NU4M_LEMCA
ID NU4M_LEMCA Reviewed; 459 AA.
AC Q34878; Q8HCB2; Q8LX25;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 29-MAR-2005, sequence version 2.
DT 25-MAY-2022, entry version 82.
DE RecName: Full=NADH-ubiquinone oxidoreductase chain 4;
DE EC=7.1.1.2 {ECO:0000250|UniProtKB:P03905};
DE AltName: Full=NADH dehydrogenase subunit 4;
GN Name=MT-ND4; Synonyms=MTND4, NADH4, ND4;
OS Lemur catta (Ring-tailed lemur).
OG Mitochondrion.
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Strepsirrhini; Lemuriformes;
OC Lemuridae; Lemur.
OX NCBI_TaxID=9447;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Isolate JP27, and Isolate JP52;
RX PubMed=12719521; DOI=10.1073/pnas.1031673100;
RA Pastorini J., Thalmann U., Martin R.D.;
RT "A molecular approach to comparative phylogeography of extant Malagasy
RT lemurs.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:5879-5884(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=12034869; DOI=10.1073/pnas.102164299;
RA Arnason U., Adegoke J.A., Bodin K., Born E.W., Esa Y.B., Gullberg A.,
RA Nilsson M., Short R.V., Xu X., Janke A.;
RT "Mammalian mitogenomic relationships and the root of the eutherian tree.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:8151-8156(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 308-459.
RX PubMed=3146681; DOI=10.1093/oxfordjournals.molbev.a040524;
RA Hayasaka K., Gojobori T., Horai S.;
RT "Molecular phylogeny and evolution of primate mitochondrial DNA.";
RL Mol. Biol. Evol. 5:626-644(1988).
CC -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC NADH dehydrogenase (Complex I) which catalyzes electron transfer from
CC NADH through the respiratory chain, using ubiquinone as an electron
CC acceptor. Essential for the catalytic activity and assembly of complex
CC I. {ECO:0000250|UniProtKB:P03905}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC Evidence={ECO:0000250|UniProtKB:P03905};
CC -!- SUBUNIT: Core subunit of respiratory chain NADH dehydrogenase (Complex
CC I) which is composed of 45 different subunits.
CC {ECO:0000250|UniProtKB:P03910}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:P03910}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR EMBL; AF224569; AAN64756.1; -; Genomic_DNA.
DR EMBL; AF224570; AAN64760.1; -; Genomic_DNA.
DR EMBL; AJ421451; CAD13430.2; ALT_SEQ; Genomic_DNA.
DR EMBL; M22657; AAA69751.1; -; Genomic_DNA.
DR PIR; I57440; I57440.
DR RefSeq; NP_659297.3; NC_004025.1.
DR AlphaFoldDB; Q34878; -.
DR SMR; Q34878; -.
DR GeneID; 804965; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; ISS:UniProtKB.
DR GO; GO:0006120; P:mitochondrial electron transport, NADH to ubiquinone; ISS:UniProtKB.
DR GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR InterPro; IPR000260; NADH4_N.
DR InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR InterPro; IPR001750; ND/Mrp_mem.
DR PANTHER; PTHR43507; PTHR43507; 1.
DR Pfam; PF01059; Oxidored_q5_N; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR PRINTS; PR01437; NUOXDRDTASE4.
DR TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE 3: Inferred from homology;
KW Electron transport; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW NAD; Respiratory chain; Translocase; Transmembrane; Transmembrane helix;
KW Transport; Ubiquinone.
FT CHAIN 1..459
FT /note="NADH-ubiquinone oxidoreductase chain 4"
FT /id="PRO_0000117947"
FT TRANSMEM 22..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 90..110
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 145..165
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 194..214
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 224..244
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 257..277
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 284..303
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..330
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 351..371
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 391..411
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 232..233
FT /note="AA -> PP (in Ref. 2; CAD13430)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 459 AA; 52087 MW; 23DCF062D4150B02 CRC64;
MLKIIIPTIM LFPVTWCSSS PMIWINTTLH SLLISLAGLL FLNQFNDSNN NFSLTFFSDS
LSSPLVVLTM WLLPLMIMAS QHHLKKEPWT LKKLYISMLI FLQMFLIMTF TATELILFYI
LFEATLIPTL IIITRWGNQT ERLNAGLYFL FYTLIGSLPL LVALIHIQNY LGSLNMLTMS
FCFQELSNSW SSNLLWMACI MAFMVKMPLY GLHLWLPKAH VEAPIAGSMV LAAVLLKLGG
YGMMRITMIL NPMTKYMAYP FLMLCLWGMI MTSSICLRQT DLKSLIAYSS VSHMALVIVA
ILIQTPWSFM GATILMIAHG LTSSMLFCLA NSNYERIHSR TMLLARGIQT ILPLMATWWL
LASLTNLALP PSINLIGELF VTMASFSWSN ITIILMGLNM LITALYSLYM LTTTQRGKLT
YHSHNLNPSF TRENTLMSMH MLPLLLFTLN PKIILGPTY
