ID   NU4M_BRALA              Reviewed;         452 AA.
AC   O79421;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 4;
GN   Name=ND4; Synonyms=NAD4, NADH4;
OS   Branchiostoma lanceolatum (Common lancelet) (Amphioxus lanceolatum).
OG   Mitochondrion.
OC   Eukaryota; Metazoa; Chordata; Cephalochordata; Leptocardii; Amphioxiformes;
OC   Branchiostomidae; Branchiostoma.
OX   NCBI_TaxID=7740;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9628930; DOI=10.1093/nar/26.13.3279;
RA   Spruyt N., Delarbre C., Gachelin G., Laudet V.;
RT   "Complete sequence of the amphioxus (Branchiostoma lanceolatum)
RT   mitochondrial genome: relations to vertebrates.";
RL   Nucleic Acids Res. 26:3279-3285(1998).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR   EMBL; Y16474; CAA76256.1; -; Genomic_DNA.
DR   PIR; C71391; C71391.
DR   RefSeq; NP_007546.1; NC_001912.1.
DR   AlphaFoldDB; O79421; -.
DR   SMR; O79421; -.
DR   GeneID; 808213; -.
DR   CTD; 4538; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR   InterPro; IPR000260; NADH4_N.
DR   InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   PANTHER; PTHR43507; PTHR43507; 1.
DR   Pfam; PF01059; Oxidored_q5_N; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   PRINTS; PR01437; NUOXDRDTASE4.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; NAD; Respiratory chain;
KW   Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT   CHAIN           1..452
FT                   /note="NADH-ubiquinone oxidoreductase chain 4"
FT                   /id="PRO_0000117909"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        29..49
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        59..79
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        88..110
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        114..136
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        144..164
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        182..202
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        221..241
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        252..272
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        282..304
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        309..331
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        345..365
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        390..410
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        432..452
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   452 AA;  49940 MW;  4067DB59EC184C7A CRC64;
     MNHLVLGYVG LVIGVIVTKK SMVWRVGQVG SVLLMLPATV LVNMNMTISN VSYMMTSDFV
     SLGLTVLSIW LLPLMLLASQ QHMVSESLIY QRVFVGCQVF LTGALVLAFM ASDLLLFYIA
     FESTLLPTLM LITRWGAQKE RYQAGTYFMF FTLVGSLPLL ICLIGQYQMV GSLALDLSYE
     GVFQLSYLVN FWWVGCILAF LVKLPLYGVH LWLPKAHVEA PIAGSMVLAG VLLKLGGYGM
     MRVSLMWGAT AMLSSEVFLA LALWGIVVMG GICLRQTDLK SLIAYSSVGH MALVVGGVLT
     GVAWGYNGAM VLMIAHGLVS SCLFCLANLW YERSSTRNLS GSRGLIMIFP LISLGWFLMS
     LMNMALPPAI NLFGELVAMV ALYNWSPYSI VYMSLGAVLT AAYSLYLFGM SQWGNTMKNY
     KNLYTITSRE YLLTTLHLVP AIYLIFYLGL MF