NU4M_CHLRE
ID NU4M_CHLRE Reviewed; 443 AA.
AC P20113;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 1.
DT 25-MAY-2022, entry version 106.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE EC=7.1.1.2;
DE AltName: Full=NADH dehydrogenase subunit 4;
GN Name=ND4; Synonyms=NAD4;
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OG Mitochondrion.
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cw15;
RX PubMed=1831072; DOI=10.1007/bf00355060;
RA Boer P.H., Gray M.W.;
RT "Short dispersed repeats localized in spacer regions of Chlamydomonas
RT reinhardtii mitochondrial DNA.";
RL Curr. Genet. 19:309-312(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cw15;
RX PubMed=2628173; DOI=10.1016/0378-1119(89)90429-0;
RA Ma D.-P., Yang Y.-W., King Y.T., Hasnain S.E.;
RT "Nucleotide sequence of cloned nad4 (urf4) gene from Chlamydomonas
RT reinhardtii mitochondrial DNA.";
RL Gene 85:363-370(1989).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cw15;
RX PubMed=2791036; DOI=10.1007/bf00411086;
RA Pratje E., Vahrenholz C., Buehler S., Michaelis G.;
RT "Mitochondrial DNA of Chlamydomonas reinhardtii: the ND4 gene encoding a
RT subunit of NADH dehydrogenase.";
RL Curr. Genet. 16:61-64(1989).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-75.
RX PubMed=3007117; DOI=10.1002/j.1460-2075.1986.tb04172.x;
RA Boer P.H., Gray M.W.;
RT "The URF 5 gene of Chlamydomonas reinhardtii mitochondria: DNA sequence and
RT mode of transcription.";
RL EMBO J. 5:21-28(1986).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 373-443.
RC STRAIN=cw15;
RA Vahrenholz C., Pratje E., Michaelis G., Dujon B.;
RT "Mitochondrial DNA of Chlamydomonas reinhardtii: sequence and arrangement
RT of URF5 and the gene for cytochrome oxidase subunit I.";
RL Mol. Gen. Genet. 201:213-224(1985).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR EMBL; X54860; CAA38640.1; -; Genomic_DNA.
DR EMBL; X66484; CAA47112.1; -; Genomic_DNA.
DR EMBL; X03464; CAA27182.1; -; Genomic_DNA.
DR EMBL; U03843; AAB93441.1; -; Genomic_DNA.
DR PIR; A48333; S37612.
DR RefSeq; NP_042565.1; NC_001638.1.
DR AlphaFoldDB; P20113; -.
DR SMR; P20113; -.
DR STRING; 3055.AAB93441; -.
DR TCDB; 3.D.1.6.4; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR GeneID; 801494; -.
DR KEGG; cre:ChrepMp02; -.
DR eggNOG; KOG4845; Eukaryota.
DR HOGENOM; CLU_007100_4_4_1; -.
DR OrthoDB; 996580at2759; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR InterPro; IPR001750; ND/Mrp_mem.
DR PANTHER; PTHR43507; PTHR43507; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR PRINTS; PR01437; NUOXDRDTASE4.
DR TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE 3: Inferred from homology;
KW Electron transport; Membrane; Mitochondrion; NAD; Respiratory chain;
KW Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT CHAIN 1..443
FT /note="NADH-ubiquinone oxidoreductase chain 4"
FT /id="PRO_0000117921"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 27..47
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..134
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 148..168
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 217..237
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 247..267
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 279..299
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..328
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 335..355
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 362..382
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 385..405
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 423..443
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 443 AA; 48737 MW; 2D1B9E3DCC244A5E CRC64;
MFISVLLILF ALCVTLIPEA HYHMVRVWSF VATIIPMWVV TWMWWNFDAS GHGLQMLVIL
GRSHLAFGID GVALSLMLLT TVLFPICMML LRTVAGFMTF ILLEVLVLSA LCVLDLLGFY
ILFEASLILL FLLIGRAPYG SLEAAYKIVL YTMAGSLVLL PTLFMIYSEC GTTNVLYMTC
AYNHQTVLGW GLLAVLAVKI PLMPVHLWLP EAHVAAPTAG SVLLAGVLLK LGGIGFLRFM
LPVVPEFCVS VFPLVSTLCL VSFLFSTLST LRQIDLKKIV AYSSIAHMSM VTLAIFSQSE
FSAYSSSFLM IAHGLISPAL FLIVGILYDR AHTKFILYFS GLGASMPIGS TLFFLFTLGN
LAFPLFPNFI AEVLCMVSIF AVHELLAYVF CVCQVLGAAY GFWAFNRVVH GLPRGPADVT
RTEFHTVLPL LIGAVWLGIK PMA
