ID   NU4M_CERSI              Reviewed;         459 AA.
AC   O03204;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE            EC=7.1.1.2 {ECO:0000250|UniProtKB:P03905};
DE   AltName: Full=NADH dehydrogenase subunit 4;
GN   Name=MT-ND4; Synonyms=MTND4, NADH4, ND4;
OS   Ceratotherium simum (White rhinoceros) (Square-lipped rhinoceros).
OG   Mitochondrion.
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Perissodactyla; Rhinocerotidae; Ceratotherium.
OX   NCBI_TaxID=9807;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9126560; DOI=10.1006/mpev.1996.0385;
RA   Xu X., Arnason U.;
RT   "The complete mitochondrial DNA sequence of the white rhinoceros,
RT   Ceratotherium simum, and comparison with the mtDNA sequence of the Indian
RT   rhinoceros, Rhinoceros unicornis.";
RL   Mol. Phylogenet. Evol. 7:189-194(1997).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) which catalyzes electron transfer from
CC       NADH through the respiratory chain, using ubiquinone as an electron
CC       acceptor. Essential for the catalytic activity and assembly of complex
CC       I. {ECO:0000250|UniProtKB:P03905}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC         Evidence={ECO:0000250|UniProtKB:P03905};
CC   -!- SUBUNIT: Core subunit of respiratory chain NADH dehydrogenase (Complex
CC       I) which is composed of 45 different subunits.
CC       {ECO:0000250|UniProtKB:P03910}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:P03910}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR   EMBL; Y07726; CAA69015.1; -; Genomic_DNA.
DR   RefSeq; NP_007442.1; NC_001808.1.
DR   AlphaFoldDB; O03204; -.
DR   SMR; O03204; -.
DR   GeneID; 808102; -.
DR   CTD; 4538; -.
DR   OrthoDB; 996580at2759; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; ISS:UniProtKB.
DR   GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; ISS:UniProtKB.
DR   GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR   InterPro; IPR000260; NADH4_N.
DR   InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR   InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   PANTHER; PTHR43507; PTHR43507; 1.
DR   Pfam; PF01059; Oxidored_q5_N; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   PRINTS; PR01437; NUOXDRDTASE4.
DR   TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   NAD; Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW   Transport; Ubiquinone.
FT   CHAIN           1..459
FT                   /note="NADH-ubiquinone oxidoreductase chain 4"
FT                   /id="PRO_0000117919"
FT   TRANSMEM        22..42
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        60..80
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        95..112
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        113..133
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        145..165
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        194..214
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        224..244
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        257..277
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        284..303
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        308..330
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        351..371
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        391..411
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        436..456
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   459 AA;  51719 MW;  F25C961D11EC5DFE CRC64;
     MLKIIIPTLM LIPLTWLSKN SMIWINTTAY SLLISFISLP LLSQFNENSL NMSLTFFSDP
     LSAPLLVLTT WLLPLMIMAS QHHLSKEPLA RKKLYITMLI TLQTFLIMTF TATELIFFYI
     LFEATLIPTL IIITRWGNQT ERLNAGFYFL FYTLTGSLPL LIALTHIQNL TGSLNLLLIQ
     YSTQTLPSSW SNAFLWLACM MAFMVKMPLY GLHLWLPKAH VEAPIAGSMV LAAILLKLGG
     YGMLRITMIL NPLTSYMAYP FLMLSLWGMI MTSSICLRQT DLKSLIAYSS VSHMALVIVA
     VLIQTPWSYM GATALMIAHG LTSSVLFCLA NSNYERTHSR TMILARGLQT LLPLMAMWWL
     LASLTNLALP PTINLVGELF VVMSSFSWSN ITIILMGTNI IITALYSLYM LITTQRGKYT
     HHINNIKPSF TRENTLMALH LLPLLLLSLN PKIILGTLY