ID   NU4M_ARBLI              Reviewed;         457 AA.
AC   P34941;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 4;
GN   Name=ND4;
OS   Arbacia lixula (Black urchin) (Echinus lixula).
OG   Mitochondrion.
OC   Eukaryota; Metazoa; Echinodermata; Eleutherozoa; Echinozoa; Echinoidea;
OC   Euechinoidea; Echinacea; Arbacioida; Arbaciidae; Arbacia.
OX   NCBI_TaxID=7640;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1681410; DOI=10.1093/oxfordjournals.molbev.a040661;
RA   de Giorgi C., Lanave C., Musci M.D., Saccone C.;
RT   "Mitochondrial DNA in the sea urchin Arbacia lixula: evolutionary
RT   inferences from nucleotide sequence analysis.";
RL   Mol. Biol. Evol. 8:515-529(1991).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR   EMBL; M74839; AAA98046.1; -; Genomic_DNA.
DR   EMBL; X53727; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; P34941; -.
DR   SMR; P34941; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR   InterPro; IPR000260; NADH4_N.
DR   InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   PANTHER; PTHR43507; PTHR43507; 1.
DR   Pfam; PF01059; Oxidored_q5_N; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   PRINTS; PR01437; NUOXDRDTASE4.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; NAD; Respiratory chain;
KW   Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT   CHAIN           1..457
FT                   /note="NADH-ubiquinone oxidoreductase chain 4"
FT                   /id="PRO_0000117890"
FT   TRANSMEM        22..42
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        59..79
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        95..115
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        116..136
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        148..168
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        191..211
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        223..243
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        257..277
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        282..302
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        309..329
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        350..370
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        433..453
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   457 AA;  51082 MW;  9628DB24B48A934B CRC64;
     MITLIFISIG MTLTSFVVSK NNLWPVTILQ SAFLSIAAIA LINNHWISKW HNFINSVDSM
     QLPLLVLSCW LTPLALIASK GHLNNLPITN QRTFIVLVII ITTSLIITFS SLELILFYIA
     FETTLVPTLI LITRWGALME RFQAGLYFIF YTLFGSLPLL ISLIALYFSS NSLSIPNVEL
     VWLTTNSSTS LTVWWLLSIL AFLVKMPIYG FHLWLPKAHV EAPVAGSMIL AAILLKLGGY
     GLMRLISLFS TTSLNFSSLP LVVFCCWGAL VTSIICIRQT DLKALIAYSS VGHMSIVAAG
     VFSQTIWGIN GALMLMIAHG LVSSALFALA NTMYERSGTR TLVITRGMKL ILPLSTFWWL
     IMCAANLGFP YSNLIGEILY ISWYGWSIWC SYFSNYNCVW GVYSLMIFQV SQQGPSSHFL
     LNVPTSFSRE HLLFLLHLLP LLLIIPTPNL VLISWLK