NU4M_AVACL
ID NU4M_AVACL Reviewed; 459 AA.
AC A8DQK9; A8DQK5;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2007, sequence version 1.
DT 25-MAY-2022, entry version 42.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE EC=7.1.1.2 {ECO:0000250|UniProtKB:P03905};
DE AltName: Full=NADH dehydrogenase subunit 4;
GN Name=MT-ND4; Synonyms=MTND4, NADH4, ND4;
OS Avahi cleesei (Cleese's woolly lemur) (Bemaraha woolly lemur).
OG Mitochondrion.
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Strepsirrhini; Lemuriformes;
OC Indriidae; Avahi.
OX NCBI_TaxID=402244;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Isolate BEMA12, Isolate BEMA13, Isolate BEMA14, Isolate BEMA8, and
RC Isolate BEMA9;
RA Andriantompohavana R., Lei R., Zaonarivelo J.R., Engberg S.E.,
RA Nalanirina G., McGuire S.M., Shore G.D., Andrianasolo J., Herrington K.,
RA Brenneman R.A., Louis E.E. Jr.;
RT "Molecular phylogeny and taxonomic revision of the woolly lemurs, genus
RT Avahi (primates: lemuriformes).";
RL Spec. Publ. Mus. Tex. Tech. Univ. 51:1-64(2007).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) which catalyzes electron transfer from
CC NADH through the respiratory chain, using ubiquinone as an electron
CC acceptor. Essential for the catalytic activity and assembly of complex
CC I. {ECO:0000250|UniProtKB:P03905}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC Evidence={ECO:0000250|UniProtKB:P03905};
CC -!- SUBUNIT: Core subunit of respiratory chain NADH dehydrogenase (Complex
CC I) which is composed of 45 different subunits.
CC {ECO:0000250|UniProtKB:P03910}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:P03910}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR EMBL; DQ856113; ABI54997.1; -; Genomic_DNA.
DR EMBL; DQ856114; ABI55001.1; -; Genomic_DNA.
DR EMBL; DQ856115; ABI55005.1; -; Genomic_DNA.
DR EMBL; DQ856116; ABI55009.1; -; Genomic_DNA.
DR EMBL; DQ856117; ABI55013.1; -; Genomic_DNA.
DR AlphaFoldDB; A8DQK9; -.
DR SMR; A8DQK9; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; ISS:UniProtKB.
DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; ISS:UniProtKB.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR InterPro; IPR000260; NADH4_N.
DR InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR InterPro; IPR001750; ND/Mrp_mem.
DR PANTHER; PTHR43507; PTHR43507; 1.
DR Pfam; PF01059; Oxidored_q5_N; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR PRINTS; PR01437; NUOXDRDTASE4.
DR TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE 3: Inferred from homology;
KW Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW NAD; Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW Transport; Ubiquinone.
FT CHAIN 1..459
FT /note="NADH-ubiquinone oxidoreductase chain 4"
FT /id="PRO_0000323384"
FT TRANSMEM 22..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..111
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 194..214
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 224..244
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 257..277
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 284..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..330
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 342..362
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 391..411
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VARIANT 19
FT /note="K -> N (in strain: Isolate BEMA8)"
SQ SEQUENCE 459 AA; 51929 MW; AE1F0008CCAEDE79 CRC64;
MLKIIIPTIM LFPVTWYSKN SMIWINTTSH SLMISLMGLL LLNHPSNNSN NFSLNFFSDP
LSSPLLMLTM WLLPLMIMAS QHHLAKEPWF RKKSYLSMLI TLQMFLIMTF MATELILFYI
LFEATLIPTL IIITRWGNQT ERLNAGMYFL FYTLTGSLPL LVALIYLQSS MGSLNLLTIN
LWLKELPNSW STNLLWMACI MAFMVKMPLY GLHLWLPKAH VEAPIAGSMV LAAVLLKLGG
YGMMRITIIL DPTTKSMAYP FLMLCLWGMI MTSSICLRQT DLKSLIAYSS VSHMALVIVA
ILVQTPLGFM GATALMIAHG LTSSMLFCLA NSNYERIHSR TMLMARGLQT LLPLMTTWWL
LASLNNLALP PSINLIGELL VTITSFSWSN ITVILIGLNM LITALYSLYM LITTQRGKLT
YYLHNLNPSL TRENTLMSMH MLPLLFLTLN PKIILGPTF
