ID   NU4M_CERCA              Reviewed;         446 AA.
AC   Q34048; Q34053;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 2.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 4;
GN   Name=ND4;
OS   Ceratitis capitata (Mediterranean fruit fly) (Tephritis capitata).
OG   Mitochondrion.
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Tephritoidea;
OC   Tephritidae; Ceratitis; Ceratitis.
OX   NCBI_TaxID=7213;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Guatemala laboratory colony, and Hawaii laboratory colony;
RX   PubMed=7742977; DOI=10.1111/j.1365-2583.1995.tb00008.x;
RA   Gasparich G.E., Sheppard W.S., Han H.Y., McPheron B.A., Steck G.J.;
RT   "Analysis of mitochondrial DNA and development of PCR-based diagnostic
RT   molecular markers for Mediterranean fruit fly (Ceratitis capitata)
RT   populations.";
RL   Insect Mol. Biol. 4:61-67(1995).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR   EMBL; U12925; AAA85797.1; -; Genomic_DNA.
DR   EMBL; U12924; AAA85796.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q34048; -.
DR   SMR; Q34048; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR   InterPro; IPR000260; NADH4_N.
DR   InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   PANTHER; PTHR43507; PTHR43507; 1.
DR   Pfam; PF01059; Oxidored_q5_N; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   PRINTS; PR01437; NUOXDRDTASE4.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; NAD; Respiratory chain;
KW   Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT   CHAIN           1..446
FT                   /note="NADH-ubiquinone oxidoreductase chain 4"
FT                   /id="PRO_0000117917"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        28..48
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        56..76
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        88..105
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        109..131
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        141..161
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        182..202
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        218..238
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        245..265
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        272..292
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        297..317
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        330..350
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        373..393
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        426..446
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   446 AA;  50968 MW;  E9AAAC06796897FD CRC64;
     MLKLILMMIF ISPICFMQGL FWMVHNLLFV ITFVLILMNF CLNYFVNISY FMGFDVLSYG
     LILLSFWICS LMIVASESVN KYNNYKNLFL FNVLMLLIFL VLTFSSMNLF MFYLFFESSL
     IPTLFLILGW GYQPERLQAG VYLLFYTLLV SLPLLVGIFY LYKNCNTMNF YLLSNYMFNC
     NLLYLAMILA FLVKMPMFLV HLWLPKAHVE APVSGSMILA GIMLKLGGYG LLAVFSFLQL
     IGLKYNYIWV SISLIGGVLI SLVCLRQTDL KALIAYSSVA HMGIVLGGLM TLSYWGLSGS
     YTLMIGHGLC SSGLFCLANI TYERLGSRSL LINKGLLNFM PSMTLWWFLL SVCNMAAPPS
     LNLLGEISLL NSIVSWSWLT MISLSFLSFF SAAYTLYLYA YSQHGKIFSG VYSFSGGNIR
     EYFLLFLHWF PLNLLILKSE VCLFWI