GO; GO:0009507; C:chloroplast; TAS:TAIR.
DR GO; GO:0009534; C:chloroplast thylakoid; HDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IBA:GO_Central.
DR GO; GO:0003959; F:NADPH dehydrogenase activity; TAS:TAIR.
DR GO; GO:0048038; F:quinone binding; IEA:UniProtKB-KW.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR GO; GO:0019684; P:photosynthesis, light reaction; IEA:UniProtKB-UniRule.
DR GO; GO:0006744; P:ubiquinone biosynthetic process; TAS:TAIR.
DR HAMAP; MF_01456; NDH1_NuoK; 1.
DR InterPro; IPR001133; NADH_UbQ_OxRdtase_chain4L/K.
DR InterPro; IPR039428; NUOK/Mnh_C1-like.
DR PANTHER; PTHR11434; PTHR11434; 1.
DR Pfam; PF00420; Oxidored_q2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Membrane; NAD; NADP; Plastid; Plastoquinone;
KW Quinone; Reference proteome; Thylakoid; Translocase; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..101
FT /note="NAD(P)H-quinone oxidoreductase subunit 4L,
FT chloroplastic"
FT /id="PRO_0000118504"
FT TRANSMEM 2..22
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01456"
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01456"
FT TRANSMEM 61..81
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01456"
FT CONFLICT 21
FT /note="L -> R (in Ref. 2; CAA37835)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 101 AA; 11288 MW; 5FF69CC1E382A214 CRC64;
MILEHVLVLS AYLFLIGLYG LITSRNMVRA LMCLELILNA VNMNFVTFSD FFDNSQLKGE
IFCIFVIAIA AAEAAIGLAI VSSIYRNRKS IRINQSTLLN K
