ID   NU4M_ANOAR              Reviewed;         221 AA.
AC   P51898;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 4;
DE   Flags: Fragment;
GN   Name=ND4;
OS   Anopheles arabiensis (Mosquito).
OG   Mitochondrion.
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC   Anophelinae; Anopheles.
OX   NCBI_TaxID=7173;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Arzag, BAL, Brefet, Chil, G3, GMAL, Squad, and Zulu;
RX   PubMed=8041714; DOI=10.1073/pnas.91.15.6885;
RA   Besansky N.J., Powell J.R., Caccone A., Hamm D.M., Scott J.A.,
RA   Collins F.H.;
RT   "Molecular phylogeny of the Anopheles gambiae complex suggests genetic
RT   introgression between principal malaria vectors.";
RL   Proc. Natl. Acad. Sci. U.S.A. 91:6885-6888(1994).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR   EMBL; U10123; AAB51640.1; -; Genomic_DNA.
DR   EMBL; U10124; AAB51630.1; -; Genomic_DNA.
DR   EMBL; U10125; AAB51632.1; -; Genomic_DNA.
DR   EMBL; U10126; AAB51634.1; -; Genomic_DNA.
DR   EMBL; U10129; AAB51642.1; -; Genomic_DNA.
DR   EMBL; U10131; AAB51646.1; -; Genomic_DNA.
DR   EMBL; U10132; AAB51648.1; -; Genomic_DNA.
DR   EMBL; U10133; AAB51650.1; -; Genomic_DNA.
DR   PIR; T30245; T30245.
DR   AlphaFoldDB; P51898; -.
DR   SMR; P51898; -.
DR   VEuPathDB; VectorBase:AARA21_005044; -.
DR   Proteomes; UP000075840; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR   InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   PANTHER; PTHR43507; PTHR43507; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   PRINTS; PR01437; NUOXDRDTASE4.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; NAD; Respiratory chain;
KW   Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT   CHAIN           <1..221
FT                   /note="NADH-ubiquinone oxidoreductase chain 4"
FT                   /id="PRO_0000117885"
FT   TRANSMEM        20..40
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        45..65
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        78..98
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        121..141
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        170..190
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   VARIANT         221
FT                   /note="S -> N (in strain: Arzag)"
FT   NON_TER         1
SQ   SEQUENCE   221 AA;  24798 MW;  E5678DDF515A48A7 CRC64;
     SLVGGVLISL VCLRQTDLKA LIAYSSVAHM GIVLSGLLTM TYWGLTGSYA LMIAHGLCSS
     GLFCLANISY ERMGSRSLLI NKGLLNFMPT LSLWWFLLCS GNMAAPPTLN LLGEISLLNS
     IVSWSWITMI MLSFLSFFSA AYSLYLFAYS QHGKIYSGVY FFSVGTTREF LLLMLHWLPL
     NLLILKSNFC MLWIYLNSLK KMLICGVNDM KLFILDRELF S