ID   NU4M_EMEND              Reviewed;         221 AA.
AC   P03914;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE            EC=7.1.1.2;
DE   AltName: Full=NADH dehydrogenase subunit 4;
DE   Flags: Fragment;
GN   Name=nd4; Synonyms=ndhD;
OS   Emericella nidulans (Aspergillus nidulans).
OG   Mitochondrion.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Nidulantes.
OX   NCBI_TaxID=162425;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=pabaA1 biA1;
RX   PubMed=6290989; DOI=10.1093/nar/10.15.4783;
RA   Netzker R., Koechel H.G., Basak N., Kuentzel H.;
RT   "Nucleotide sequence of Aspergillus nidulans mitochondrial genes coding for
RT   ATPase subunit 6, cytochrome oxidase subunit 3, seven unidentified
RT   proteins, four tRNAs and L-rRNA.";
RL   Nucleic Acids Res. 10:4783-4794(1982).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR   EMBL; J01390; AAA99203.1; -; Genomic_DNA.
DR   EMBL; X04161; CAA27771.1; -; Genomic_DNA.
DR   PIR; A00443; QXAS4M.
DR   AlphaFoldDB; P03914; -.
DR   SMR; P03914; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR   InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR   InterPro; IPR001750; ND/Mrp_mem.
DR   PANTHER; PTHR43507; PTHR43507; 1.
DR   Pfam; PF00361; Proton_antipo_M; 1.
DR   PRINTS; PR01437; NUOXDRDTASE4.
PE   3: Inferred from homology;
KW   Electron transport; Membrane; Mitochondrion; NAD; Respiratory chain;
KW   Translocase; Transmembrane; Transmembrane helix; Transport; Ubiquinone.
FT   CHAIN           <1..221
FT                   /note="NADH-ubiquinone oxidoreductase chain 4"
FT                   /id="PRO_0000117933"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        34..54
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        61..81
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        100..120
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        140..160
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        185..205
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   NON_TER         1
SQ   SEQUENCE   221 AA;  24444 MW;  86AB34082584D3E4 CRC64;
     ASINYTYIIY VIGVITILYA SFSTLRTIDI KELIAYSSVS HAAVYLIGAF SNTIQGIEGS
     IALGLAHGFV SSGLFICAGG ILYDRSSTRL ITYYRGMAQI MPIFSVLFFI LALGNSGTPL
     TLNFIGEFMS LYGVFERMPI LGVLASTSIV FSAAYTIFMY NRIVFGGSYS IYFRENIGDV
     TRREFIMLLV FVILTVLFGI YPAPILDGLH YSVSYLIYNI N