ID   NU4M_ASCSU              Reviewed;         409 AA.
AC   P24880;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 2.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 4;
GN   Name=ND4;
OS   Ascaris suum (Pig roundworm) (Ascaris lumbricoides).
OG   Mitochondrion.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Spirurina; Ascaridomorpha; Ascaridoidea; Ascarididae; Ascaris.
OX   NCBI_TaxID=6253;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Body wall muscle, and Egg;
RX   PubMed=1551572; DOI=10.1093/genetics/130.3.471;
RA   Okimoto R., Macfarlane J.L., Clary D.O., Wolstenholme D.R.;
RT   "The mitochondrial genomes of two nematodes, Caenorhabditis elegans and
RT   Ascaris suum.";
RL   Genetics 130:471-498(1992).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR   EMBL; X54253; CAA38170.1; -; Genomic_DNA.
DR   PIR; S26021; S26021.
DR   RefSeq; NP_006948.1; NC_001327.1.
DR   AlphaFoldDB; P24880; -.
DR   SMR; P24880; -.
DR   GeneID; 807666; -.
DR   CTD; 4538; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR   InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   PANTHER; PTHR43507; PTHR43507; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   PRINTS; PR01437; NUOXDRDTASE4.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; NAD; Respiratory chain;
KW   Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT   CHAIN           1..409
FT                   /note="NADH-ubiquinone oxidoreductase chain 4"
FT                   /id="PRO_0000117893"
FT   TRANSMEM        9..29
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        44..64
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        68..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        90..110
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        125..145
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        160..180
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        194..214
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        221..241
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        246..268
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        273..295
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        305..325
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        352..372
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        389..409
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   409 AA;  47065 MW;  A4B135C1CA5587B6 CRC64;
     MLDILLFSLY FFFEPVLFFF FMVVFGFVAL NNYSWLGCFY FFDSFSFILL IVMSLFILGV
     VLLSESNFML LLLSEVLVVV CVFFFVPSNV ILMYMYFELS MFPILVMILG YGSQIEKINS
     SYYLIFYAAL CSFPFLFVYF KSFFFISLVY FDFNLSWEMV FVLSLSFMMK FPVYFLHLWL
     PKAHVEAPTT ASMLLAGLLL KLGTAGFLRI LGCLSFVHNN VWIVLAFLGM ILASFCCMFQ
     SDAKALAAYS SITHMSFVLM ALVFIIMSGK TGGVILMLAH GYTSTLMFYL VGEFYHVSGS
     RMVYYMSSFF GSGMIMALLF AVVFLSNMGT PPSLSFLSEF IVISSSLNMM KFSFWVLFVY
     FFSAFYYSIY LLTSSVMGKG YVNFSIWNVG FSVPLVFMMY NIFWMSVFF