NU4M_DROME
ID NU4M_DROME Reviewed; 446 AA.
AC P18931;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2017, sequence version 3.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE EC=7.1.1.2;
DE AltName: Full=NADH dehydrogenase subunit 4;
GN Name=mt:ND4; Synonyms=ND4; ORFNames=CG34085;
OS Drosophila melanogaster (Fruit fly).
OG Mitochondrion.
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Bretagne;
RX PubMed=3130291; DOI=10.1093/genetics/118.4.649;
RA Garesse R.;
RT "Drosophila melanogaster mitochondrial DNA: gene organization and
RT evolutionary considerations.";
RL Genetics 118:649-663(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=8825764; DOI=10.1111/j.1365-2583.1995.tb00032.x;
RA Lewis D.L., Farr C.L., Kaguni L.S.;
RT "Drosophila melanogaster mitochondrial DNA: completion of the nucleotide
RT sequence and evolutionary comparisons.";
RL Insect Mol. Biol. 4:263-278(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RA Wan K., Celniker S.;
RL Submitted (AUG-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) that is believed to belong to the
CC minimal assembly required for catalysis. Complex I functions in the
CC transfer of electrons from NADH to the respiratory chain. The immediate
CC electron acceptor for the enzyme is believed to be ubiquinone (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR EMBL; M37275; AAA69711.1; ALT_TERM; Genomic_DNA.
DR EMBL; U37541; AAC47819.1; -; Genomic_DNA.
DR EMBL; KJ947872; AIC64012.1; -; Genomic_DNA.
DR RefSeq; YP_009047274.1; NC_024511.2.
DR AlphaFoldDB; P18931; -.
DR SMR; P18931; -.
DR STRING; 7227.FBpp0100183; -.
DR PaxDb; P18931; -.
DR EnsemblMetazoa; FBtr0433500; FBpp0390632; FBgn0262952.
DR GeneID; 19893551; -.
DR KEGG; dme:Dmel_CG34085; -.
DR CTD; 4538; -.
DR FlyBase; FBgn0262952; mt:ND4.
DR VEuPathDB; VectorBase:FBgn0262952; -.
DR eggNOG; KOG4845; Eukaryota.
DR GeneTree; ENSGT00730000111316; -.
DR HOGENOM; CLU_007100_4_0_1; -.
DR InParanoid; P18931; -.
DR OrthoDB; 996580at2759; -.
DR PhylomeDB; P18931; -.
DR Reactome; R-DME-611105; Respiratory electron transport.
DR Reactome; R-DME-6799198; Complex I biogenesis.
DR GenomeRNAi; 19893551; -.
DR PRO; PR:P18931; -.
DR Proteomes; UP000000803; Mitochondrion.
DR Bgee; FBgn0262952; Expressed in Malpighian tubule and 15 other tissues.
DR ExpressionAtlas; P18931; baseline and differential.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; ISS:FlyBase.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR GO; GO:0048039; F:ubiquinone binding; IBA:GO_Central.
DR GO; GO:0009060; P:aerobic respiration; IBA:GO_Central.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR GO; GO:0015990; P:electron transport coupled proton transport; IBA:GO_Central.
DR InterPro; IPR000260; NADH4_N.
DR InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR InterPro; IPR001750; ND/Mrp_mem.
DR PANTHER; PTHR43507; PTHR43507; 1.
DR Pfam; PF01059; Oxidored_q5_N; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR PRINTS; PR01437; NUOXDRDTASE4.
PE 3: Inferred from homology;
KW Electron transport; Membrane; Mitochondrion; NAD; Reference proteome;
KW Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW Transport; Ubiquinone.
FT CHAIN 1..446
FT /note="NADH-ubiquinone oxidoreductase chain 4"
FT /id="PRO_0000117930"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..134
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 218..238
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 245..265
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 272..292
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 297..317
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 330..350
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 373..393
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 426..446
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 160
FT /note="Y -> L (in Ref. 1; AAA69711)"
FT /evidence="ECO:0000305"
FT CONFLICT 161
FT /note="L -> V (in Ref. 2; AAC47819)"
FT /evidence="ECO:0000305"
FT CONFLICT 199..200
FT /note="LV -> FS (in Ref. 1; AAA69711)"
FT /evidence="ECO:0000305"
FT CONFLICT 201
FT /note="H -> Y (in Ref. 1; AAA69711 and 2; AAC47819)"
FT /evidence="ECO:0000305"
FT CONFLICT 368
FT /note="Y -> S (in Ref. 1; AAA69711 and 2; AAC47819)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 446 AA; 51448 MW; F608E91100235C8E CRC64;
MLKIIFFLLF LIPFCFINNM YWMVQIMMFF ISFIFLLMNN FMNYWSEISY FLGCDMLSYG
LILLSLWICS LMLLASEMIN KHNNYKNLFL LNIIILLLLL ILTFSSMSLF MFYLFFESSL
IPTLFLILGW GYQPERLQAG LYLLFYTLLV SLPMLIGIFY LMNKIGSMNF YLMNNFMFNY
DLLYFCLLCA FLVKMPMFLV HLWLPKAHVE APVSGSMILA GIMLKLGGYG MLRVISFLQL
MNLKYSFVWI SISLVGGVLV SLVCLRQTDL KALIAYSSVA HMGIVLSGLL TMTYWGLCGS
YTLMIAHGLC SSGLFCLANV SYERLGSRSM LINKGLLNFM PSMTLWWFLL SSANMAAPPT
LNLLGEIYLL NSIVSWSWIS MILLSFLSFF SAAYTLYLYS FSQHGKLFSG VYSFSSGKIR
EYLLMLLHWL PLNLLILKSE SFMLWL
