NU4M_AEDAE
ID NU4M_AEDAE Reviewed; 447 AA.
AC Q1HR20;
DT 02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 13-JUN-2006, sequence version 1.
DT 25-MAY-2022, entry version 68.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 4 {ECO:0000250|UniProtKB:P03910};
DE EC=7.1.1.2;
DE AltName: Full=NADH dehydrogenase subunit 4 {ECO:0000250|UniProtKB:P03910};
GN Name=mt:ND4 {ECO:0000250|UniProtKB:P18931};
GN Synonyms=ND4 {ECO:0000312|EMBL:ABY51631.1};
OS Aedes aegypti (Yellowfever mosquito) (Culex aegypti).
OG Mitochondrion {ECO:0000312|EMBL:ABF18307.1}.
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC Culicinae; Aedini; Aedes; Stegomyia.
OX NCBI_TaxID=7159;
RN [1] {ECO:0000312|EMBL:ABF18307.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Black-eyed Liverpool; TISSUE=Salivary gland;
RX PubMed=17204158; DOI=10.1186/1471-2164-8-6;
RA Ribeiro J.M.C., Arca B., Lombardo F., Calvo E., Phan V.M., Chandra P.K.,
RA Wikel S.K.;
RT "An annotated catalogue of salivary gland transcripts in the adult female
RT mosquito, Aedes aegypti.";
RL BMC Genomics 8:6-6(2007).
RN [2] {ECO:0000312|EMBL:ABY51631.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LVPib12 {ECO:0000312|EMBL:ABY51631.1};
RA Lobo N.F., Lovin D., DeBruyn B., Puiu D., Shumway M., Haas B., Nene V.,
RA Severson D.W.;
RT "The mitochondrial genome of the Yellow fever mosquito - Aedes aegypti.";
RL Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone (By
CC similarity). {ECO:0000250|UniProtKB:P03910}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000255}.
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DR EMBL; DQ440274; ABF18307.1; -; mRNA.
DR EMBL; EU352212; ABY51631.1; -; Genomic_DNA.
DR RefSeq; YP_001649170.1; NC_010241.1.
DR AlphaFoldDB; Q1HR20; -.
DR SMR; Q1HR20; -.
DR STRING; 7159.AAEL018680-PA; -.
DR VEuPathDB; VectorBase:AAEL018680; -.
DR eggNOG; KOG4845; Eukaryota.
DR HOGENOM; CLU_007100_4_0_1; -.
DR InParanoid; Q1HR20; -.
DR OrthoDB; 996580at2759; -.
DR Proteomes; UP000008820; Unplaced.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR InterPro; IPR000260; NADH4_N.
DR InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR InterPro; IPR001750; ND/Mrp_mem.
DR PANTHER; PTHR43507; PTHR43507; 1.
DR Pfam; PF01059; Oxidored_q5_N; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR PRINTS; PR01437; NUOXDRDTASE4.
PE 2: Evidence at transcript level;
KW Electron transport; Membrane; Mitochondrion; NAD; Reference proteome;
KW Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW Transport; Ubiquinone.
FT CHAIN 1..447
FT /note="NADH-ubiquinone oxidoreductase chain 4"
FT /id="PRO_0000347268"
FT TRANSMEM 28..48
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 110..130
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 141..161
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 183..203
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 213..233
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 246..266
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 273..293
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 301..321
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 343..365
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 380..400
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 409..431
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 447 AA; 51632 MW; E22F7921B4AAD81B CRC64;
MLKFIFMIMF IFFLFFKKNI YWMVQNLIFL ATCLFMIKIS SNYYFCDISY YFGMDMISYG
LILLSFWICG LMLMASEGVV RYNNYVNLFL FMIVFLLLML IFTFSSMSMF MFYLFFESSL
IPTLFLILGW GYQPERLQAG IYLLFYTLLA SLPLLIGIFY IKNDNYTMNF IMLSYKNLYN
LDFLYLCMIF AFLVKMPMFL VHLWLPKAHV EAPVSGSMIL AGVLLKLGGY GLLRVFSLMQ
VLGMKFNYIW ISISLIGGVL VSLICLWQMD LKALIAYSSV AHMGIVLSGL MTMTYWGLNG
SYTLMIAHGL CSSGLFCLAN ISYERMGSRS LLINKGMLNF MPSLSLWWFL LCSGNMAAPP
TLNLLGEISL LNSIVSWSWL TMISLAFLSF FSAAYTLYLF AYSQHGKIYS GVYFFSSGTT
REFLVLMLHW LPLNLLIMKS NFCMLWI
