ID   NU4M_CANAL              Reviewed;         464 AA.
AC   Q9B8C8;
DT   16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 2.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 4;
GN   Name=NAD4; OrderedLocusNames=CM_00440W {ECO:0000312|CGD:CAL0000192075};
GN   ORFNames=CaalfMp14;
OS   Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OG   Mitochondrion.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX   NCBI_TaxID=237561;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=11208783; DOI=10.1128/jb.183.3.865-872.2001;
RA   Anderson J.B., Wickens C., Khan M., Cowen L.E., Federspiel N.A., Jones T.,
RA   Kohn L.M.;
RT   "Infrequent genetic exchange and recombination in the mitochondrial genome
RT   of Candida albicans.";
RL   J. Bacteriol. 183:865-872(2001).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR   EMBL; AF285261; AAG59598.2; -; Genomic_DNA.
DR   RefSeq; NP_075041.2; NC_002653.1.
DR   AlphaFoldDB; Q9B8C8; -.
DR   SMR; Q9B8C8; -.
DR   STRING; 237561.Q9B8C8; -.
DR   GeneID; 802562; -.
DR   KEGG; cal:CaalfMp14; -.
DR   CGD; CAL0000192075; NAD4.
DR   VEuPathDB; FungiDB:CM_00440W; -.
DR   InParanoid; Q9B8C8; -.
DR   Proteomes; UP000000559; Mitochondrion.
DR   GO; GO:0005747; C:mitochondrial respiratory chain complex I; ISS:CGD.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; ISS:CGD.
DR   GO; GO:0048039; F:ubiquinone binding; IBA:GO_Central.
DR   GO; GO:0009060; P:aerobic respiration; IBA:GO_Central.
DR   GO; GO:0015990; P:electron transport coupled proton transport; IBA:GO_Central.
DR   GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; ISS:CGD.
DR   InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR   InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   PANTHER; PTHR43507; PTHR43507; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   PRINTS; PR01437; NUOXDRDTASE4.
DR   TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; NAD; Reference proteome;
KW   Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW   Transport; Ubiquinone.
FT   CHAIN           1..464
FT                   /note="NADH-ubiquinone oxidoreductase chain 4"
FT                   /id="PRO_0000356878"
FT   TRANSMEM        18..38
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        54..74
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        79..99
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        102..122
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        131..151
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        168..188
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        207..227
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        239..259
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        266..286
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        297..317
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        332..352
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        375..395
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        420..440
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   464 AA;  51142 MW;  11036F46FBD72C08 CRC64;
     MTLIYILILA GVSIFSRLLP TYGKGLILVA SILVVLPTMT DWEEVGIYYT SDGIADIFIL
     LTAYLLPLSI IANWNNIRSL LYFELILNLG VILLINFMCQ DMLSFYVYFE ISLAPLFILI
     GLYGANNRDK AADYILIYTL FSSLFMLLAI GTYEVLIGNT DYQAVSLVVL STDLQCILFL
     CISAGIMVKT PLVPVHTWLP VVHSESPLAG SMLLAGVILK LAVYAIIRLI IPTLSDATVL
     YTPVVYVICA ITIIYTSIIT LRQTDLKVIV AYSSISHMAV CILGILSNSL AGINGSLILS
     LAHGFVSPAL FIIVGGILYD RYHHRLIYYY QGLLTYMPIL AIYLLILSFS NIGTPLTVNF
     IGELLSLTGA IGRSTILGCI SAFSVLLSAA YMLKVTNRLT GGIRTPYTAL TSDCTYRESL
     LMIALIIPTL WYGLYPNGII NMIWQGSKLL YIFVITQYNV IGNI