ID   NU4M_CARAU              Reviewed;         460 AA.
AC   O78687;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 4;
GN   Name=MT-ND4; Synonyms=MTND4, NADH4, ND4;
OS   Carassius auratus (Goldfish).
OG   Mitochondrion.
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Cyprinidae; Cyprininae; Carassius.
OX   NCBI_TaxID=7957;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=AZ3 / Langsdorfi; TISSUE=Oocyte;
RA   Murakami M., Yamashita Y., Fujitani H.;
RT   "The complete sequence of mitochondrial genome from a gynogenetic triploid
RT   'ginbuna' (Carassius auratus langsdorfi).";
RL   Zool. Sci. 15:335-337(1998).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR   EMBL; AB006953; BAA31247.1; -; Genomic_DNA.
DR   RefSeq; NP_008597.1; NC_002079.1.
DR   AlphaFoldDB; O78687; -.
DR   SMR; O78687; -.
DR   Ensembl; ENSCART00000000029; ENSCARP00000000011; ENSCARG00000000029.
DR   GeneID; 808421; -.
DR   CTD; 4538; -.
DR   Proteomes; UP000515129; Mitochondrion MT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR   InterPro; IPR000260; NADH4_N.
DR   InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR   InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   PANTHER; PTHR43507; PTHR43507; 1.
DR   Pfam; PF01059; Oxidored_q5_N; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   PRINTS; PR01437; NUOXDRDTASE4.
DR   TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; NAD; Reference proteome;
KW   Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW   Transport; Ubiquinone.
FT   CHAIN           1..460
FT                   /note="NADH-ubiquinone oxidoreductase chain 4"
FT                   /id="PRO_0000117915"
FT   TRANSMEM        20..42
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        61..81
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        93..113
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        114..134
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        148..168
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        195..215
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        225..245
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        258..278
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        285..304
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        308..330
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        351..371
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        394..414
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   460 AA;  51554 MW;  3316E91DC0472A95 CRC64;
     MLKVLIPTIM LFPTIWLTSP KWLWTTTTAH SLLIASISLM WFKWTSETGW TSSNTYLATD
     PLSTPLLVLT CWLLPLMILA SQNHINPEPI SRQRLYITLL ASLQTFLIMA FGATEIIMFY
     IMFEATLIPT LIIITRWGNQ TERLNAGTYF LFYTLAGSLP LLVALLLLQQ STGTLSMLVL
     QYSQPLQLNS WGHMIWWAAC LIAFLVKMPL YGVHLWLPKA HVEAPVAGSM VLAAVLLKLG
     GYGMMRMMVM LDPLSKELAY PFIILALWGI IMTGSICLRQ TDLKSLIAYS SVSHMGLVAG
     GILIQTPWGF SGAIILMIAH GLVSSALFCL ANTAYERTHS RTMILARGLQ IIFPLTAVWW
     FIANLANLAL PPLPNLMGEL MIITTLFNWS PWTILLTGLG TLITAGYSLY MFLMSQRGPT
     PNHITGLQPF HTREHLLMTL HLIPVILLVT KPELMWGWCY