NU4M_DANRE
ID NU4M_DANRE Reviewed; 460 AA.
AC Q9MIY1;
DT 06-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE EC=7.1.1.2 {ECO:0000250|UniProtKB:P03905};
DE AltName: Full=NADH dehydrogenase subunit 4;
GN Name=mt-nd4; Synonyms=mtnd4, nd4;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OG Mitochondrion.
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen {ECO:0000312|Proteomes:UP000000437};
RX PubMed=11691861; DOI=10.1101/gr.156801;
RA Broughton R.E., Milam J.E., Roe B.A.;
RT "The complete sequence of the zebrafish (Danio rerio) mitochondrial genome
RT and evolutionary patterns in vertebrate mitochondrial DNA.";
RL Genome Res. 11:1958-1967(2001).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) which catalyzes electron transfer from
CC NADH through the respiratory chain, using ubiquinone as an electron
CC acceptor. Essential for the catalytic activity and assembly of complex
CC I. {ECO:0000250|UniProtKB:P03905}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC Evidence={ECO:0000250|UniProtKB:P03905};
CC -!- SUBUNIT: Core subunit of respiratory chain NADH dehydrogenase (Complex
CC I) which is composed of 45 different subunits.
CC {ECO:0000250|UniProtKB:P03910}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:P03910}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR EMBL; AC024175; AAF74306.1; -; Genomic_DNA.
DR RefSeq; NP_059340.1; NC_002333.2.
DR AlphaFoldDB; Q9MIY1; -.
DR SMR; Q9MIY1; -.
DR STRING; 7955.ENSDARP00000087878; -.
DR PaxDb; Q9MIY1; -.
DR PRIDE; Q9MIY1; -.
DR Ensembl; ENSDART00000093618; ENSDARP00000087878; ENSDARG00000063917.
DR GeneID; 140534; -.
DR KEGG; dre:140534; -.
DR CTD; 4538; -.
DR ZFIN; ZDB-GENE-011205-10; mt-nd4.
DR eggNOG; KOG4845; Eukaryota.
DR GeneTree; ENSGT00730000111316; -.
DR HOGENOM; CLU_007100_4_0_1; -.
DR InParanoid; Q9MIY1; -.
DR OMA; ITRWGNQ; -.
DR OrthoDB; 996580at2759; -.
DR PhylomeDB; Q9MIY1; -.
DR TreeFam; TF343520; -.
DR Reactome; R-DRE-611105; Respiratory electron transport.
DR Reactome; R-DRE-6799198; Complex I biogenesis.
DR PRO; PR:Q9MIY1; -.
DR Proteomes; UP000000437; Mitochondrion MT.
DR Proteomes; UP000814640; Mitochondrion.
DR Bgee; ENSDARG00000063917; Expressed in brain and 20 other tissues.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; ISS:UniProtKB.
DR GO; GO:0048039; F:ubiquinone binding; IBA:GO_Central.
DR GO; GO:0009060; P:aerobic respiration; IBA:GO_Central.
DR GO; GO:0015990; P:electron transport coupled proton transport; IBA:GO_Central.
DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; ISS:UniProtKB.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR InterPro; IPR000260; NADH4_N.
DR InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR InterPro; IPR001750; ND/Mrp_mem.
DR PANTHER; PTHR43507; PTHR43507; 1.
DR Pfam; PF01059; Oxidored_q5_N; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR PRINTS; PR01437; NUOXDRDTASE4.
DR TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE 3: Inferred from homology;
KW Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW NAD; Reference proteome; Respiratory chain; Translocase; Transmembrane;
KW Transmembrane helix; Transport; Ubiquinone.
FT CHAIN 1..460
FT /note="NADH-ubiquinone oxidoreductase chain 4"
FT /id="PRO_0000117910"
FT TRANSMEM 20..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 61..81
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 94..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..134
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 148..168
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 195..215
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 225..245
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 258..278
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 285..304
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..330
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 351..371
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 380..400
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 436..456
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 460 AA; 51529 MW; 1615202B56513143 CRC64;
MLKVLIPTIM LFPTIWLSSS KWLWTTTTMN SFLIAFISLT WLKWTSDTGW NASNSYMAAD
PLSTPLLVLT CWLLPLMILA SQNHINSEPV NRQRMYITLL ASLQTFMIMA FGATKIIMFY
IMFEATLIPT LIIITRWGNQ AERLNAGTYF LFYTLAGSLP LLVALLLLQQ STGTLSMLVL
QYSDPLLLNS WGHKIWWAGC LIAFLVKMPL YGMHLWLPKA HVEAPVAGSM ILAAVLLKLG
GYGMMRMMVM LDPLSKQLAY PFIILALWGV IMTGLVCLRQ TDLKSLIAYS SVGHMGLVAG
GILIQTPWGF TGAIILMIAH GLTSSALFCL ANTSYERTHS RTMILARGLQ MVLPLATVWW
FIANLANLAL PPLPNLMGEL MIITALFNWS PWTIIITGMG TLITANYSLY MFLTSQRGSI
PEHITNLSPS HTREHLLMTL HLIPIILLML KPELMWGWCN
