NU4M_BRAFL
ID NU4M_BRAFL Reviewed; 452 AA.
AC O47423;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 25-MAY-2022, entry version 90.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE EC=7.1.1.2;
DE AltName: Full=NADH dehydrogenase subunit 4;
GN Name=ND4; Synonyms=NAD4, NADH4;
OS Branchiostoma floridae (Florida lancelet) (Amphioxus).
OG Mitochondrion.
OC Eukaryota; Metazoa; Chordata; Cephalochordata; Leptocardii; Amphioxiformes;
OC Branchiostomidae; Branchiostoma.
OX NCBI_TaxID=7739;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S238N-H82 {ECO:0000312|Proteomes:UP000001554};
RX PubMed=10331267; DOI=10.1093/oxfordjournals.molbev.a026122;
RA Boore J.L., Daehler L.L., Brown W.M.;
RT "Complete sequence, gene arrangement, and genetic code of mitochondrial DNA
RT of the cephalochordate Branchiostoma floridae (Amphioxus).";
RL Mol. Biol. Evol. 16:410-418(1999).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR EMBL; AF098298; AAB87992.1; -; Genomic_DNA.
DR RefSeq; NP_007764.1; NC_000834.1.
DR AlphaFoldDB; O47423; -.
DR SMR; O47423; -.
DR GeneID; 808733; -.
DR KEGG; bfo:808733; -.
DR CTD; 4538; -.
DR InParanoid; O47423; -.
DR OMA; ITRWGNQ; -.
DR Proteomes; UP000001554; Mitochondrion.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR GO; GO:0048039; F:ubiquinone binding; IBA:GO_Central.
DR GO; GO:0009060; P:aerobic respiration; IBA:GO_Central.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR GO; GO:0015990; P:electron transport coupled proton transport; IBA:GO_Central.
DR InterPro; IPR000260; NADH4_N.
DR InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR InterPro; IPR001750; ND/Mrp_mem.
DR PANTHER; PTHR43507; PTHR43507; 1.
DR Pfam; PF01059; Oxidored_q5_N; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR PRINTS; PR01437; NUOXDRDTASE4.
DR TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE 3: Inferred from homology;
KW Electron transport; Membrane; Mitochondrion; NAD; Reference proteome;
KW Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW Transport; Ubiquinone.
FT CHAIN 1..452
FT /note="NADH-ubiquinone oxidoreductase chain 4"
FT /id="PRO_0000117908"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 29..49
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..110
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..136
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 144..164
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..272
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..304
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 309..331
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 345..365
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 390..410
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 432..452
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 452 AA; 49914 MW; A1F4C8F26FA077F9 CRC64;
MNHLVLGYVG LVIGVIVTKK SMVWRVGQVG SVLLMLPATV LVNMNMTISN VSYMMTSDFV
SLGLTVLSIW LLPLMLLASQ QHMVSESLIY QRVFVGCQVF LTGALVLAFM ASDLLLFYIA
FESTLLPTLM LITRWGAQKE RYQAGTYFMF FTLVGSLPLL ICLIGQYQMV GSLALDLSYE
GVFQLSYLVN FWWVGCILAF LVKLPLYGVH LWLPKAHVEA PIAGSMVLAG VLLKLGGYGM
MRVSLMWGAT AMLSSEVFLA LALWGIVVMG GICLRQTDLK SLIAYSSVGH MALVVGGVLT
GVAWGYNGAM VLMIAHGLVS SCLFCLANLW YERSSTRNLS GSRGLIMIFP LISSGWFLMS
LMNMALPPAI NLFGELVAMV ALYNWSPYSI VYMSLGAVLT AAYSLYLFGM SQWGNTMKNY
KNLYTITSRE YLLTTLHLVP AIYLIFYLGL MF
