NU4M_DICDI
ID NU4M_DICDI Reviewed; 540 AA.
AC O21047;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 104.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE EC=7.1.1.2;
DE AltName: Full=NADH dehydrogenase subunit 4;
GN Name=nad4; ORFNames=DDB_G0294048;
OS Dictyostelium discoideum (Slime mold).
OG Mitochondrion.
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=AX3;
RX PubMed=9000384; DOI=10.1007/s002940050179;
RA Ogawa S., Matsuo K., Angata K., Yanagisawa K., Tanaka Y.;
RT "Group-I introns in the cytochrome c oxidase genes of Dictyostelium
RT discoideum: two related ORFs in one loop of a group-I intron, a cox1/2
RT hybrid gene and an unusually large cox3 gene.";
RL Curr. Genet. 31:80-88(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX3;
RX PubMed=10821186; DOI=10.1007/pl00008685;
RA Ogawa S., Yoshino R., Angata K., Iwamoto M., Pi M., Kuroe K., Matsuo K.,
RA Morio T., Urushihara H., Yanagisawa K., Tanaka Y.;
RT "The mitochondrial DNA of Dictyostelium discoideum: complete sequence, gene
RT content and genome organization.";
RL Mol. Gen. Genet. 263:514-519(2000).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR EMBL; D16579; BAA21118.1; -; Genomic_DNA.
DR EMBL; AB000109; BAA78051.1; -; Genomic_DNA.
DR PIR; T43747; T43747.
DR RefSeq; NP_050069.1; NC_000895.1.
DR AlphaFoldDB; O21047; -.
DR SMR; O21047; -.
DR GeneID; 2193891; -.
DR KEGG; ddi:DidioMp02; -.
DR dictyBase; DDB_G0294048; nad4.
DR InParanoid; O21047; -.
DR OMA; ITRWGNQ; -.
DR PhylomeDB; O21047; -.
DR PRO; PR:O21047; -.
DR Proteomes; UP000002195; Mitochondrion.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR GO; GO:0048039; F:ubiquinone binding; IBA:GO_Central.
DR GO; GO:0009060; P:aerobic respiration; IBA:GO_Central.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR GO; GO:0015990; P:electron transport coupled proton transport; IBA:GO_Central.
DR InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR InterPro; IPR001750; ND/Mrp_mem.
DR PANTHER; PTHR43507; PTHR43507; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR PRINTS; PR01437; NUOXDRDTASE4.
DR TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE 3: Inferred from homology;
KW Electron transport; Membrane; Mitochondrion; NAD; Reference proteome;
KW Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW Transport; Ubiquinone.
FT CHAIN 1..540
FT /note="NADH-ubiquinone oxidoreductase chain 4"
FT /id="PRO_0000311825"
FT TRANSMEM 2..22
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 35..55
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 89..109
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 118..138
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 218..238
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 248..268
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 310..330
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 338..358
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 376..396
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 415..435
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 501..521
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 540 AA; 60234 MW; D6C54F38BA01742C CRC64;
MTIIAISIMN VVIGIAILGV ILRKKIMPNQ KFQRIFILGV QGILIVLSGI MLIGCEGTDI
INRISPYINM VTVYSNNVNI NIGYSIDGIS AIFIFLTIIL ILSCNLISIR VIKEKTEQKF
QIMLLLTEIL IINFFAATDL VQLYIVYEAT LIPMVIMIGV WGSRTEKKIA AFQILIYTLI
GSIFMLMSIG ILYSTLGTTD YIMIREYIDV LPENVRKLIF IGFFIGFAVK IPIAPLHLWL
LRAHVEAPTA GSVLLAGILL KLGGYGYIRY NIGLFPDLCE YYFPIIGGIC LISILYTGIA
TLTQLDVKRI VAYSSISHMN VIVLGLFSGV LQGIEGGIIL MIGHGVVSGG LFLCIGVIYD
RCKTRIVYAY NNLVHVMPIM AILFFLLVLG NIAFPITSNF VGELLIFIGL IKKNIIIAFF
SALSMIVTAI YSFWLYNRIF FVNEIIKREA NEVISSKGQI VADMNALFLI EDVMKKKEER
GIDDIGQPKE VKKEQLIYSD VNIFEFTSIS LMVIMMIIIG MKPSVVEGFI AINCLELISK
